Anti-Bcl2 alpha Antibody

Bcl-2 antibody

Boster Bio Anti-Bcl2 alpha Antibody catalog # RP1003. Tested in ELISA, WB applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: RP1003
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-Bcl2 alpha Antibody

View all Bcl-2 Antibodies

SKU/Catalog Number

RP1003

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Bcl2 alpha Antibody catalog # RP1003. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Bcl2 alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1003)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human BCL-2 recombinant protein (Position: M1-D211).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1003 is reactive to BCL2 in Human

Applications

RP1003 is guaranteed for ELISA, WB Boster Guarantee

Background of Bcl-2

Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t (14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For BCL2 (Source: Uniprot.org, NCBI)

Uniprot ID

P10415

Gene ID

596

Gene Name

BCL2

Full Name

Apoptosis regulator Bcl-2

Weight

26.266kDa

Superfamily

Bcl-2 family

Alternative Names

apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl2; Bcl-2 BCL2 Bcl-2, PPP1R50 BCL2 apoptosis regulator apoptosis regulator Bcl-2|B-cell CLL/lymphoma 2|protein phosphatase 1, regulatory subunit 50

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BCL2, check out the BCL2 Infographic

BCL2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BCL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

RP1003 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Scutellarein selectively targets multiple myeloma cells by increasing mitochondrial superoxide production and activating intrinsic apoptosis pathway

Long J,Liu Z,Hui L.Anti-tumor effect and mechanistic study of elemene on pancreatic carcinoma.BMC Complement Altern Med.2019 Jun 18;19(1):133.doi: 10.1186/s12906-019-2544-2.PMID:31215421;PMCID:PMC6582541.
Species: Human,Mouse
RP1003 usage in article: APP:WB, SAMPLE:PANCREATIC TISSUE, DILUTION:1:500

Chen M,Wang Z,Zhou W,Lu C,Ji T,Yang W,Jin Z,Tian Y,Lei W,Wu S,Fu Q,Wu Z,Wu X,Han M,Fang M,Yang Y.SIRT1/PGC-1α signaling activation by mangiferin attenuates cerebral hypoxia/reoxygenation injury in neuroblastoma cells.Eur J Pharmacol.2021 Jun 8:174236.doi:10.1016/j.ejphar.2021.174236.Epub ahead of print.PMID:34116043.
Species: Mouse
RP1003 usage in article: APP:WB, SAMPLE:N2A CELL, DILUTION:1:500

Junyi Tang,Kehua Fang,Chang Li,Xiaotian Chang,"ARHGEF10L Promotes Cervical Tumorigenesis via RhoA-Mediated Signaling",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 6683264,11 pages,2021.https://doi.org/10.1155/2021/6683264
Species: Human
RP1003 usage in article: APP:WB, SAMPLE:HELA CELL, DILUTION:NA

Zhang Q,Duan HX,Li RL,Sun JY,Liu J,Peng W,Wu CJ,Gao YX. Inducing Apoptosis and Suppressing Inflammatory Reactions in Synovial Fibroblasts are Two Important Ways for Guizhi-Shaoyao-Zhimu Decoction Against Rheumatoid Arthritis. J Inflamm Res.2021 Jan 26;14:
Species: Human
RP1003 usage in article: APP:WB, SAMPLE:MH7A CELL, DILUTION:1:300

Cao X,Fang X,Malik WS,He Y,Li X,Xie M,Sun W,Xu Y,Liu X. TRB3 interacts with ERK and JNK and contributes to the proliferation, apoptosis, and migration of lung adenocarcinoma cells. J Cell Physiol.2020 Jan;235(1): 538-547.doi:10.1002/jcp.28993.Epub 2019 Ju
Species: Human
RP1003 usage in article: APP:WB, SAMPLE:LUNG TISSUE,A549 CELL AND H1299 CELL, DILUTION:NA

Liu L,Liu F,Sun Z,Peng Z,You T,Yu Z. LncRNA NEAT1 promotes apoptosis and inflammation in LPS-induced sepsis models by targeting miR-590-3p. Exp Ther Med.2020 Oct; 20(4):3290-3300. doi:10.3892/etm.2020.9079. Epub 2020 Jul 29.PMID:32855700;PMCID:PMC7444425.
Species: Human
RP1003 usage in article: APP:WB, SAMPLE: H9C2 CELL, DILUTION:1:500

Chen H,Sheng H,Zhao Y,Zhu G.Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway.Med Sci Monit.2020 Dec 31;26:e928403.doi:10.12659/MSM.928403.PMID:33382
Species: Human,Mouse

Oleanolic acid from Prunella Vulgaris L. Induces SPC-A-1 cell line apoptosis via regulation of Bax, Bad and Bcl-2 Expression

Baicalin attenuates alcoholic liver injury through modulation of hepatic oxidative stress, inflammation and sonic hedgehog pathway in rats

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16 Customer Q&As for Anti-Bcl2 alpha Antibody

Question

Is a blocking peptide available for product anti-Bcl2 alpha antibody (RP1003)?

Verified Customer

Verified customer

Asked: 2020-03-10

Answer

We do provide the blocking peptide for product anti-Bcl2 alpha antibody (RP1003). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-10

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thyroid gland using anti-Bcl2 alpha antibody RP1003. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-16

Question

Do you have a BSA free version of anti-Bcl2 alpha antibody RP1003 available?

Verified Customer

Verified customer

Asked: 2019-09-10

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bcl2 alpha antibody RP1003 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-10

Question

Is this RP1003 anti-Bcl2 alpha antibody reactive to the isotypes of BCL2?

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

The immunogen of RP1003 anti-Bcl2 alpha antibody is E. coli-derived human BCL-2 recombinant protein(Position: M1-D211). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-23

Question

See below the WB image, lot number and protocol we used for thyroid gland using anti-Bcl2 alpha antibody RP1003. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-22

Question

I have a question about product RP1003, anti-Bcl2 alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1003 anti-Bcl2 alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-09

Question

We need to test anti-Bcl2 alpha antibody RP1003 on human thyroid gland for research purposes, then I may be interested in using anti-Bcl2 alpha antibody RP1003 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-05-09

Answer

The products we sell, including anti-Bcl2 alpha antibody RP1003, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-09

Question

I see that the anti-Bcl2 alpha antibody RP1003 works with ELISA, what is the protocol used to produce the result images on the product page?

S. Collins

Verified customer

Asked: 2018-11-26

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-11-26

Question

Will RP1003 anti-Bcl2 alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-11-23

Answer

As indicated on the product datasheet, RP1003 anti-Bcl2 alpha antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-11-23

Question

We have seen staining in human thyroid gland. Any tips? Is anti-Bcl2 alpha antibody supposed to stain thyroid gland positively?

Verified Customer

Verified customer

Asked: 2018-09-28

Answer

From literature thyroid gland does express BCL2. From Uniprot.org, BCL2 is expressed in thyroid gland, testis, among other tissues. Regarding which tissues have BCL2 expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-09-28

Question

I was wanting to use your anti-Bcl2 alpha antibody for ELISA for human thyroid gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human thyroid gland identification?

Verified Customer

Verified customer

Asked: 2017-10-27

Answer

It shows on the product datasheet, RP1003 anti-Bcl2 alpha antibody has been tested for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human thyroid gland in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-27

Question

We are currently using anti-Bcl2 alpha antibody RP1003 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2017-06-08

Answer

The anti-Bcl2 alpha antibody (RP1003) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-08

Question

Will anti-Bcl2 alpha antibody RP1003 work on primate WB with thyroid gland?

Z. Carter

Verified customer

Asked: 2015-07-27

Answer

Our lab technicians have not tested anti-Bcl2 alpha antibody RP1003 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Bcl2 alpha antibody RP1003 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate thyroid gland in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-07-27

Question

Does anti-Bcl2 alpha antibody RP1003 work for ELISA with thyroid gland?

S. Mitchell

Verified customer

Asked: 2015-03-24

Answer

According to the expression profile of thyroid gland, BCL2 is highly expressed in thyroid gland. So, it is likely that anti-Bcl2 alpha antibody RP1003 will work for ELISA with thyroid gland.

Boster Scientific Support

Answered: 2015-03-24

Question

My boss were content with the WB result of your anti-Bcl2 alpha antibody. However we have seen positive staining in testis mitochondrion outer membrane using this antibody. Is that expected? Could you tell me where is BCL2 supposed to be expressed?

J. Zhao

Verified customer

Asked: 2014-12-09

Answer

According to literature, testis does express BCL2. Generally BCL2 expresses in mitochondrion outer membrane. Regarding which tissues have BCL2 expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2014-12-09

Question

Our lab used your anti-Bcl2 alpha antibody for ELISA on thyroid gland last year. I am using human, and We are going to use the antibody for WB next. We want examining thyroid gland as well as testis in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

A. Zhao

Verified customer

Asked: 2013-11-19

Answer

I have checked the website and datasheets of our anti-Bcl2 alpha antibody and I see that RP1003 has been tested on human in both ELISA and WB. Thus RP1003 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-11-19

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Total: $315

SKU:RP1003

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RP1003
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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