SKU RP1003
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications ELISA, WB

Overview

Product Name Anti-Bcl2 alpha Antibody
SKU/Catalog Number RP1003
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Apoptosis regulator Bcl-2(BCL2) detection. Tested with WB, ELISA in Human.
Cite This Product Anti-Bcl2 alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1003)
Host Rabbit
Immunogen E. coli-derived human BCL-2 recombinant protein(Position: M1-D211).
Reactivity Human

Assay Details

Assay Dilutions Overview

ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

/antibody/rp1003 1 WB anti human bcl 2 antibody.jpg

Figure. Western blot analysis of BCL-2 using anti- BCL-2 antibody (RP1003).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.

Lane : Recombinant Human Bcl-2 Protein 0.5ng,

After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- BCL-2 antigen affinity purified polyclonal antibody (Catalog # RP1003) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL-2 at approximately 26KD. The expected band size for BCL-2 is at 26KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P10415
Gene Name BCL2
Protein Name Apoptosis regulator Bcl-2
Tissue Specificity Expressed in a variety of tissues.
Alternative Names Apoptosis regulator Bcl-2;BCL2;
Subcellular Localization Mitochondrion outer membrane ; Single-pass membrane protein . Nucleus membrane ; Single-pass membrane protein . Endoplasmic reticulum membrane ; Single-pass membrane protein .
Molecular Weight 26266 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). .
Background Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

Order Product (RP1003)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1054 $240
100ug+Free Biotin Secondary BA1003 $240

USD $240

Ships in 5-7 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Write a review for RP1003

Publications

Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats
Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3-E1 cells
N-acetylcysteine reduces oxidative stress, nuclear factor-?B activity and cardiomyocyte apoptosis in heart failure
Fibroblast growth factor 21 protects rat cardiomyocytes from endoplasmic reticulum stress by promoting the fibroblast growth factor receptor 1-extracellular signal-regulated kinase 1/2 signaling pathway
Ailanthone induces G2/M cell cycle arrest and apoptosis of SGC-7901 human gastric cancer cells
1-calcium phosphate-uracil, a synthesized pyrimidine derivative agent, has anti-proliferative, pro-apoptotic and anti-invasion effects on multiple tumor cell lines
Ginkgolic acids induce HepG2 cell death via a combination of apoptosis, autophagy and the mitochondrial pathway
CCAAT/enhancer binding protein homologous protein knockdown alleviates hypoxia-induced myocardial injury in rat cardiomyocytes exposed to high glucose
Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian Granular Cells of Mice
Ginsenoside Rb1 inhibit apoptosis in rat model of Alzheimer?s disease induced by A?1-40
Hepatitis E Virus Induces Hepatocyte Apoptosis via Mitochondrial Pathway in Mongolian Gerbils
Effects of 5-hydroxy-4?-nitro-7-propionyloxy-genistein on inhibiting proliferation and invasion via activating reactive oxygen species in human ovarian cancer A2780/DDP cells
Downregulation of BRAF-activated non-coding RNA suppresses the proliferation, migration and invasion, and induces apoptosis of hepatocellular carcinoma cells
Blockade of sonic hedgehog signaling decreases viability and induces apoptosis in retinoblastoma cells: The key role of the PI3K/Akt pathway
Siva 1 inhibits proliferation, migration and invasion by phosphorylating Stathmin in ovarian cancer cells
RKTG overexpression inhibits proliferation and induces apoptosis of human leukemia cells via suppression of the ERK and PI3K/AKT signaling pathways
Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats
BMP-7 suppresses excessive scar formation by activating the BMP-7/Smad1/5/8 signaling pathway
Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure
Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells
Cardioprotective effects of traditional Chinese medicine Guanmaitong on acute myocardial infarction
Vanadium toxicity in the thymic development
Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells
Comparative Study on the Protective Effects of Salidroside and Hypoxic Preconditioning for Attenuating Anoxia-Induced Apoptosis in Pheochromocytoma (PC12) Cells
Knockdown of High Mobility Group-Box 3 (HMGB3) Expression Inhibits Proliferation, Reduces Migration, and Affects Chemosensitivity in Gastric Cancer Cells
Valproate Attenuates Nitroglycerin-Induced Trigeminovascular Activation by Preserving Mitochondrial Function in a Rat Model of Migraine
Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis
A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway
The Effects of Fasudil at Different Doses on Acute Myocardial Infarction in Rats
Radix Ilicis Pubescentis total flavonoids combined with mobilization of bone marrow stem cells to protect against cerebral ischemia/reperfusion injury
Astaxanthin Inhibits Acetaldehyde-Induced Cytotoxicity in SH-SY5Y Cells by Modulating Akt/CREB and p38MAPK/ERK Signaling Pathways
Nek7 is overexpressed in hepatocellular carcinoma and promotes hepatocellular carcinoma cell proliferation in vitro and in vivo
Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-?B Signaling Pathway
Mesenchymal stem cells promote colorectal cancer progression through AMPK/mTOR-mediated NF-?B activation
Yangjing Capsule Ameliorates Spermatogenesis in Male Mice Exposed to Cyclophosphamide
Effects of As2O3nanoparticles on cell growth and apoptosis of NB4 cells
Fenofibrate, a PPAR? agonist, protect proximal tubular cells from albumin-bound fatty acids induced apoptosis via the activation of NF-kB
Hippocampal mitogen-activated protein kinase activation is associated with intermittent hypoxia in a rat model of obstructive sleep apnea syndrome
Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats
Effect of curcumin on Bcl-2 and Bax expression in nude mice prostate cancer.
Expression of TP53, BCL-2, and VEGFA Genes in Esophagus Carcinoma and its Biological Significance
Adipose-Derived Mesenchymal Stem Cells Transplantation Alleviates Renal Injury in Streptozotocin-Induced Diabetic Nephropathy
Effects of intracerebral hemorrhage and subsequent minimally invasive hematoma aspiration on expression of apoptosisrelated genes in rats.
A preliminary study of the effect of ECRG4 overexpression on the proliferation and apoptosis of human laryngeal cancer cells and the underlying mechanisms
Afatinib inhibits proliferation and invasion and promotes apoptosis of the T24 bladder cancer cell line
Effects of PDTC on NF-?B expression and apoptosis in rats with severe acute pancreatitis-associated lung injury
Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-?-induced protein 8-like-2 in septic rats
Morphology and Molecular Mechanisms of Hepatic Injury in Rats under Simulated Weightlessness and the Protective Effects of Resistance Training
Agmatine protects M?ller cells from high-concentration glucose-induced cell damage via N-methyl-D-aspartic acid receptor inhibition
Alpha-lipoic acid attenuates trinitrobenzene sulfonic acid-induced ulcerative colitis in mice
Effects of miRNA-455 on cardiac hypertrophy induced by pressure overload
Apoptosis-related protein expression in rabbits with blast brain injury following early hyperbaric oxygen therapy
Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application
The Viral Oncoprotein HBx of Hepatitis B virus Promotes the Growth of Hepatocellular Carcinoma through Cooperating with the Cellular Oncoprotein RMP
The effects of escitalopram on myocardial apoptosis and the expression of Bax and Bcl-2 during myocardial ischemia/reperfusion in a model of rats with depression
Platycodin D Induces Tumor Growth Arrest by Activating FOXO3a Expression in Prostate Cancerin vitroandin vivo
Platycodin D Induces Tumor Growth Arrest by Activating FOXO3a Expression in Prostate Cancer in vitro and in vivo
Ampelopsin induces apoptosis by regulating multiple c-Myc/S-phase kinase-associated protein 2/F-box and WD repeat-containing protein 7/histone deacetylase 2 pathways in human lung adenocarcinoma cells
Acupuncture atBaihuiandDazhuireduces brain cell apoptosis in heroin readdicts
8-hydroxy-2-(di-n-propylamino)tetralin intervenes with neural cell apoptosis following diffuse axonal injury
Effect of ligustilide on Ang II-induced hypertrophy in cardiomyocytes and the potential mechanisms
Antitumor effects of artesunate on human breast carcinoma MCF-7 cells and IGF-IR expression in nude mice xenografts
Pathway of Programmed Cell Death and Oxidative Stress Induced by ?-Hydroxybutyrate in Dairy Cow Abomasum Smooth Muscle Cells and in Mouse Gastric Smooth Muscle
Glutamate microinjection into the hypothalamic paraventricular nucleus attenuates ulcerative colitis in rats
Wogonin Has Multiple Anti-Cancer Effects by Regulating c-Myc/SKP2/Fbw7? and HDAC1/HDAC2 Pathways and Inducing Apoptosis in Human Lung Adenocarcinoma Cell Line A549
Targeting of Histone Deacetylases to Reactivate Tumour Suppressor Genes and Its Therapeutic Potential in a Human Cervical Cancer Xenograft Model
Tetrandrine Induces Mitochondria-Mediated Apoptosis in Human Gastric Cancer BGC-823 Cells
RMP Plays Distinct Roles in the Proliferation of Hepatocellular Carcinoma Cells and Normal Hepatic Cells
Delivery of the co-expression plasmid pEndo-Si-Stat3 by attenuatedSalmonellaserovartyphimuriumfor prostate cancer treatment
Characterization of side population cells isolated from the gastric cancer cell line SGC-7901
Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury
Does dynamic immobilization reduce chondrocyte apoptosis and disturbance to the femoral head perfusion?
Magnolol, a natural compound, induces apoptosis of SGC-7901 human gastric adenocarcinoma cells via the mitochondrial and PI3K/Akt signaling pathways
Iptakalim, an ATP-sensitive potassium channel opener, confers neuroprotection against cerebral ischemia/reperfusion injury in rats by protecting neurovascular unit cells
RPB5-mediating Protein Is Required for the Proliferation of Hepatocellular Carcinoma Cells
Expression of Survivin in pancreatic cancer and its correlation to expression of Bcl-2
In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures
Gli-1 siRNA induced apoptosis in Huh7 cells
Effects of garlicin on apoptosis in rat model of colitis
Zhi Cui, Ye Cui, Shuting Yang, Gan Luo, Yang Wang, Yixin Lou, Xinhua Sun DOI: 10.1002/cbin.10736 KLK4 silencing inhibits the growth of oral squamous cell carcinoma through Wnt/β-catenin signaling pathway
Qin J, Liu M, Ding Q, Ji X, Hao Y, Wu X, Xiong J. Mol Cell Biochem. 2014 Oct;395(1-2):99-107. Doi: 10.1007/S11010-014-2115-2. Epub 2014 Jun 17. The Direct Effect Of Estrogen On Cell Viability And Apoptosis In Human Gastric Cancer Cells.
Lan Z, Liu J, Chen L, Fu Q, Luo J, Qu R, Kong L, Ma S. J Ethnopharmacol. 2012 May 7;141(1):386-95. Doi: 10.1016/J.Jep.2012.02.050. Epub 2012 Mar 5. Danggui-Shaoyao-San Ameliorates Cognition Deficits And Attenuates Oxidative Stress-Related Neuronal...
Sun W, Liu J, Huan Y, Zhang C. Inflamm Res. 2014 Apr;63(4):287-97. Doi: 10.1007/S00011-013-0699-8. Epub 2013 Dec 19. Intracranial Injection Of Recombinant Stromal-Derived Factor-1 Alpha (Sdf-1??) Attenuates Traumatic Brain Injury In Rats.
Yao K, Xing H, Yang W, Liao A, Wu B, Li Y, Zhang R, Liu Z. Tumour Biol. 2014 Aug;35(8):8023-31. Doi: 10.1007/S13277-014-2073-Z. Epub 2014 May 18. Knockdown Of Rlip76 Expression By Rna Interference Inhibits Proliferation, Enhances Apoptosis, And In...
Li X, Huo X, Zhang C, Ma X, Han F, Wang G. Cell Physiol Biochem. 2014;34(4):1227-40. Doi: 10.1159/000366334. Epub 2014 Sep 25. Role Of Continuous High Thoracic Epidural Anesthesia In Hippocampal Apoptosis After Global Cerebral Ischemia In Rats.
Gao H, Jiang Q, Han Y, Peng J, Wang C. Cell Biochem Biophys. 2015 Mar;71(2):827-35. Doi: 10.1007/S12013-014-0270-4. Shrna-Mediated Emmprin Silencing Inhibits Human Leukemic Monocyte Lymphoma U937 Cell Proliferation And Increases Chemosensitivity T...
Gao H, Jiang Q, Han Y, Peng J, Wang C. Cell Biochem Biophys. 2015 Mar;71(2):757-64. Doi: 10.1007/S12013-014-0260-6. Hispidulin Potentiates The Antitumor Effect Of Sunitinib Against Human Renal Cell Carcinoma In Laboratory Models.
Guan J, Li H, Lv T, Chen D, Yuan Y, Qu S. Biochem Biophys Res Commun. 2013 Nov 22;441(3):560-6. Doi: 10.1016/J.Bbrc.2013.10.121. Epub 2013 Oct 30. Bone Morphogenetic Protein-7 (Bmp-7) Mediates Ischemic Preconditioning-Induced Ischemic Tolerance Vi...
Gao H, Xie J, Peng J, Han Y, Jiang Q, Han M, Wang C. Exp Cell Res. 2015 Mar 15;332(2):236-46. Doi: 10.1016/J.Yexcr.2014.11.021. Epub 2014 Dec 8. Hispidulin Inhibits Proliferation And Enhances Chemosensitivity Of Gallbladder Cancer Cells By Targeti...
Zhou Pq, Fan H, Hu H, Tang Q, Liu Xx, Zhang Lj, Zhong M, Shou Zx. J Huazhong Univ Sci Technolog Med Sci. 2014 Dec;34(6):815-20. Doi: 10.1007/S11596-014-1358-1. Epub 2014 Dec 6. Role Of Dor-??-Arrestin1-Bcl2 Signal Transduction Pathway And Intervent...
Tian L, Zhang J, Ge J, Xiao H, Lu J, Fu S, Liu M, Sun Y. Med Oncol. 2014 Jan;31(1):785. Doi: 10.1007/S12032-013-0785-3. Epub 2013 Dec 3. Microrna-205 Suppresses Proliferation And Promotes Apoptosis In Laryngeal Squamous Cell Carcinoma.
Rasul A, Khan M, Yu B, Ali M, Bo Yj, Yang H, Ma T. Arch Pharm Res. 2013 Oct;36(10):1262-9. Doi: 10.1007/S12272-013-0217-0. Epub 2013 Jul 24. Isoalantolactone, A Sesquiterpene Lactone, Induces Apoptosis In Sgc-7901 Cells Via Mitochondrial And Phosp...
Wang Py, Li Yj, Zhang S, Li Zl, Yue Z, Xie N, Xie Sy. Mol Cell Biochem. 2010 Jun;339(1-2):163-71. Doi: 10.1007/S11010-009-0380-2. Epub 2010 Jan 5. Regulating A549 Cells Growth By Aso Inhibiting Mirna Expression.
Li R, Wu X, Wei H, Tian S. Oncol Lett. 2013 Mar;5(3):877-883. Epub 2013 Jan 2. Characterization Of Side Population Cells Isolated From The Gastric Cancer Cell Line Sgc-7901.
Chen J, Wei H, Xie B, Wang B, Cheng J, Cheng J. Leuk Res. 2012 Dec;36(12):1526-35. Doi: 10.1016/J.Leukres.2012.08.018. Epub 2012 Sep 7. Endoplasmic Reticulum Stress Contributes To Arsenic Trioxide-Induced Apoptosis In Drug-Sensitive And -Resistant...
Chu Sh, Ma Yb, Feng Df, Zhang H, Qiu Jh, Zhu Za. Oncol Lett. 2011 Nov;2(6):1139-1142. Epub 2011 Aug 4. Elevated Expression Of Solute Carrier Family 22 Member 18 Increases The Sensitivity Of U251 Glioma Cells To Bcnu.
Zhao B, Li X. Oncol Rep. 2014 Jun;31(6):2769-75. Doi: 10.3892/Or.2014.3126. Epub 2014 Apr 3. Altholactone Induces Reactive Oxygen Species-Mediated Apoptosis In Bladder Cancer T24 Cells Through Mitochondrial Dysfunction, Mapk-P38 Activation And Akt...
Zhan W, Hu X, Yi J, An Q, Huang X. Mol Med Rep. 2015 Jun;11(6):4142-8. Doi: 10.3892/Mmr.2015.3326. Epub 2015 Feb 10. Inhibitory Activity Of Apogossypol In Human Prostate Cancer In Vitro And In Vivo.
Huang C, Lin Y, Su H, Ye D. Neurochem Res. 2015 Jan;40(1):27-35. Doi: 10.1007/S11064-014-1461-5. Epub 2014 Oct 25. Forsythiaside Protects Against Hydrogen Peroxide-Induced Oxidative Stress And Apoptosis In Pc12 Cell.
Chen Xm, Xie Xb, Zhao Q, Wang F, Bai Y, Yin Jq, Jiang H, Xie Xl, Jia Q, Huang G. Mol Med Rep. 2015 Jan;11(1):105-12. Doi: 10.3892/Mmr.2014.2733. Epub 2014 Oct 21. Ampelopsin Induces Apoptosis By Regulating Multiple C-Myc/S-Phase Kinase-Associated ...
Chen Xm, Bai Y, Zhong Yj, Xie Xl, Long Hw, Yang Yy, Wu Sg, Jia Q, Wang Xh. Plos One. 2013 Nov 12;8(11):E79201. Doi: 10.1371/Journal.Pone.0079201. Ecollection 2013. Wogonin Has Multiple Anti-Cancer Effects By Regulating C-Myc/Skp2/Fbw7?? And Hdac1/H...
Liu Mw, Su Mx, Zhang W, Zhang Lm, Wang Yh, Qian Cy. Int J Mol Med. 2015 Aug;36(2):386-98. Doi: 10.3892/Ijmm.2015.2241. Epub 2015 Jun 10. Rhodiola Rosea Suppresses Thymus T-Lymphocyte Apoptosis By Downregulating Tumor Necrosis Factor-??-Induced Prot...
Chen P, Wang L, Li N, Liu Q, Ni J. Metallomics. 2013 May;5(5):541-50. Doi: 10.1039/C3Mt00002H. Epub 2013 Apr 16. Comparative Proteomics Analysis Of Sodium Selenite-Induced Apoptosis In Human Prostate Cancer Cells.
Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.
Sun Y, Xu Hj, Zhao Yx, Wang Lz, Sun Lr, Wang Z, Sun Xf. Evid Based Complement Alternat Med. 2013;2013:690164. Doi: 10.1155/2013/690164. Epub 2013 Mar 19. Crocin Exhibits Antitumor Effects On Human Leukemia Hl-60 Cells In Vitro And In Vivo.
Shuai H, Zhang J, Zhang J, Xie J, Zhang M, Yu Y, Zhang L. Endocr Res. 2011;36(1):25-34. Doi: 10.3109/07435800.2010.534753. Erythropoietin Protects Pancreatic ??-Cell Line Nit-1 Cells Against Cytokine-Induced Apoptosis Via Phosphatidylinositol 3-Kin...
Chen J, Huang Rb. Neurosciences (Riyadh). 2009 Oct;14(4):343-8. Protective Effect Of Yulangsan Polysaccharide On Focal Cerebral Ischemia/Reperfusion Injury In Rats And Its Underlying Mechanism.
Liu W, Zheng Q, Wang Y, Han X, Yuan L, Zhao M. Neurochem Res. 2015 Jan;40(1):70-80. Doi: 10.1007/S11064-014-1467-Z. Epub 2014 Nov 5. Transplantation Of Olfactory Ensheathing Cells Attenuates Acute Carbon Monoxide Poisoning-Induced Brain Damages In...
Yuan F, Dong P, Wang X, Fu X, Dai M, Zhang W. Exp Toxicol Pathol. 2013 Nov;65(7-8):1011-8. Doi: 10.1016/J.Etp.2013.02.004. Epub 2013 Mar 19. Toxicological Effects Of Cigarette Smoke On Ana-1 Macrophages In Vitro.
Luo Q, Li Z, Yan J, Zhu F, Xu Rj, Cai Yz. J Med Food. 2009 Aug;12(4):695-703. Doi: 10.1089/Jmf.2008.1232. Lycium Barbarum Polysaccharides Induce Apoptosis In Human Prostate Cancer Cells And Inhibits Prostate Cancer Growth In A Xenograft Mouse Mode...
Wu Cy, Tang Zh, Jiang L, Li Xf, Jiang Zs, Liu Ls. Mol Cell Biochem. 2012 Jan;359(1-2):347-58. Doi: 10.1007/S11010-011-1028-6. Epub 2011 Aug 17. Pcsk9 Sirna Inhibits Huvec Apoptosis Induced By Ox-Ldl Via Bcl/Bax-Caspase9-Caspase3 Pathway.
Rasul A, Yu B, Zhong L, Khan M, Yang H, Ma T. Oncol Rep. 2012 May;27(5):1481-7. Doi: 10.3892/Or.2012.1694. Epub 2012 Feb 21. Cytotoxic Effect Of Evodiamine In Sgc-7901 Human Gastric Adenocarcinoma Cells Via Simultaneous Induction Of Ap...
Li Y, Gu Wj, Liu Hl. Dig Dis Sci. 2012 Aug;57(8):2072-8. Doi: 10.1007/S10620-012-2132-5. Epub 2012 May 10. Induction Of Pancreatic Cancer Cell Apoptosis And Enhancement Of Gemcitabine Sensitivity By Rap80 Sirna.
Wang Y, Liu X, Zhang D, Chen J, Liu S, Berk M. Bmc Med. 2013 Feb 8;11:32. Doi: 10.1186/1741-7015-11-32. The Effects Of Apoptosis Vulnerability Markers On The Myocardium In Depression After Myocardial Infarction.
Gu Wj, Liu Hl. Anticancer Drugs. 2013 Jul;24(6):566-76. Doi: 10.1097/Cad.0B013E3283608Bc5. Induction Of Pancreatic Cancer Cell Apoptosis, Invasion, Migration, And Enhancement Of Chemotherapy Sensitivity Of Gemcitabine, 5-Fu, And Oxaliplatin By Hnr...
Wu K, Liu Y, Lv Y, Cui L, Li W, Chen J, Liang Nc, Li L. Oncol Rep. 2013 Jun;29(6):2101-8. Doi: 10.3892/Or.2013.2375. Epub 2013 Apr 3. Ent-11??-Hydroxy-15-Oxo-Kaur-16-En-19-Oic-Acid Induces Apoptosis And Cell Cycle Arrest In Cne-2Z Nasopharyngeal Ca...
Qin R, Shen H, Cao Y, Fang Y, Li H, Chen Q, Xu W. Plos One. 2013 Oct 1;8(10):E76486. Doi: 10.1371/Journal.Pone.0076486. Ecollection 2013. Tetrandrine Induces Mitochondria-Mediated Apoptosis In Human Gastric Cancer Bgc-823 Cells.
Wei T, Tian W, Liu F, Xie G. Biochem Biophys Res Commun. 2014 May 16;447(4):666-71. Doi: 10.1016/J.Bbrc.2014.04.074. Epub 2014 Apr 19. Protective Effects Of Exogenous ??-Hydroxybutyrate On Paraquat Toxicity In Rat Kidney.
Wang Y, Zhang H, Chai F, Liu X, Berk M,,,,. Bmc Psychiatry. 2014 Dec 4;14:349. Doi: 10.1186/S12888-014-0349-X. The Effects Of Escitalopram On Myocardial Apoptosis And The Expression Of Bax And Bcl-2 During Myocardial Ischemia/Reperfusion In A Mode...
Wu C, Dong S, Li Y. Int J Mol Med. 2015 Apr;35(4):893-900. Doi: 10.3892/Ijmm.2015.2105. Epub 2015 Feb 18. Effects Of Mirna-455 On Cardiac Hypertrophy Induced By Pressure Overload.
Han N, Yu L, Song Z, Luo L, Wu Y. Mol Med Rep. 2015 Jul;12(1):1098-106. Doi: 10.3892/Mmr.2015.3540. Epub 2015 Mar 24. Agmatine Protects M??ller Cells From High-Concentration Glucose-Induced Cell Damage Via N-Methyl-D-Aspartic Acid Receptor Inhibition.
Sun J, Zhang H, Guan L, Zhou H, Sun M. Int J Clin Exp Med. 2015 Jan 15;8(1):358-67. Ecollection 2015. Alpha-Lipoic Acid Attenuates Trinitrobenzene Sulfonic Acid-Induced Ulcerative Colitis In Mice.
Rasul A, Ding C, Li X, Khan M, Yi F, Ali M, Ma T. Apoptosis. 2012 Oct;17(10):1104-19. Doi: 10.1007/S10495-012-0742-1. Dracorhodin Perchlorate Inhibits Pi3K/Akt And Nf-??b Activation, Up-Regulates The Expression Of P53, And Enhances Apoptosis.
Yin W, Li B, Li X, Yu F, Cai Q, Zhang Z, Wang J, Zhang J, Zhou R, Cheng M, Gao H. J Cardiovasc Pharmacol. 2015 Jan;65(1):13-21. Doi: 10.1097/Fjc.0000000000000157. Critical Role Of Prohibitin In Endothelial Cell Apoptosis Caused By Glycated Low-Den...
Zhou Y, Tan X, Kuang W, Liu L, Wan L. Transl Res. 2012 Jun;159(6):464-72. Doi: 10.1016/J.Trsl.2011.09.007. Epub 2011 Oct 24. Erythromycin Ameliorates Cigarette-Smoke-Induced Emphysema And Inflammation In Rats.
Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X. Exp Ther Med. 2015 May;9(5):1851-1856. Epub 2015 Feb 25. Afatinib Inhibits Proliferation And Invasion And Promotes Apoptosis Of The T24 Bladder Cancer Cell Line.
Wang Jm, Xiao Bl, Zheng Jw, Chen Hb, Zou Sq. World J Gastroenterol. 2007 Jun 21;13(23):3171-5. Effect Of Targeted Magnetic Nanoparticles Containing 5-Fu On Expression Of Bcl-2, Bax And Caspase 3 In Nude Mice With Transplanted Human Liver Cancer.
Meng Qt, Xia Zy, Luo T, Cao C, Zhao B, Wu Y, Wu X, Chen X. Saudi Med J. 2012 Feb;33(2):139-45. Ligustrazine Attenuates Acute Lung Injury Induced By Blunt Chest Trauma.
Zhou Y, Li W, Xu Q, Huang Y. J Exp Clin Cancer Res. 2010 Oct 4;29:131. Doi: 10.1186/1756-9966-29-131. Elevated Expression Of Dickkopf-1 Increases The Sensitivity Of Human Glioma Cell Line Shg44 To Bcnu.
Rasul A, Yu B, Khan M, Zhang K, Iqbal F, Ma T, Yang H. Int J Oncol. 2012 Apr;40(4):1153-61. Doi: 10.3892/Ijo.2011.1277. Epub 2011 Nov 30. Magnolol, A Natural Compound, Induces Apoptosis Of Sgc-7901 Human Gastric Adenocarcinoma Cells Vi...
Peng X, Chen K, Chen J, Fang J, Cui H, Zuo Z, Deng J, Chen Z, Geng Y, Lai W. Environ Toxicol. 2015 Mar 4. Doi: 10.1002/Tox.22120. [Epub Ahead Of Print] Aflatoxin B1 Affects Apoptosis And Expression Of Bax, Bcl-2, And Caspase-3 In Thymus And Bursa ...
Liu Y, Zhou Y, Wang Y, Geng D, Liu J. Obes Surg. 2011 Sep;21(9):1424-31. Doi: 10.1007/S11695-011-0388-Z. Roux-En-Y Gastric Bypass-Induced Improvement Of Glucose Tolerance And Insulin Resistance In Type 2 Diabetic Rats Are Mediated By Glucagon-Like...
Yang Y, Li Zh, Liu H, Shi Wd, Zhang J. Chin J Integr Med. 2015 Jun;21(6):423-30. Doi: 10.1007/S11655-014-1752-3. Epub 2014 May 10. Inhibitory Effect Of Tetramethylpyrazine Preconditioning On Overload Training-Induced Myocardial Apoptosis In Rats.
Song Gh, Wang Rl, Chen Zy, Zhang B, Wang Hl, Liu Ml, Gao Jp, Yan Xy. J Physiol Biochem. 2014 Sep;70(3):761-8. Doi: 10.1007/S13105-014-0344-1. Epub 2014 Jul 30. Toxic Effects Of Sodium Fluoride On Cell Proliferation And Apoptosis Of Leydig Cells Fr...
Chen K, Shu G, Peng X, Fang J, Cui H, Chen J, Wang F, Chen Z, Zuo Z, Deng J, Geng Y, Lai W. Food Chem Toxicol. 2013 Sep;59:446-54. Doi: 10.1016/J.Fct.2013.06.032. Epub 2013 Jun 28. Protective Role Of Sodium Selenite On Histopathological Lesions, D...
Mao Z, Song Z, Li G, Lv W, Zhao X, Li B, Feng X, Chen Y. Neural Regen Res. 2013 Jan 15;8(2):133-42. Doi: 10.3969/J.Issn.1673-5374.2013.02.005. 8-Hydroxy-2-(Di-N-Propylamino)Tetralin Intervenes With Neural Cell Apoptosis Following Diffuse Axonal In...
Li X, Wang L, Wang Z. Phys Med. 2015 Jun;31(4):352-9. Doi: 10.1016/J.Ejmp.2015.02.004. Epub 2015 Feb 19. Radioprotective Activity Of Neutral Polysaccharides Isolated From The Fruiting Bodies Of Hohenbuehelia Serotina.
Wei T, Tian W, Yan H, Shao G, Xie G. Cell Mol Neurobiol. 2014 Nov;34(8):1165-73. Doi: 10.1007/S10571-014-0091-4. Epub 2014 Aug 22. Protective Effects Of Phillyrin On H2O 2-Induced Oxidative Stress And Apoptosis In Pc12 Cells.
Ding H, Wang H, Zhao Y, Sun D, Zhai X. Cell Mol Neurobiol. 2015 Jul;35(5):623-32. Doi: 10.1007/S10571-015-0156-Z. Epub 2015 Jan 18. Protective Effects Of Baicalin On A??????????????-Induced Learning And Memory Deficit, Oxidative Stress, And Apoptosis In Rat.
Xu S, Liu J, Zhang Y, Wang C, Wang J, Yang Y, Huo J, Sun W. Neural Regen Res. 2012 Jun 15;7(17):1318-24. Doi: 10.3969/J.Issn.1673-5374.2012.17.006. Apoptosis-Related Protein Expression In Rabbits With Blast Brain Injury Following Early Hyperbaric ...
Khan M, Li T, Ahmad Khan Mk, Rasul A, Nawaz F, Sun M, Zheng Y, Ma T. Biomed Res Int. 2013;2013:719858. Doi: 10.1155/2013/719858. Epub 2012 Dec 27. Alantolactone Induces Apoptosis In Hepg2 Cells Through Gsh Depletion, Inhibition Of Stat3 Activation...
Tang C, Lu Yh, Xie Jh, Wang F, Zou Jn, Yang Js, Xing Yy, Xi T. Anticancer Drugs. 2009 Apr;20(4):249-58. Doi: 10.1097/Cad.0B013E328327D476. Downregulation Of Survivin And Activation Of Caspase-3 Through The Pi3K/Akt Pathway In Ursolic Acid-Induced ...
Meng Fj, Jiao Sm, Yu B. Int J Mol Med. 2012 Aug;30(2):263-70. Doi: 10.3892/Ijmm.2012.987. Epub 2012 May 9. Picroside Ii Protects Cardiomyocytes From Hypoxia/Reoxygenation-Induced Apoptosis By Activating The Pi3K/Akt And Creb Pathways.
Tian W, Wei T, Li B, Wang Z, Zhang N, Xie G. Plos One. 2014 May 6;9(5):E96775. Doi: 10.1371/Journal.Pone.0096775. Ecollection 2014. Pathway Of Programmed Cell Death And Oxidative Stress Induced By ??-Hydroxybutyrate In Dairy Cow Abomasum Smooth Mus...
Zhang S, Zhang C, Li Y, Wang P, Yue Z, Xie S. Biomed Pharmacother. 2011 Sep;65(6):436-42. Doi: 10.1016/J.Biopha.2011.04.010. Epub 2011 Jun 12. Mir-98 Regulates Cisplatin-Induced A549 Cell Death By Inhibiting Tp53 Pathway.
Xie Sy, Li Yj, Wang Py, Jiao F, Zhang S, Zhang Wj. Oncol Rep. 2010 Jun;23(6):1693-700. Mirna-Regulated Expression Of Oncogenes And Tumor Suppressor Genes In The Cisplatin-Inhibited Growth Of K562 Cells.
Zhang D, Fu M, Song C, Wang C, Lin X, Liu Y. Neural Regen Res. 2012 Oct 25;7(30):2347-53. Doi: 10.3969/J.Issn.1673-5374.2012.30.004. Expressions Of Apoptosis-Related Proteins In Rats With Focal Cerebral Ischemia After Angong Niuhuang Sticker Point...
Yang S, Wang H, Guo Y, Chen S, Zhang My, Shen J, Yu H, Miao J, Wang Hy, Wei W. Int J Biol Sci. 2013 Jul 5;9(6):637-48. Doi: 10.7150/Ijbs.6439. Print 2013. Rmp Plays Distinct Roles In The Proliferation Of Hepatocellular Carcinoma Cells And Normal H...
Li K, Zhu Zc, Liu Yj, Liu Jw, Wang Ht, Xiong Zq, Shen X, Hu Zl, Zheng J. Int J Clin Exp Pathol. 2013 Sep 15;6(11):2460-7. Ecollection 2013. Zfx Knockdown Inhibits Growth And Migration Of Non-Small Cell Lung Carcinoma Cell Line H1299.
Zhou R, Lu Z, Liu K, Guo J, Liu J, Zhou Y, Yang J, Mi M, Xu H. Curr Cancer Drug Targets. 2015;14(9):860-71. Platycodin D Induces Tumor Growth Arrest By Activating Foxo3A Expression In Prostate Cancer In Vitro And In Vivo.
Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.
Li B, Chu X, Gao M, Li W. Acta Biochim Biophys Sin (Shanghai). 2010 Jan;42(1):80-9. Apoptotic Mechanism Of Mcf-7 Breast Cells In Vivo And In Vitro Induced By Photodynamic Therapy With C-Phycocyanin.
Wang Tb, Wei Xq, Lin Wh, Shi Hp, Dong Wg. Clin Exp Med. 2012 Jun;12(2):89-95. Doi: 10.1007/S10238-011-0143-8. Epub 2011 Jun 10. The Inhibition Of Endostar On The Angiogenesis And Growth Of Gastrointestinal Stromal Tumor Xenograft.
Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induced...
Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H. Int J Mol Med. 2014 May;33(5):1298-304. Doi: 10.3892/Ijmm.2014.1684. Epub 2014 Mar 6. Colistin-Induced Apoptosis In Pc12 Cells: Involvement Of The Mitochondrial Apoptotic And Death Receptor Pathways.
Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.
Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ...

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to bcl 2 antibody, bcl-2 antibody, bcl2 antibody