Anti-human CD11b Itgam Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated

ITGAM antibody

Boster Bio Anti-human CD11b Itgam Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Catalog# FC00144-PerCP-Cy5.5). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Product Info Summary

SKU: FC00144-PerCP-Cy5.5
Size: 100 tests, 200 tests
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

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Product Name

Anti-human CD11b Itgam Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated

View all ITGAM Antibodies

SKU/Catalog Number

FC00144-PerCP-Cy5.5

Size

100 tests, 200 tests

Form

Liquid

Description

Boster Bio Anti-human CD11b Itgam Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Catalog# FC00144-PerCP-Cy5.5). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-human CD11b Itgam Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00144-PerCP-Cy5.5)

Host

Mouse

Contents

PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: OKM1

Isotype

IgG2b,k

Immunogen

Human PBMC

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

FC00144-PerCP-Cy5.5 is reactive to ITGAM in Human

Applications

FC00144-PerCP-Cy5.5 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

127.179kDa

Background of ITGAM

ITGAM (Integrin Alpha-M), also called CD11B or Mo1 ALPHA SUBUNIT (MO1A), is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (alphaMbeta2) molecule. A major surface antigen family on human leukocytes includes complement receptor type 3 (CR3A; also called ITGAM, Mac1, or Mo1), lymphocyte function-associated (LFA) antigen type 1 (ITGAL), and p150, 95 (ITGAX). By in situ hybridization, Corbi et al. (1988) demonstrated that the genes encoding the alpha subunits of LFA1 (ITGAL), Mac1, and p150, 95 (ITGAX), all of which are involved in leukocyte adhesion, constitute a cluster on 16p13.1-p11. Callen et al. (1991) narrowed the assignment to 16p11.2. Inflammation plays an essential role in the initiation and progression of atherosclerosis. Simon et al. (2000) presented evidence that it also has a role in vascular repair after mechanical arterial injury (i.e., percutaneous transluminal coronary angioplasty, or PTCA).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

Gene/Protein Information For ITGAM (Source: Uniprot.org, NCBI)

Gene Name

ITGAM

Full Name

Integrin alpha-M

Weight

127.179kDa

Superfamily

integrin alpha chain family

Alternative Names

antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC 1; MAC1; MAC-1; Mac1, alpha subunit; MAC1A; Mac-1a; macrophage antigen alpha polypeptide; Mo1, alpha subunit; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6 ITGAM CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6 integrin subunit alpha M integrin alpha-M|CD11 antigen-like family member B|CR-3 alpha chain|antigen CD11b (p170)|cell surface glycoprotein MAC-1 subunit alpha|complement component 3 receptor 3 subunit|integrin, alpha M (complement component 3 receptor 3 subunit)|leukocyte adhesion receptor MO1|macrophage antigen alpha polypeptide|neutrophil adherence receptor alpha-M subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ITGAM, check out the ITGAM Infographic

ITGAM infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ITGAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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11 Customer Q&As for Anti-human CD11b Itgam Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated

Question

I was wanting to use to test anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00144-PerCP-Cy5.5 on human bone marrow cell for research purposes, then I may be interested in using anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00144-PerCP-Cy5.5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-04-17

Answer

The products we sell, including anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00144-PerCP-Cy5.5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-04-17

Question

My question regarding product FC00144-PerCP-Cy5.5, anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC00144-PerCP-Cy5.5 anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-18

Question

Would FC00144-PerCP-Cy5.5 anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-11-05

Answer

You can see on the product datasheet, FC00144-PerCP-Cy5.5 anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-11-05

Question

I was wanting to use your anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated for Flow Cytometry for human bone marrow cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bone marrow cell identification?

Verified Customer

Verified customer

Asked: 2019-09-10

Answer

It shows on the product datasheet, FC00144-PerCP-Cy5.5 anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-10

Question

I see that the anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00144-PerCP-Cy5.5 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-05-24

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow cell using anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00144-PerCP-Cy5.5. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-21

Question

Do you have a BSA free version of anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00144-PerCP-Cy5.5 available?

E. Jones

Verified customer

Asked: 2018-04-09

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00144-PerCP-Cy5.5 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-04-09

Question

Will anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00144-PerCP-Cy5.5 work for Flow Cytometry with bone marrow cell?

Verified Customer

Verified customer

Asked: 2018-02-26

Answer

According to the expression profile of bone marrow cell, ITGAM is highly expressed in bone marrow cell. So, it is likely that anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00144-PerCP-Cy5.5 will work for Flow Cytometry with bone marrow cell.

Boster Scientific Support

Answered: 2018-02-26

Question

I have attached the WB image, lot number and protocol we used for bone marrow cell using anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC00144-PerCP-Cy5.5. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-08-07

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-08-07

Question

Is this FC00144-PerCP-Cy5.5 anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated reactive to the isotypes of ITGAM?

Verified Customer

Verified customer

Asked: 2017-06-20

Answer

The immunogen of FC00144-PerCP-Cy5.5 anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated is Human lymph node lymphocytes . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-06-20

Question

Is a blocking peptide available for product anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated (FC00144-PerCP-Cy5.5)?

R. Rodriguez

Verified customer

Asked: 2015-05-12

Answer

We do provide the blocking peptide for product anti-human CD11b Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated (FC00144-PerCP-Cy5.5). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-05-12

Order DetailsPrice
FC00144-PerCP-Cy5.5-100tests

100 tests

$360
FC00144-PerCP-Cy5.5-200tests

200 tests

$612

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FC00144-PerCP-Cy5.5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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