Anti-Hu CD82 Purified Monoclonal Antibody

Product Name

Anti-Hu CD82 Purified Monoclonal Antibody

View all CD82/Kai-1 Antibodies

SKU/Catalog Number

M02300-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD82 Purified Monoclonal Antibody (Catalog# M02300-1). Tested in Flow Cytometry, IP, WB, IHC-P, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD82 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02300-1)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

C33

Isotype

Mouse IgG2a

Immunogen

C91/PL (human HTLV-1+ T cell line). The mouse monoclonal antibody C33 recognizes an extracellular/luminal epitope of CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M02300-1 is reactive to CD82 in Human

Applications

M02300-1 is guaranteed for Flow Cytometry, IP, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

29.626kDa

Background of CD82/Kai-1

CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1-4 µg/ml

Validation Images & Assay Conditions

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Figure 1. Flow Cytometry validation of CD82 using Anti-Hu CD82 Purified Monoclonal Antibody (M02300-1).

Separation of human CD82 positive lymphocytes (red-filled) from CD82 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD82 (C33) purified antibody (concentration in sample 1 μg/ml) GAM APC.

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Figure 2. Flow Cytometry validation of CD82 using Anti-Hu CD82 Purified Monoclonal Antibody (M02300-1).

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD82 (C33) purified antibody (concentration in sample 1 μg/ml) GAM APC.

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Figure 3. Immunohistochemistry validation of CD82 using Anti-Hu CD82 Purified Monoclonal Antibody (M02300-1).

Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33).
Commercially tested by LifeSpan BioSciences.
For more protocol information of IHC

Gene/Protein Information For CD82 (Source: Uniprot.org, NCBI)

Alternative Names

CD82 antigenC33; CD82 molecule; CD82; IA4; IA4C33 antigen; Inducible membrane protein R2; KAI1; KAI1GR15; kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyteantigen, antigen detected by monoclonal and IA4)); Metastasis suppressor Kangai-1; R2; SAR2; SAR2tetraspanin-27; ST6; ST6tspan-27; Suppressor of tumorigenicity 6 protein; Tetraspanin-27; TSPAN27; Tspan-27; TSPAN274F9 CD82 4F9, C33, GR15, IA4, KAI1, R2, SAR2, ST6, TSPAN27 CD82 molecule CD82 antigen|C33 antigen|inducible membrane protein R2|kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4))|metastasis suppressor Kangai-1|tetraspanin-27|tspan-27

For more info on CD82, check out the CD82 Infographic

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In this infographic, you will see the following information for CD82: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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