Anti-Hu CD326 Purified EPCAM Monoclonal Antibody

Product Name

Anti-Hu CD326 Purified EPCAM Monoclonal Antibody

View all EpCAM/TROP1 Antibodies

SKU/Catalog Number

M00276

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD326 Purified EPCAM Monoclonal Antibody (Catalog# M00276). Tested in Flow Cytometry, IHC-P, IP, WB, IHC-F, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD326 Purified EPCAM Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00276)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

VU-1D9

Isotype

Mouse IgG1

Immunogen

Small cell lung carcinoma cell line H69. The mouse monoclonal antibody VU-1D9 recognizes an extracellular epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00276 is reactive to EPCAM in Human

Applications

M00276 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

34.932kDa

Background of EpCAM/TROP1

CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections): 5-10 μg/ml; positive control: colon epithelium, antigen retrieval: heat (sodium citrate) + trypsin.
Immunocytochemistry: 4 μg/ml.
Western blotting: 1-4 μg/ml, non-reducing conditions.
Flow cytometry: 1-3 μg/ml.
Immunoprecipitation: 1-2 μg / 100-500 μg of protein in 1 ml lysate.

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of EPCAM using Anti-Hu CD326 Purified EPCAM Monoclonal Antibody (M00276).

Immunohistochemistry staining of human prostate (paraffin sections) using anti-CD326 (VU-1D9).
Commercially tested by LifeSpan BioSciences.
For more protocol information of IHC

Gene/Protein Information For EPCAM (Source: Uniprot.org, NCBI)

Alternative Names

17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A EPCAM DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1 epithelial cell adhesion molecule epithelial cell adhesion molecule|adenocarcinoma-associated antigen|cell surface glycoprotein Trop-1|epithelial glycoprotein 314|human epithelial glycoprotein-2|major gastrointestinal tumor-associated protein GA733-2|membrane component, chromosome 4, surface marker (35kD glycoprotein)|trophoblast cell surface antigen 1|tumor-associated calcium signal transducer 1

For more info on EPCAM, check out the EPCAM Infographic

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In this infographic, you will see the following information for EPCAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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