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Product Info Summary
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Anti-Bcl2 alpha Antibody
Boster Bio Anti-Bcl2 alpha Antibody catalog # RP1003. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bcl2 alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1003)
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
E. coli-derived human BCL-2 recombinant protein (Position: M1-D211).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross-reactivity with other proteins
RP1003 is reactive to BCL2 in Human
RP1003 is guaranteed for ELISA, WB Boster Guarantee
Background of Bcl-2
Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t (14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure. Western blot analysis of BCL-2 using anti-BCL-2 antibody (RP1003).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane : Recombinant Human Bcl-2 Protein 0.5ng,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL-2 antigen affinity purified polyclonal antibody (Catalog # RP1003) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL-2 at approximately 26KD. The expected band size for BCL-2 is at 26KD.
Gene/Protein Information For BCL2 (Source: Uniprot.org, NCBI)
Apoptosis regulator Bcl-2
apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl2; Bcl-2 BCL2 Bcl-2, PPP1R50 BCL2 apoptosis regulator apoptosis regulator Bcl-2|B-cell CLL/lymphoma 2|protein phosphatase 1, regulatory subunit 50*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on BCL2, check out the BCL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
RP1003 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Scutellarein selectively targets multiple myeloma cells by increasing mitochondrial superoxide production and activating intrinsic apoptosis pathway
Long J,Liu Z,Hui L.Anti-tumor effect and mechanistic study of elemene on pancreatic carcinoma.BMC Complement Altern Med.2019 Jun 18;19(1):133.doi: 10.1186/s12906-019-2544-2.PMID:31215421;PMCID:PMC6582541.
RP1003 usage in article: APP:WB, SAMPLE:PANCREATIC TISSUE, DILUTION:1:500
Chen M,Wang Z,Zhou W,Lu C,Ji T,Yang W,Jin Z,Tian Y,Lei W,Wu S,Fu Q,Wu Z,Wu X,Han M,Fang M,Yang Y.SIRT1/PGC-1α signaling activation by mangiferin attenuates cerebral hypoxia/reoxygenation injury in neuroblastoma cells.Eur J Pharmacol.2021 Jun 8:174236.doi:10.1016/j.ejphar.2021.174236.Epub ahead of print.PMID:34116043.
RP1003 usage in article: APP:WB, SAMPLE:N2A CELL, DILUTION:1:500
Junyi Tang,Kehua Fang,Chang Li,Xiaotian Chang,"ARHGEF10L Promotes Cervical Tumorigenesis via RhoA-Mediated Signaling",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 6683264,11 pages,2021.https://doi.org/10.1155/2021/6683264
RP1003 usage in article: APP:WB, SAMPLE:HELA CELL, DILUTION:NA
Zhang Q,Duan HX,Li RL,Sun JY,Liu J,Peng W,Wu CJ,Gao YX. Inducing Apoptosis and Suppressing Inflammatory Reactions in Synovial Fibroblasts are Two Important Ways for Guizhi-Shaoyao-Zhimu Decoction Against Rheumatoid Arthritis. J Inflamm Res.2021 Jan 26;14:
RP1003 usage in article: APP:WB, SAMPLE:MH7A CELL, DILUTION:1:300
Cao X,Fang X,Malik WS,He Y,Li X,Xie M,Sun W,Xu Y,Liu X. TRB3 interacts with ERK and JNK and contributes to the proliferation, apoptosis, and migration of lung adenocarcinoma cells. J Cell Physiol.2020 Jan;235(1): 538-547.doi:10.1002/jcp.28993.Epub 2019 Ju
RP1003 usage in article: APP:WB, SAMPLE:LUNG TISSUE,A549 CELL AND H1299 CELL, DILUTION:NA
Liu L,Liu F,Sun Z,Peng Z,You T,Yu Z. LncRNA NEAT1 promotes apoptosis and inflammation in LPS-induced sepsis models by targeting miR-590-3p. Exp Ther Med.2020 Oct; 20(4):3290-3300. doi:10.3892/etm.2020.9079. Epub 2020 Jul 29.PMID:32855700;PMCID:PMC7444425.
RP1003 usage in article: APP:WB, SAMPLE: H9C2 CELL, DILUTION:1:500
Chen H,Sheng H,Zhao Y,Zhu G.Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway.Med Sci Monit.2020 Dec 31;26:e928403.doi:10.12659/MSM.928403.PMID:33382
Oleanolic acid from Prunella Vulgaris L. Induces SPC-A-1 cell line apoptosis via regulation of Bax, Bad and Bcl-2 Expression
Baicalin attenuates alcoholic liver injury through modulation of hepatic oxidative stress, inflammation and sonic hedgehog pathway in rats
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