Anti-human CD33/Siglec 3 Monoclonal Antibody Unconjugated, Flow Validated

Siglec-3/CD33 antibody

Boster Bio Anti-human CD33/Siglec 3 Monoclonal Antibody Unconjugated, Flow Validated (Catalog# FC01508). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Product Info Summary

SKU: FC01508
Size: 100ug, 500ug
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

Product Name

Anti-human CD33/Siglec 3 Monoclonal Antibody Unconjugated, Flow Validated

View all Siglec-3/CD33 Antibodies

SKU/Catalog Number

FC01508

Size

100ug, 500ug

Form

Liquid

Description

Boster Bio Anti-human CD33/Siglec 3 Monoclonal Antibody Unconjugated, Flow Validated (Catalog# FC01508). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-human CD33/Siglec 3 Monoclonal Antibody Unconjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01508)

Host

Mouse

Contents

PBS pH 7.2, 0.1% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: WM53

Isotype

IgG1,k

Immunogen

Human AML cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC01508 is reactive to CD33 in Human

Applications

FC01508 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

39.825kDa

Background of Siglec-3/CD33

CD33, also known as Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67), is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. CD33 binds sialic acids, therefore is a member of the SIGLEC family of lectins. By fluorescence in situ hybridization, CD33 is mapped to 19q13.3-q13.4.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

Gene/Protein Information For CD33 (Source: Uniprot.org, NCBI)

Gene Name

CD33

Full Name

Myeloid cell surface antigen CD33

Weight

39.825kDa

Superfamily

immunoglobulin superfamily

Alternative Names

CD33 antigen (gp67); CD33 antigen; CD33 molecule; CD33; FLJ00391; gp67; myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec3; Siglec-3; SIGLEC3gp67 CD33 SIGLEC-3, SIGLEC3, p67 CD33 molecule myeloid cell surface antigen CD33|CD33 antigen (gp67)|CD33 molecule transcript|gp67|sialic acid-binding Ig-like lectin 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD33, check out the CD33 Infographic

CD33 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD33: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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2 Customer Q&As for Anti-human CD33/Siglec 3 Monoclonal Antibody Unconjugated, Flow Validated

Question

I am interested in to test anti-human CD33/Siglec 3 Monoclonal antibody Unconjugated, Flow Validated FC01508 on human leukocyte for research purposes, then I may be interested in using anti-human CD33/Siglec 3 Monoclonal antibody Unconjugated, Flow Validated FC01508 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

The products we sell, including anti-human CD33/Siglec 3 Monoclonal antibody Unconjugated, Flow Validated FC01508, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-27

Question

I was wanting to use your anti-human CD33/Siglec 3 Monoclonal antibody Unconjugated, Flow Validated for Flow Cytometry for human leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukocyte identification?

E. Patel

Verified customer

Asked: 2018-07-25

Answer

You can see on the product datasheet, FC01508 anti-human CD33/Siglec 3 Monoclonal antibody Unconjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-07-25

Order DetailsPrice
FC01508-100ug

100ug

$152
FC01508-500ug

500ug

$492

Free Secondary Antibody

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FC01508
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$152.00

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