Anti-Fibroblast growth factor 1 FGF1 Antibody
Rabbit IgG polyclonal antibody for Fibroblast growth factor 1(FGF1) detection. Tested with WB, IHC-P, ELISA in Human. Cited in 2 publication(s).
Anti-Fibroblast growth factor 1 FGF1 Antibody Info At A Glance
|Applications:||ELISA, IHC, WB|
|Product Name||Anti-Fibroblast growth factor 1 FGF1 Antibody
See all FGF acidic/FGF1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for FGF acidic/FGF1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. FGF acidic/FGF1 information: Molecular Weight: 17460 MW; Subcellular Localization: Secreted. Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytosol. Nucleus. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1 (By similarity). Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59; Tissue Specificity: Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle.|
|Cite This Product||Anti-Fibroblast growth factor 1 FGF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1005)|
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|Anti-Fibroblast growth factor 1 FGF1 Antibody may replace the following items: sc 13123|sc 1359|sc 1884|sc 4572|sc 55520|sc 7910.|
|Applications||ELISA, IHC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||E. coli-derived human FGF1 recombinant protein(Position: F16-D155).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For FGF1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||2246|
|Species Of This Entry||Human|
|Protein Name||Fibroblast growth factor 1|
|Superfamily||heparin-binding growth factors family|
|Alternative Names||FGF acidic/FGF1|AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Glycosylation; Sulphation; Biotinylation; Reduction; Acetylation; Oxidation; Dephosphorylation|
|Subcellular Localization||Secreted. Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytosol. Nucleus. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1 (By similarity). Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59.|
|Gene Location||5q31.3, on Chromasome 5, gene sequence: NC_000005.10 (142592178..142698070, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For FGF1 (Source: Uniprot.org, NCBI)
|Protein Function||Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:18441324, PubMed:20422052). Can induce angiogenesis (PubMed:23469107).|
|Tissue Specificity||Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle.|
|Interacting Proteins||FGFR1; FGFR2; FGFR3|
|Related Diseases||Neoplasms; Malignant Neoplasms; Tumor Angiogenesis; Pathologic Neovascularization; Carcinoma; Mammary Neoplasms; Malignant Paraganglionic Neoplasm; Malignant Neoplasm Of Breast; Cell Transformation, Neoplastic; Nervousness; Inflammation; Tumor Progression; Tissue Adhesions; Neoplasm Metastasis; Hypoxia; Malignant Neoplasm Of Prostate; Leukemia; Hyperplasia; Ischemia|
|Related Pathways||Cell Proliferation; Angiogenesis; Secretion; Cell Growth; Localization; Cell Cycle; Cell Differentiation; Cell Death; Regeneration; Neurogenesis; Wound Healing; Sulfation; Translation; Neuroprotection; Cell Migration; Lung Development; Epithelial Cell Proliferation; Lung Morphogenesis; Regulation Of Growth; Liver Development|
|Background||Fibroblast growth factor 1(acidic), also known as FGF1/ECGF/HBGF1, is a human gene which is mapped to 5q31. Human FGF1 shares 96% amino acid sequence homology with both rat and mouse. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is though to be involved in organogenesis. Additionally, Acidic fibroblast growth factor is derived from beta-endothelial cell growth factor(ECGFB) by posttranslational processing. Alpha-ECGF is also derived from ECGFB in the same manner.|
|Scientific References||PMID: 3523756 by Jaye M., et al. Human endothelial cell growth factor: cloning, nucleotide sequence, and chromosome localization.|
PMID: 2590193 by Mergia A., et al. Structural analysis of the gene for human acidic fibroblast growth factor.
Our Boster Quality Guarantee for Anti-Fibroblast growth factor 1 FGF1 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
IHC(P): Human Mammary Cancer Tissue
Lane 1: Recombinant Human FGF1 Protein 10ng
Lane 2: Recombinant Human FGF1 Protein 5ng
Lane 3: Recombinant Human FGF1 Protein 2
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 2
|Zhou Y, Ren L, Zhu J, Yan S, Wang H, Song N, Li L, Ouyang H, Pang D. Mol Cell Biochem. 2011 Aug;354(1-2):39-46. Doi: 10.1007/S11010-011-0803-8. Epub 2011 Apr 3. Construction Of A Recombinant Human Fgf1 Expression Vector For Mammary Gland-Specific ...||PubMed ID 21461910|
|Zhang L, Wang H, Wang T, Jiang N, Yu P, Chong Y, Fu F. Exp Ther Med. 2015 Mar;9(3):972-976. Epub 2014 Dec 24. Ferulic Acid Ameliorates Nerve Injury Induced By Cerebral Ischemia In Rats.||PubMed ID 25667662|
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Note: sample size takes longer to process, average 5-7 days.
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