Anti-Human CD46 DyLight® 488 conjugated Antibody(monoclonal, 9E9)

CD46 antibody

Boster Bio Anti-Human CD46 DyLight® 488 conjugated Antibody (monoclonal, 9E9) catalog # M00377-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M00377-Dyl488
Size: 100 μg/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

Product Name

Anti-Human CD46 DyLight® 488 conjugated Antibody(monoclonal, 9E9)

View all CD46 Antibodies

SKU/Catalog Number

M00377-Dyl488

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human CD46 DyLight® 488 conjugated Antibody (monoclonal, 9E9) catalog # M00377-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human CD46 DyLight® 488 conjugated Antibody(monoclonal, 9E9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00377-Dyl488)

Host

Mouse

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN5.

Clonality

Monoclonal

Clone Number

9E9

Isotype

Mouse IgG1

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CD46.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M00377-Dyl488 is reactive to CD46 in Human

Applications

M00377-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

43.747kDa

Background of CD46

CD46 complement regulatory protein also known as CD46 (cluster of differentiation 46) and Membrane Cofactor Protein is a protein which in humans is encoded by the CD46 gene. The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. And the encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x106 cells, human

Validation Images & Assay Conditions

Gene/Protein Information For CD46 (Source: Uniprot.org, NCBI)

Gene Name

CD46

Full Name

Membrane cofactor protein

Weight

43.747kDa

Alternative Names

AHUS2; CD46 antigen; CD46 molecule, complement regulatory protein; CD46; MCP; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); MIC10; TLXcomplement regulatory protein; trophoblast leucocyte common antigen; Trophoblast leukocyte common antigen; trophoblast-lymphocyte cross-reactive antigen CD46 AHUS2, MCP, MIC10, TLX, TRA2.10 CD46 molecule membrane cofactor protein|CD46 antigen, complement regulatory protein|CD46 molecule, complement regulatory protein|antigen identified by monoclonal antibody TRA-2-10|complement membrane cofactor protein|measles virus receptor|membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen)|trophoblast leucocyte common antigen|trophoblast leukocyte common antigen|trophoblast-lymphocyte cross-reactive antigen

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD46, check out the CD46 Infographic

CD46 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD46: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Human CD46 DyLight® 488 conjugated Antibody(monoclonal, 9E9)

Question

We are currently using anti-Human CD46 DyLight® 488 conjugated antibody(monoclonal, 9E9) M00377-Dyl488 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2017-09-27

Answer

The anti-Human CD46 DyLight® 488 conjugated antibody(monoclonal, 9E9) (M00377-Dyl488) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-09-27

Question

We have been able to see staining in human sperm. Any tips? Is anti-Human CD46 DyLight® 488 conjugated antibody(monoclonal, 9E9) supposed to stain sperm positively?

Verified Customer

Verified customer

Asked: 2017-06-07

Answer

According to literature sperm does express CD46. According to Uniprot.org, CD46 is expressed in palpebral conjunctiva, testis, teratocarcinoma, fetal kidney, liver salivary gland, liver, sperm, among other tissues. Regarding which tissues have CD46 expression, here are a few articles citing expression in various tissues:
Fetal kidney, Liver, and Salivary gland, Pubmed ID: 17974005
Liver, Pubmed ID: 16710414, 24275569
Sperm, Pubmed ID: 1479546
Teratocarcinoma, Pubmed ID: 14702039
Testis, Pubmed ID: 8418811, 9659228, 15489334

Boster Scientific Support

Answered: 2017-06-07

Question

My team were content with the WB result of your anti-Human CD46 DyLight® 488 conjugated antibody(monoclonal, 9E9). However we have seen positive staining in teratocarcinoma secretory vesicle, using this antibody. Is that expected? Could you tell me where is CD46 supposed to be expressed?

T. Singh

Verified customer

Asked: 2013-03-04

Answer

Based on literature, teratocarcinoma does express CD46. Generally CD46 expresses in cytoplasmic vesicle, secretory vesicle,. Regarding which tissues have CD46 expression, here are a few articles citing expression in various tissues:
Fetal kidney, Liver, and Salivary gland, Pubmed ID: 17974005
Liver, Pubmed ID: 16710414, 24275569
Sperm, Pubmed ID: 1479546
Teratocarcinoma, Pubmed ID: 14702039
Testis, Pubmed ID: 8418811, 9659228, 15489334

Boster Scientific Support

Answered: 2013-03-04

Order DetailsPrice
M00377-Dyl488

100μg

$515
M00377-Dyl488-carrier-free

Carrier Free

$515

Free Secondary Antibody

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M00377-Dyl488
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$515.00

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