Anti-Bcl2 alpha Antibody

Boster Bio Anti-Bcl2 alpha Antibody catalog # RP1003. Tested in ELISA, WB applications. This antibody reacts with Human. Cited in 161 publication(s).

Product Info Summary

SKU: RP1003
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-Bcl2 alpha Antibody

See all Bcl-2 products

SKU/Catalog Number

RP1003

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Bcl2 alpha Antibody catalog # RP1003. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Bcl2 alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1003)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Immunogen

E. coli-derived human BCL-2 recombinant protein (Position: M1-D211).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1003 is reactive to BCL2 in Human

Applications

RP1003 is guaranteed for ELISA, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For BCL2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P10415

Gene ID

596

Gene Name

BCL2

Full Name

Apoptosis regulator Bcl-2

Weight

26.266kDa

Superfamily

Bcl-2 family

Alternative Names

apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl2; Bcl-2 BCL2 Bcl-2, PPP1R50 BCL2 apoptosis regulator apoptosis regulator Bcl-2|B-cell CLL/lymphoma 2|protein phosphatase 1, regulatory subunit 50

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BCL2, check out the BCL2 Infographic

BCL2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BCL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

RP1003 has been cited in 161 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Long J,Liu Z,Hui L.Anti-tumor effect and mechanistic study of elemene on pancreatic carcinoma.BMC Complement Altern Med.2019 Jun 18;19(1):133.doi: 10.1186/s12906-019-2544-2.PMID:31215421;PMCID:PMC6582541.
Species: Human,Mouse
RP1003 usage in article: APP:WB, SAMPLE:PANCREATIC TISSUE, DILUTION:1:500

Chen M,Wang Z,Zhou W,Lu C,Ji T,Yang W,Jin Z,Tian Y,Lei W,Wu S,Fu Q,Wu Z,Wu X,Han M,Fang M,Yang Y.SIRT1/PGC-1α signaling activation by mangiferin attenuates cerebral hypoxia/reoxygenation injury in neuroblastoma cells.Eur J Pharmacol.2021 Jun 8:174236.doi:10.1016/j.ejphar.2021.174236.Epub ahead of print.PMID:34116043.
Species: Mouse
RP1003 usage in article: APP:WB, SAMPLE:N2A CELL, DILUTION:1:500

Junyi Tang,Kehua Fang,Chang Li,Xiaotian Chang,"ARHGEF10L Promotes Cervical Tumorigenesis via RhoA-Mediated Signaling",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 6683264,11 pages,2021.https://doi.org/10.1155/2021/6683264
Species: Human
RP1003 usage in article: APP:WB, SAMPLE:HELA CELL, DILUTION:NA

Zhang Q,Duan HX,Li RL,Sun JY,Liu J,Peng W,Wu CJ,Gao YX. Inducing Apoptosis and Suppressing Inflammatory Reactions in Synovial Fibroblasts are Two Important Ways for Guizhi-Shaoyao-Zhimu Decoction Against Rheumatoid Arthritis. J Inflamm Res.2021 Jan 26;14:
Species: Human
RP1003 usage in article: APP:WB, SAMPLE:MH7A CELL, DILUTION:1:300

Cao X,Fang X,Malik WS,He Y,Li X,Xie M,Sun W,Xu Y,Liu X. TRB3 interacts with ERK and JNK and contributes to the proliferation, apoptosis, and migration of lung adenocarcinoma cells. J Cell Physiol.2020 Jan;235(1): 538-547.doi:10.1002/jcp.28993.Epub 2019 Ju
Species: Human
RP1003 usage in article: APP:WB, SAMPLE:LUNG TISSUE,A549 CELL AND H1299 CELL, DILUTION:NA

Liu L,Liu F,Sun Z,Peng Z,You T,Yu Z. LncRNA NEAT1 promotes apoptosis and inflammation in LPS-induced sepsis models by targeting miR-590-3p. Exp Ther Med.2020 Oct; 20(4):3290-3300. doi:10.3892/etm.2020.9079. Epub 2020 Jul 29.PMID:32855700;PMCID:PMC7444425.
Species: Human
RP1003 usage in article: APP:WB, SAMPLE: H9C2 CELL, DILUTION:1:500

Chen H,Sheng H,Zhao Y,Zhu G.Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway.Med Sci Monit.2020 Dec 31;26:e928403.doi:10.12659/MSM.928403.PMID:33382
Species: Human,Mouse

Oleanolic acid from Prunella Vulgaris L. Induces SPC-A-1 cell line apoptosis via regulation of Bax, Bad and Bcl-2 Expression

Baicalin attenuates alcoholic liver injury through modulation of hepatic oxidative stress, inflammation and sonic hedgehog pathway in rats

Antitumor effects of Laminaria extract fucoxanthin on lung cancer

Recombinant Newcastle disease virus expressing human IFN%u2010%u03BB1 (rL%u2010hIFN%u2010%u03BB1)%u2010induced apoptosis of A549 cells is connected to endoplasmic reticulum stress %u2026

The inhibition effect of triptolide on human endometrial carcinoma cell line HEC-1B: A in vitro and in vivo studies

Ibutilide treatment protects against ER stress induced apoptosis by regulating calumenin expression in tunicamycin treated cardiomyocytes

NOB1 silencing inhibits the growth and metastasis of laryngeal cancer cells through the regulation of JNK signaling pathway

Transforming growth factor-beta1 suppresses hepatocellular carcinoma proliferation via activation of Hippo signaling

Reactive oxygen species-induced apoptosis in PC12 cells and protective effect of bilobalide

Myricetin exhibits anti-glioma potential by inducing mitochondrial-mediated apoptosis, cell cycle arrest, inhibition of cell migration and ROS generation

Copper ion attenuated the antiproliferative activity of di-2-pyridylhydrazone dithiocarbamate derivative; however, there was a lack of correlation between ROS %u2026

Laminarin%u2011induced apoptosis in human colon cancer LoVo cells

Reactive oxygen species mediate isoalantolactone-induced apoptosis in human prostate cancer cells

Ginsenoside-Rh2 inhibits proliferation and induces apoptosis of human gastric cancer SGC-7901 side population cells

Inhibition of cpu0213, a dual endothelin receptor antagonist, on apoptosis via nox4-dependent ros in hk-2 cells

Transcriptome analysis of Spodoptera frugiperda Sf9 cells reveals putative apoptosis-related genes and a preliminary apoptosis mechanism induced by %u2026

Correlation of acetabular chondrocyte apoptosis with caspase-3 and BCL-2 expression in developmental dislocations of the hip

Inhibitory effects of vitamin E on osteocyte apoptosis and DNA oxidative damage in bone marrow hemopoietic cells at early stage of steroid-induced femoral %u2026

Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3-E1 cells

N-acetylcysteine reduces oxidative stress, nuclear factor-?B activity and cardiomyocyte apoptosis in heart failure

Fibroblast growth factor 21 protects rat cardiomyocytes from endoplasmic reticulum stress by promoting the fibroblast growth factor receptor 1-extracellular signal-regulated kinase 1/2 signaling pathway

Ailanthone induces G2/M cell cycle arrest and apoptosis of SGC-7901 human gastric cancer cells

1-calcium phosphate-uracil, a synthesized pyrimidine derivative agent, has anti-proliferative, pro-apoptotic and anti-invasion effects on multiple tumor cell lines

CCAAT/enhancer binding protein homologous protein knockdown alleviates hypoxia-induced myocardial injury in rat cardiomyocytes exposed to high glucose

Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian Granular Cells of Mice

Hepatitis E Virus Induces Hepatocyte Apoptosis via Mitochondrial Pathway in Mongolian Gerbils

Downregulation of BRAF-activated non-coding RNA suppresses the proliferation, migration and invasion, and induces apoptosis of hepatocellular carcinoma cells

Blockade of sonic hedgehog signaling decreases viability and induces apoptosis in retinoblastoma cells: The key role of the PI3K/Akt pathway

Siva 1 inhibits proliferation, migration and invasion by phosphorylating Stathmin in ovarian cancer cells

RKTG overexpression inhibits proliferation and induces apoptosis of human leukemia cells via suppression of the ERK and PI3K/AKT signaling pathways

Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats

BMP-7 suppresses excessive scar formation by activating the BMP-7/Smad1/5/8 signaling pathway

Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure

Cardioprotective effects of traditional Chinese medicine Guanmaitong on acute myocardial infarction

Vanadium toxicity in the thymic development

Comparative Study on the Protective Effects of Salidroside and Hypoxic Preconditioning for Attenuating Anoxia-Induced Apoptosis in Pheochromocytoma (PC12) Cells

Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis

The Effects of Fasudil at Different Doses on Acute Myocardial Infarction in Rats

Effects of As2O3nanoparticles on cell growth and apoptosis of NB4 cells

Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats

Effect of curcumin on Bcl-2 and Bax expression in nude mice prostate cancer.

Adipose-Derived Mesenchymal Stem Cells Transplantation Alleviates Renal Injury in Streptozotocin-Induced Diabetic Nephropathy

A preliminary study of the effect of ECRG4 overexpression on the proliferation and apoptosis of human laryngeal cancer cells and the underlying mechanisms

Delivery of the co-expression plasmid pEndo-Si-Stat3 by attenuatedSalmonellaserovartyphimuriumfor prostate cancer treatment

Expression of Survivin in pancreatic cancer and its correlation to expression of Bcl-2

In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures

Effects of garlicin on apoptosis in rat model of colitis

Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells

Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells

Knockdown of High Mobility Group-Box 3 (HMGB3) Expression Inhibits Proliferation, Reduces Migration, and Affects Chemosensitivity in Gastric Cancer Cells

Valproate Attenuates Nitroglycerin-Induced Trigeminovascular Activation by Preserving Mitochondrial Function in a Rat Model of Migraine

A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo

Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway

Radix Ilicis Pubescentis total flavonoids combined with mobilization of bone marrow stem cells to protect against cerebral ischemia/reperfusion injury

Astaxanthin Inhibits Acetaldehyde-Induced Cytotoxicity in SH-SY5Y Cells by Modulating Akt/CREB and p38MAPK/ERK Signaling Pathways

Nek7 is overexpressed in hepatocellular carcinoma and promotes hepatocellular carcinoma cell proliferation in vitro and in vivo

Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-?B Signaling Pathway

Mesenchymal stem cells promote colorectal cancer progression through AMPK/mTOR-mediated NF-?B activation

Yangjing Capsule Ameliorates Spermatogenesis in Male Mice Exposed to Cyclophosphamide

Fenofibrate, a PPAR? agonist, protect proximal tubular cells from albumin-bound fatty acids induced apoptosis via the activation of NF-kB

Hippocampal mitogen-activated protein kinase activation is associated with intermittent hypoxia in a rat model of obstructive sleep apnea syndrome

Expression of TP53, BCL-2, and VEGFA Genes in Esophagus Carcinoma and its Biological Significance

Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats

Effects of intracerebral hemorrhage and subsequent minimally invasive hematoma aspiration on expression of apoptosisrelated genes in rats.

Effect of ligustilide on Ang II-induced hypertrophy in cardiomyocytes and the potential mechanisms

Wang Y, Li Y, Yang W, Gao S, Lin J, Wang T, Zhou K, Hu H. Am J Transl Res. 2018 Mar 15;10(3):796-805. eCollection 2018. Ginsenoside Rb1 inhibit apoptosis in rat model of Alzheimer’s disease induced by Aβ1-40

Qi QM, Xue YC, Lv J, Sun D, Du JX, Cai SQ, Li YH, Gu TC, Wang MB. Oncol Lett. 2018 May;15(5):6400-6408. doi: 10.3892/ol.2018.8177. Epub 2018 Mar 5. Ginkgolic acids induce HepG2 cell death via a combination of apoptosis, autophagy and the mitochond...

Yang H, Wang C, Chen H, Li L, Ma S, Wang H, Fu Y, Qu T. Stem Cells Int. 2018 Apr 3;2018:4659159. doi: 10.1155/2018/4659159. eCollection 2018. Neural Stem Cell-Conditioned Medium Ameliorated Cerebral Ischemia-Reperfusion Injury in Rats

Feng D, Wu J, Tian Y, Zhou H, Zhou Y, Hu W, Zhao W, Wei H, Ling B, Ma C. Plos One. 2013 Nov 19;8(11):E80657. Doi: 10.1371/Journal.Pone.0080657. Ecollection 2013. Targeting Of Histone Deacetylases To Reactivate Tumour Suppressor Genes And Its Thera...

Du F, Ding Y, Zou J, Li Z, Tian J, She R, Wang D, Wang H, Lv D, Chang L. Plos One. 2015 May 22;10(5):E0127047. Doi: 10.1371/Journal.Pone.0127047. Ecollection 2015. Morphology And Molecular Mechanisms Of Hepatic Injury In Rats Under Simulated Weigh...

Li Tt, Zhang Jf, Fei Sj, Zhu Sp, Zhu Jz, Qiao X, Liu Zb. Acta Pharmacol Sin. 2014 Feb;35(2):185-94. Doi: 10.1038/Aps.2013.140. Epub 2013 Dec 23. Glutamate Microinjection Into The Hypothalamic Paraventricular Nucleus Attenuates Ulcerative Colitis I...

Chen Xl, Cao Lq, She Mr, Wang Q, Huang Xh, Fu Xh. World J Gastroenterol. 2008 Jan 28;14(4):582-9. Gli-1 Sirna Induced Apoptosis In Huh7 Cells.

Qin J, Liu M, Ding Q, Ji X, Hao Y, Wu X, Xiong J. Mol Cell Biochem. 2014 Oct;395(1-2):99-107. Doi: 10.1007/S11010-014-2115-2. Epub 2014 Jun 17. The Direct Effect Of Estrogen On Cell Viability And Apoptosis In Human Gastric Cancer Cells.

Lan Z, Liu J, Chen L, Fu Q, Luo J, Qu R, Kong L, Ma S. J Ethnopharmacol. 2012 May 7;141(1):386-95. Doi: 10.1016/J.Jep.2012.02.050. Epub 2012 Mar 5. Danggui-Shaoyao-San Ameliorates Cognition Deficits And Attenuates Oxidative Stress-Related Neuronal...

Sun W, Liu J, Huan Y, Zhang C. Inflamm Res. 2014 Apr;63(4):287-97. Doi: 10.1007/S00011-013-0699-8. Epub 2013 Dec 19. Intracranial Injection Of Recombinant Stromal-Derived Factor-1 Alpha (Sdf-1??) Attenuates Traumatic Brain Injury In Rats.

Yao K, Xing H, Yang W, Liao A, Wu B, Li Y, Zhang R, Liu Z. Tumour Biol. 2014 Aug;35(8):8023-31. Doi: 10.1007/S13277-014-2073-Z. Epub 2014 May 18. Knockdown Of Rlip76 Expression By Rna Interference Inhibits Proliferation, Enhances Apoptosis, And In...

Li X, Huo X, Zhang C, Ma X, Han F, Wang G. Cell Physiol Biochem. 2014;34(4):1227-40. Doi: 10.1159/000366334. Epub 2014 Sep 25. Role Of Continuous High Thoracic Epidural Anesthesia In Hippocampal Apoptosis After Global Cerebral Ischemia In Rats.

Gao H, Jiang Q, Han Y, Peng J, Wang C. Cell Biochem Biophys. 2015 Mar;71(2):827-35. Doi: 10.1007/S12013-014-0270-4. Shrna-Mediated Emmprin Silencing Inhibits Human Leukemic Monocyte Lymphoma U937 Cell Proliferation And Increases Chemosensitivity T...

Gao H, Jiang Q, Han Y, Peng J, Wang C. Cell Biochem Biophys. 2015 Mar;71(2):757-64. Doi: 10.1007/S12013-014-0260-6. Hispidulin Potentiates The Antitumor Effect Of Sunitinib Against Human Renal Cell Carcinoma In Laboratory Models.

Guan J, Li H, Lv T, Chen D, Yuan Y, Qu S. Biochem Biophys Res Commun. 2013 Nov 22;441(3):560-6. Doi: 10.1016/J.Bbrc.2013.10.121. Epub 2013 Oct 30. Bone Morphogenetic Protein-7 (Bmp-7) Mediates Ischemic Preconditioning-Induced Ischemic Tolerance Vi...

Gao H, Xie J, Peng J, Han Y, Jiang Q, Han M, Wang C. Exp Cell Res. 2015 Mar 15;332(2):236-46. Doi: 10.1016/J.Yexcr.2014.11.021. Epub 2014 Dec 8. Hispidulin Inhibits Proliferation And Enhances Chemosensitivity Of Gallbladder Cancer Cells By Targeti...

Zhou Pq, Fan H, Hu H, Tang Q, Liu Xx, Zhang Lj, Zhong M, Shou Zx. J Huazhong Univ Sci Technolog Med Sci. 2014 Dec;34(6):815-20. Doi: 10.1007/S11596-014-1358-1. Epub 2014 Dec 6. Role Of Dor-??-Arrestin1-Bcl2 Signal Transduction Pathway And Interven...

Tian L, Zhang J, Ge J, Xiao H, Lu J, Fu S, Liu M, Sun Y. Med Oncol. 2014 Jan;31(1):785. Doi: 10.1007/S12032-013-0785-3. Epub 2013 Dec 3. Microrna-205 Suppresses Proliferation And Promotes Apoptosis In Laryngeal Squamous Cell Carcinoma.

Rasul A, Khan M, Yu B, Ali M, Bo Yj, Yang H, Ma T. Arch Pharm Res. 2013 Oct;36(10):1262-9. Doi: 10.1007/S12272-013-0217-0. Epub 2013 Jul 24. Isoalantolactone, A Sesquiterpene Lactone, Induces Apoptosis In Sgc-7901 Cells Via Mitochondrial And Phosp...

Wang Py, Li Yj, Zhang S, Li Zl, Yue Z, Xie N, Xie Sy. Mol Cell Biochem. 2010 Jun;339(1-2):163-71. Doi: 10.1007/S11010-009-0380-2. Epub 2010 Jan 5. Regulating A549 Cells Growth By Aso Inhibiting Mirna Expression.

Li R, Wu X, Wei H, Tian S. Oncol Lett. 2013 Mar;5(3):877-883. Epub 2013 Jan 2. Characterization Of Side Population Cells Isolated From The Gastric Cancer Cell Line Sgc-7901.

Chen J, Wei H, Xie B, Wang B, Cheng J, Cheng J. Leuk Res. 2012 Dec;36(12):1526-35. Doi: 10.1016/J.Leukres.2012.08.018. Epub 2012 Sep 7. Endoplasmic Reticulum Stress Contributes To Arsenic Trioxide-Induced Apoptosis In Drug-Sensitive And -Resistant...

Chu Sh, Ma Yb, Feng Df, Zhang H, Qiu Jh, Zhu Za. Oncol Lett. 2011 Nov;2(6):1139-1142. Epub 2011 Aug 4. Elevated Expression Of Solute Carrier Family 22 Member 18 Increases The Sensitivity Of U251 Glioma Cells To Bcnu.

Zhao B, Li X. Oncol Rep. 2014 Jun;31(6):2769-75. Doi: 10.3892/Or.2014.3126. Epub 2014 Apr 3. Altholactone Induces Reactive Oxygen Species-Mediated Apoptosis In Bladder Cancer T24 Cells Through Mitochondrial Dysfunction, Mapk-P38 Activation And Akt...

Zhan W, Hu X, Yi J, An Q, Huang X. Mol Med Rep. 2015 Jun;11(6):4142-8. Doi: 10.3892/Mmr.2015.3326. Epub 2015 Feb 10. Inhibitory Activity Of Apogossypol In Human Prostate Cancer In Vitro And In Vivo.

Huang C, Lin Y, Su H, Ye D. Neurochem Res. 2015 Jan;40(1):27-35. Doi: 10.1007/S11064-014-1461-5. Epub 2014 Oct 25. Forsythiaside Protects Against Hydrogen Peroxide-Induced Oxidative Stress And Apoptosis In Pc12 Cell.

Chen Xm, Xie Xb, Zhao Q, Wang F, Bai Y, Yin Jq, Jiang H, Xie Xl, Jia Q, Huang G. Mol Med Rep. 2015 Jan;11(1):105-12. Doi: 10.3892/Mmr.2014.2733. Epub 2014 Oct 21. Ampelopsin Induces Apoptosis By Regulating Multiple C-Myc/S-Phase Kinase-Associated ...

Chen Xm, Bai Y, Zhong Yj, Xie Xl, Long Hw, Yang Yy, Wu Sg, Jia Q, Wang Xh. Plos One. 2013 Nov 12;8(11):E79201. Doi: 10.1371/Journal.Pone.0079201. Ecollection 2013. Wogonin Has Multiple Anti-Cancer Effects By Regulating C-Myc/Skp2/Fbw7?? And Hdac1/...

Liu Mw, Su Mx, Zhang W, Zhang Lm, Wang Yh, Qian Cy. Int J Mol Med. 2015 Aug;36(2):386-98. Doi: 10.3892/Ijmm.2015.2241. Epub 2015 Jun 10. Rhodiola Rosea Suppresses Thymus T-Lymphocyte Apoptosis By Downregulating Tumor Necrosis Factor-??-Induced Pro...

Chen P, Wang L, Li N, Liu Q, Ni J. Metallomics. 2013 May;5(5):541-50. Doi: 10.1039/C3Mt00002H. Epub 2013 Apr 16. Comparative Proteomics Analysis Of Sodium Selenite-Induced Apoptosis In Human Prostate Cancer Cells.

Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.

Sun Y, Xu Hj, Zhao Yx, Wang Lz, Sun Lr, Wang Z, Sun Xf. Evid Based Complement Alternat Med. 2013;2013:690164. Doi: 10.1155/2013/690164. Epub 2013 Mar 19. Crocin Exhibits Antitumor Effects On Human Leukemia Hl-60 Cells In Vitro And In Vivo.

Shuai H, Zhang J, Zhang J, Xie J, Zhang M, Yu Y, Zhang L. Endocr Res. 2011;36(1):25-34. Doi: 10.3109/07435800.2010.534753. Erythropoietin Protects Pancreatic ??-Cell Line Nit-1 Cells Against Cytokine-Induced Apoptosis Via Phosphatidylinositol 3-Ki...

Chen J, Huang Rb. Neurosciences (Riyadh). 2009 Oct;14(4):343-8. Protective Effect Of Yulangsan Polysaccharide On Focal Cerebral Ischemia/Reperfusion Injury In Rats And Its Underlying Mechanism.

Liu W, Zheng Q, Wang Y, Han X, Yuan L, Zhao M. Neurochem Res. 2015 Jan;40(1):70-80. Doi: 10.1007/S11064-014-1467-Z. Epub 2014 Nov 5. Transplantation Of Olfactory Ensheathing Cells Attenuates Acute Carbon Monoxide Poisoning-Induced Brain Damages In...

Yuan F, Dong P, Wang X, Fu X, Dai M, Zhang W. Exp Toxicol Pathol. 2013 Nov;65(7-8):1011-8. Doi: 10.1016/J.Etp.2013.02.004. Epub 2013 Mar 19. Toxicological Effects Of Cigarette Smoke On Ana-1 Macrophages In Vitro.

Luo Q, Li Z, Yan J, Zhu F, Xu Rj, Cai Yz. J Med Food. 2009 Aug;12(4):695-703. Doi: 10.1089/Jmf.2008.1232. Lycium Barbarum Polysaccharides Induce Apoptosis In Human Prostate Cancer Cells And Inhibits Prostate Cancer Growth In A Xenograft Mouse Mode...

Wu Cy, Tang Zh, Jiang L, Li Xf, Jiang Zs, Liu Ls. Mol Cell Biochem. 2012 Jan;359(1-2):347-58. Doi: 10.1007/S11010-011-1028-6. Epub 2011 Aug 17. Pcsk9 Sirna Inhibits Huvec Apoptosis Induced By Ox-Ldl Via Bcl/Bax-Caspase9-Caspase3 Pathway.

Rasul A, Yu B, Zhong L, Khan M, Yang H, Ma T. Oncol Rep. 2012 May;27(5):1481-7. Doi: 10.3892/Or.2012.1694. Epub 2012 Feb 21. Cytotoxic Effect Of Evodiamine In Sgc-7901 Human Gastric Adenocarcinoma Cells Via Simultaneous Induction Of Ap...

Li Y, Gu Wj, Liu Hl. Dig Dis Sci. 2012 Aug;57(8):2072-8. Doi: 10.1007/S10620-012-2132-5. Epub 2012 May 10. Induction Of Pancreatic Cancer Cell Apoptosis And Enhancement Of Gemcitabine Sensitivity By Rap80 Sirna.

Wang Y, Liu X, Zhang D, Chen J, Liu S, Berk M. Bmc Med. 2013 Feb 8;11:32. Doi: 10.1186/1741-7015-11-32. The Effects Of Apoptosis Vulnerability Markers On The Myocardium In Depression After Myocardial Infarction.

Gu Wj, Liu Hl. Anticancer Drugs. 2013 Jul;24(6):566-76. Doi: 10.1097/Cad.0B013E3283608Bc5. Induction Of Pancreatic Cancer Cell Apoptosis, Invasion, Migration, And Enhancement Of Chemotherapy Sensitivity Of Gemcitabine, 5-Fu, And Oxaliplatin By Hnr...

Wu K, Liu Y, Lv Y, Cui L, Li W, Chen J, Liang Nc, Li L. Oncol Rep. 2013 Jun;29(6):2101-8. Doi: 10.3892/Or.2013.2375. Epub 2013 Apr 3. Ent-11??-Hydroxy-15-Oxo-Kaur-16-En-19-Oic-Acid Induces Apoptosis And Cell Cycle Arrest In Cne-2Z Nasopharyngeal C...

Qin R, Shen H, Cao Y, Fang Y, Li H, Chen Q, Xu W. Plos One. 2013 Oct 1;8(10):E76486. Doi: 10.1371/Journal.Pone.0076486. Ecollection 2013. Tetrandrine Induces Mitochondria-Mediated Apoptosis In Human Gastric Cancer Bgc-823 Cells.

Wei T, Tian W, Liu F, Xie G. Biochem Biophys Res Commun. 2014 May 16;447(4):666-71. Doi: 10.1016/J.Bbrc.2014.04.074. Epub 2014 Apr 19. Protective Effects Of Exogenous ??-Hydroxybutyrate On Paraquat Toxicity In Rat Kidney.

Wang Y, Zhang H, Chai F, Liu X, Berk M,,,,. Bmc Psychiatry. 2014 Dec 4;14:349. Doi: 10.1186/S12888-014-0349-X. The Effects Of Escitalopram On Myocardial Apoptosis And The Expression Of Bax And Bcl-2 During Myocardial Ischemia/Reperfusion In A Mode...

Wu C, Dong S, Li Y. Int J Mol Med. 2015 Apr;35(4):893-900. Doi: 10.3892/Ijmm.2015.2105. Epub 2015 Feb 18. Effects Of Mirna-455 On Cardiac Hypertrophy Induced By Pressure Overload.

Han N, Yu L, Song Z, Luo L, Wu Y. Mol Med Rep. 2015 Jul;12(1):1098-106. Doi: 10.3892/Mmr.2015.3540. Epub 2015 Mar 24. Agmatine Protects M??ller Cells From High-Concentration Glucose-Induced Cell Damage Via N-Methyl-D-Aspartic Acid Receptor Inhibit...

Sun J, Zhang H, Guan L, Zhou H, Sun M. Int J Clin Exp Med. 2015 Jan 15;8(1):358-67. Ecollection 2015. Alpha-Lipoic Acid Attenuates Trinitrobenzene Sulfonic Acid-Induced Ulcerative Colitis In Mice.

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16 Customer Q&As for Anti-Bcl2 alpha Antibody

Question

Is a blocking peptide available for product anti-Bcl2 alpha antibody (RP1003)?

Verified Customer

Verified customer

Asked: 2020-03-10

Answer

We do provide the blocking peptide for product anti-Bcl2 alpha antibody (RP1003). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-10

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thyroid gland using anti-Bcl2 alpha antibody RP1003. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-16

Question

Do you have a BSA free version of anti-Bcl2 alpha antibody RP1003 available?

Verified Customer

Verified customer

Asked: 2019-09-10

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bcl2 alpha antibody RP1003 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-10

Question

Is this RP1003 anti-Bcl2 alpha antibody reactive to the isotypes of BCL2?

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

The immunogen of RP1003 anti-Bcl2 alpha antibody is E. coli-derived human BCL-2 recombinant protein(Position: M1-D211). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-23

Question

See below the WB image, lot number and protocol we used for thyroid gland using anti-Bcl2 alpha antibody RP1003. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-22

Question

I have a question about product RP1003, anti-Bcl2 alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1003 anti-Bcl2 alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-09

Question

We need to test anti-Bcl2 alpha antibody RP1003 on human thyroid gland for research purposes, then I may be interested in using anti-Bcl2 alpha antibody RP1003 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-05-09

Answer

The products we sell, including anti-Bcl2 alpha antibody RP1003, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-09

Question

I see that the anti-Bcl2 alpha antibody RP1003 works with ELISA, what is the protocol used to produce the result images on the product page?

S. Collins

Verified customer

Asked: 2018-11-26

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-11-26

Question

Will RP1003 anti-Bcl2 alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-11-23

Answer

As indicated on the product datasheet, RP1003 anti-Bcl2 alpha antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-11-23

Question

We have seen staining in human thyroid gland. Any tips? Is anti-Bcl2 alpha antibody supposed to stain thyroid gland positively?

Verified Customer

Verified customer

Asked: 2018-09-28

Answer

From literature thyroid gland does express BCL2. From Uniprot.org, BCL2 is expressed in thyroid gland, testis, among other tissues. Regarding which tissues have BCL2 expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-09-28

Question

I was wanting to use your anti-Bcl2 alpha antibody for ELISA for human thyroid gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human thyroid gland identification?

Verified Customer

Verified customer

Asked: 2017-10-27

Answer

It shows on the product datasheet, RP1003 anti-Bcl2 alpha antibody has been tested for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human thyroid gland in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-27

Question

We are currently using anti-Bcl2 alpha antibody RP1003 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2017-06-08

Answer

The anti-Bcl2 alpha antibody (RP1003) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-08

Question

Will anti-Bcl2 alpha antibody RP1003 work on primate WB with thyroid gland?

Z. Carter

Verified customer

Asked: 2015-07-27

Answer

Our lab technicians have not tested anti-Bcl2 alpha antibody RP1003 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Bcl2 alpha antibody RP1003 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate thyroid gland in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-07-27

Question

Does anti-Bcl2 alpha antibody RP1003 work for ELISA with thyroid gland?

S. Mitchell

Verified customer

Asked: 2015-03-24

Answer

According to the expression profile of thyroid gland, BCL2 is highly expressed in thyroid gland. So, it is likely that anti-Bcl2 alpha antibody RP1003 will work for ELISA with thyroid gland.

Boster Scientific Support

Answered: 2015-03-24

Question

My boss were content with the WB result of your anti-Bcl2 alpha antibody. However we have seen positive staining in testis mitochondrion outer membrane using this antibody. Is that expected? Could you tell me where is BCL2 supposed to be expressed?

J. Zhao

Verified customer

Asked: 2014-12-09

Answer

According to literature, testis does express BCL2. Generally BCL2 expresses in mitochondrion outer membrane. Regarding which tissues have BCL2 expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2014-12-09

Question

Our lab used your anti-Bcl2 alpha antibody for ELISA on thyroid gland last year. I am using human, and We are going to use the antibody for WB next. We want examining thyroid gland as well as testis in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

A. Zhao

Verified customer

Asked: 2013-11-19

Answer

I have checked the website and datasheets of our anti-Bcl2 alpha antibody and I see that RP1003 has been tested on human in both ELISA and WB. Thus RP1003 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-11-19

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RP1003
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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