Anti-Human CD4 Monoclonal Antibody mFluor450 Conjugated, Flow Validated

Product Name

Anti-Human CD4 Monoclonal Antibody mFluor450 Conjugated, Flow Validated

View all CD4 Antibodies

SKU/Catalog Number

FC00344-mFluor450

Size

100 tests, 200 tests

Form

Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA

Description

Boster Bio Anti-Human CD4 Monoclonal Antibody mFluor450 Conjugated, Flow Validated (Catalog# FC00344-mFluor450). Tested in Flow Cytometry application(s). This antibody reacts with Human, Rhesus monkey, Cynomolgus monkey.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Human CD4 Monoclonal Antibody mFluor450 Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00344-mFluor450)

Host

Mouse

Contents

PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: OKT4

Isotype

IgG2b,k

Immunogen

Human peripheral blood T lymphocytes

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC00344-mFluor450 is reactive to CD4 in Cynomolgus monkey, Human, Rhesus monkey

Applications

FC00344-mFluor450 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

51.111kDa

Background of CD4

B-lymphocyte antigen CD19, also known as CD19 (Cluster of Differentiation 19), is a protein that in humans is encoded by the CD19 gene. It is found on the surface of B-cells, a type of white blood cell. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. The CD19 gene encodes a cell surface molecule that assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

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Lymphocytes gated blood (RBC lysed) stained with mFluor 450 conjugated anti-human CD4 (clone OKT-4, red histogram). Lymphocytes gated blood (RBC lysed) stained with FL-6 FMO control (blue histogram). The data were generated in Beckman Coulter CytoFLEX S Flow Cytometer and analyzed by using FlowJo software.

Gene/Protein Information For CD4 (Source: Uniprot.org, NCBI)

Alternative Names

CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 CD4 CD4mut CD4 molecule T-cell surface glycoprotein CD4|CD4 antigen (p55)|CD4 receptor|T-cell surface antigen T4/Leu-3

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In this infographic, you will see the following information for CD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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