Product Info Summary
SKU: | A00480-Dyl488 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Human CD5 DyLight® 488 conjugated Antibody
SKU/Catalog Number
A00480-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human CD5 DyLight® 488 conjugated Antibody catalog # A00480-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human CD5 DyLight® 488 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00480-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CD5, which shares 92.1% and 89.5% amino acid (aa) sequence identity with mouse and rat CD5, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00480-Dyl488 is reactive to CD5 in Human
Applications
A00480-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
54.578kDa
Background of CD5
CD5 is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. In humans, the gene is located on the long arm of chromosome 11. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For CD5 (Source: Uniprot.org, NCBI)
Gene Name
CD5
Full Name
T-cell surface glycoprotein CD5
Weight
54.578kDa
Alternative Names
CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1 CD5 LEU1, T1 CD5 molecule T-cell surface glycoprotein CD5|CD5 antigen (p56-62)|epididymis secretory sperm binding protein|lymphocyte antigen T1/Leu-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD5, check out the CD5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD5 DyLight® 488 conjugated Antibody (A00480-Dyl488)
Hello CJ!
No publications found for A00480-Dyl488
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Human CD5 DyLight® 488 conjugated Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Human CD5 DyLight® 488 conjugated Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-Human CD5 DyLight® 488 conjugated Antibody
Question
My boss were well pleased with the WB result of your anti-Human CD5 DyLight® 488 conjugated antibody. However we have seen positive staining in lymphocyte cell membrane using this antibody. Is that expected? Could you tell me where is CD5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
From what I have seen in literature, lymphocyte does express CD5. Generally CD5 expresses in cell membrane. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-09-19
Question
We are currently using anti-Human CD5 DyLight® 488 conjugated antibody A00480-Dyl488 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
E. Bhatt
Verified customer
Asked: 2018-12-07
Answer
The anti-Human CD5 DyLight® 488 conjugated antibody (A00480-Dyl488) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-07
Question
We have been able to see staining in human leukocyte. Do you have any suggestions? Is anti-Human CD5 DyLight® 488 conjugated antibody supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
From what I have seen in literature leukocyte does express CD5. From what I have seen in Uniprot.org, CD5 is expressed in leukocyte, lymphocyte, thymus, pancreas, leukemic t-cell, among other tissues. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-07-19