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Product Info Summary
| SKU: | A03485 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-RHOG Antibody
SKU/Catalog Number
A03485
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RHOG Antibody (Catalog # A03485). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
RHOG antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-RHOG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03485)
Host
Rabbit
Contents
RHOG antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RHOG antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOG. The immunogen is located within amino acids 40 - 90 of RHOG.
Cross-reactivity
RHOG antibody is human, mouse and rat reactive.
Reactive Species
A03485 is reactive to RHOG in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
21.3 kDa
Background of RHOG
The ras homolog family member G (RHOG), also known as rho-related GTP-binding protein, is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades (1). RHOG controls a pathway that requires the microtubule network and activates Rac1 and Cdc42Hs independently of their growth factor signaling pathway (2). RHOG is also involved in the transcriptional regulation of interferon-gamma and nuclear factor of activated T cells (NFAT) and the regulation of the actin skeleton in lymphocytes (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03485 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RHOG antibody can be used for detection of RHOG by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RHOG in human kidney tissue lysate with RHOG antibody at 1 μg/ml.
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Immunohistochemistry of RHOG in mouse kidney tissue with RHOG antibody at 5 μg/ml.
Specific Publications For Anti-RHOG Antibody (A03485)
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