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Product Info Summary
| SKU: | RP1005 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Fibroblast growth factor 1 FGF1 Antibody
View all FGF acidic/FGF1 Antibodies
SKU/Catalog Number
RP1005
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fibroblast growth factor 1 FGF1 Antibody catalog # RP1005. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fibroblast growth factor 1 FGF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1005)
Host
Rabbit
Contents
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human FGF1 recombinant protein (Position: F16-D155).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1005 is reactive to FGF1 in Human
Applications
RP1005 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
26 kDa
Calculated molecular weight
17.46kDa
Background of FGF acidic/FGF1
Fibroblast growth factor 1 (acidic), also known as FGF1/ECGF/HBGF1, is a human gene which is mapped to 5q31. Human FGF1 shares 96% amino acid sequence homology with both rat and mouse. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is though to be involved in organogenesis. Additionally, Acidic fibroblast growth factor is derived from beta-endothelial cell growth factor (ECGFB) by posttranslational processing. Alpha-ECGF is also derived from ECGFB in the same manner.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-human FGF1 antibody, RP1005, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Anti-human FGF1 antibody, RP1005, Western blotting
Lane 1: Recombinant Human FGF1 Protein 10ng
Lane 2: Recombinant Human FGF1 Protein 5ng
Lane 3: Recombinant Human FGF1 Protein 2
Protein Target Info & Infographic
Gene/Protein Information For FGF1 (Source: Uniprot.org, NCBI)
Gene Name
FGF1
Full Name
Fibroblast growth factor 1
Weight
17.46kDa
Superfamily
heparin-binding growth factors family
Alternative Names
AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1 FGF1 AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-1, FGF-alpha, FGFA, GLIO703, HBGF-1, HBGF1 fibroblast growth factor 1 fibroblast growth factor 1|beta-endothelial cell growth factor|endothelial cell growth factor, alpha|endothelial cell growth factor, beta|fibroblast growth factor 1 (acidic)|heparin-binding growth factor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGF1, check out the FGF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fibroblast growth factor 1 FGF1 Antibody (RP1005)
Hello CJ!
RP1005 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang L, Wang H, Wang T, Jiang N, Yu P, Chong Y, Fu F. Exp Ther Med. 2015 Mar;9(3):972-976. Epub 2014 Dec 24. Ferulic Acid Ameliorates Nerve Injury Induced By Cerebral Ischemia In Rats.
Zhou Y, Ren L, Zhu J, Yan S, Wang H, Song N, Li L, Ouyang H, Pang D. Mol Cell Biochem. 2011 Aug;354(1-2):39-46. Doi: 10.1007/S11010-011-0803-8. Epub 2011 Apr 3. Construction Of A Recombinant Human Fgf1 Expression Vector For Mammary Gland-Specific ...
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Customer Q&As
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3 Customer Q&As for Anti-Fibroblast growth factor 1 FGF1 Antibody
Question
I have a question about product RP1005, anti-FGF1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1005 anti-FGF1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-08
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain stem using anti-FGF1 antibody RP1005. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-05
Question
We are currently using anti-FGF1 antibody RP1005 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
E. Miller
Verified customer
Asked: 2016-05-12
Answer
The anti-FGF1 antibody (RP1005) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-05-12
