Anti-human CD33/Siglec 3 Monoclonal Antibody Unconjugated, Flow Validated

Product Name

Anti-human CD33/Siglec 3 Monoclonal Antibody Unconjugated, Flow Validated

View all Siglec-3/CD33 Antibodies

SKU/Catalog Number

FC01508

Size

100ug, 500ug

Form

Liquid

Description

Boster Bio Anti-human CD33/Siglec 3 Monoclonal Antibody Unconjugated, Flow Validated (Catalog# FC01508). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-human CD33/Siglec 3 Monoclonal Antibody Unconjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01508)

Host

Mouse

Contents

PBS pH 7.2, 0.1% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: WM53

Isotype

IgG1,k

Immunogen

Human AML cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC01508 is reactive to CD33 in Human

Applications

FC01508 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

39.825kDa

Background of Siglec-3/CD33

CD33, also known as Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67), is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. CD33 binds sialic acids, therefore is a member of the SIGLEC family of lectins. By fluorescence in situ hybridization, CD33 is mapped to 19q13.3-q13.4.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

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Monocytes gated human PBMCs stained with mouse anti-human CD33 (clone WM53) followed by staining with goat anti-mouse IgG(H+L) Alexa Fluor 488 (red histogram). Blue histogram is for the monocytes gated PBMCs stained with purified mouse IgG1 (MOPC31C) isotype control followed by staining with goat anti-mouse IgG(H+L) Alexa Fluor 488.

Gene/Protein Information For CD33 (Source: Uniprot.org, NCBI)

Alternative Names

CD33 antigen (gp67); CD33 antigen; CD33 molecule; CD33; FLJ00391; gp67; myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec3; Siglec-3; SIGLEC3gp67 CD33 SIGLEC-3, SIGLEC3, p67 CD33 molecule myeloid cell surface antigen CD33|CD33 antigen (gp67)|CD33 molecule transcript|gp67|sialic acid-binding Ig-like lectin 3

For more info on CD33, check out the CD33 Infographic

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In this infographic, you will see the following information for CD33: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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