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Product Info Summary
| SKU: | M11271 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, ICC |
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Product info
Product Name
Anti-Human IgA2 IGHA2 Rabbit Monoclonal Antibody, Clone#RM125
SKU/Catalog Number
M11271
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgA2 IGHA2 Rabbit Monoclonal Antibody, Clone#RM125 (Catalog # M11271). Tested in ELISA, ICC, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgA2 IGHA2 Rabbit Monoclonal Antibody, Clone#RM125 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11271)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM125
Isotype
Rabbit IgG
Immunogen
IgA2
Cross-reactivity
This antibody reacts to human IgA2. No cross-reactivity with human IgA1, IgG, IgM, IgD, or IgE.
Reactive Species
M11271 is reactive to IGHA2 in Human
Calculated molecular weight
36.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11271 is guaranteed for ELISA, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 50ng/well – 200ng/well (for Capture)
0.05ug/mL – 0.2ug/mL (for Detection)
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL
Immunohistochemistry (IHC): 0.1ug/mL-1ug/mL.
Validation Images & Assay Conditions
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IHC result
Immunohistochemistry of human tonsil tissue using anti-human IgA2 antibody RM125
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ELISA result showing specificity
Sandwich ELISA using RM125 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin
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ELISA result showing specificity
Sandwich ELISA using RM125 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin
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ELISA result showing specificity
ELISA of human immunoglobulins shows RM125 only reacted to human IgA2. No cross reactivity with human IgA1, IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM125 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
A titer ELISA using RM125. The plate was coated with different amounts of human IgA2. A serial dilution of RM125 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Specific Publications For Anti-Human IgA2 IGHA2 Rabbit Monoclonal Antibody, Clone#RM125 (M11271)
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Customer Q&As
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5 Customer Q&As for Anti-Human IgA2 IGHA2 Rabbit Monoclonal Antibody, Clone#RM125
Question
I was wanting to use your anti-Human IgA2 Rabbit Monoclonal antibody, Clone#RM125 for ELISA for human saliva on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human saliva identification?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
It shows on the product datasheet, M11271 anti-Human IgA2 Rabbit Monoclonal antibody, Clone#RM125 has been validated for ELISA, IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human saliva in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-02
Question
Does anti-Human IgA2 Rabbit Monoclonal antibody, Clone#RM125 M11271 work for ELISA with saliva?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
According to the expression profile of saliva, IGHA2 is highly expressed in saliva. So, it is likely that anti-Human IgA2 Rabbit Monoclonal antibody, Clone#RM125 M11271 will work for ELISA with saliva.
Boster Scientific Support
Answered: 2019-08-20
Question
Is this M11271 anti-Human IgA2 Rabbit Monoclonal antibody, Clone#RM125 reactive to the isotypes of IGHA2?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
The immunogen of M11271 anti-Human IgA2 Rabbit Monoclonal antibody, Clone#RM125 is IgA2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-29
Question
We are currently using anti-Human IgA2 Rabbit Monoclonal antibody, Clone#RM125 M11271 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
J. Li
Verified customer
Asked: 2019-02-18
Answer
The anti-Human IgA2 Rabbit Monoclonal antibody, Clone#RM125 (M11271) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-18
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for saliva using anti-Human IgA2 Rabbit Monoclonal antibody, Clone#RM125 M11271. Let me know if you need anything else.
S. Wu
Verified customer
Asked: 2018-03-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-16
