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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||IF, IHC, ICC, WB|
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Anti-Human Ki67 Antibody
Boster Bio Anti-Human Ki67 Antibody catalog # M00254-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human Ki67 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00254-3)
Serum with 5mM NaN3
Recombinant human construct containing amino acids 1,111-1,490 expressed in and purified from E. coli.
M00254-3 is reactive to MKI67 in Human, Mouse, Rat
M00254-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WB: 1:1,000-5,000. IF 1:2,000-5,000, IHC 1:1,000
Validation Images & Assay Conditions
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Western blot analysis of equal amounts of cell lysates using rabbit pAb to Ki-67 M00254-3, dilution 1:10,000, (green):  protein standard (red),  HeLa cells,  HEK293 cells. Strong double bands above 250kDa correspond to the two major Ki-67 isoforms of molecular weight 345kDa and 395kDa. Smaller proteolytic fragments of these isoforms are also detected on the blot.
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Immunofluorescent analysis of HeLa cells stained with rabbit pAb to Ki-67 M00254-3, dilution 1:5,000 in red, and mouse monoclonal antibody to fibrillarin, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. The Ki-67 protein accumulates
Gene/Protein Information For MKI67 (Source: Uniprot.Org, NCBI)
Proliferation marker protein Ki-67
antigen identified by monoclonal Ki-67; Antigen Ki67; antigen KI-67; antiKi67; Ki67 ihc; Ki-67 ihc; Ki67 mouse; Ki-67 mouse; Ki67 western blot; Ki-67 western blot; Ki67; Ki-67; KIA; Marker Of Proliferation Ki-67; MIB-; MIB-1; MKI67; PPP1R105; Proliferation Marker Protein Ki-67; proliferation-related Ki-67 antigen; Protein Phosphatase 1; Regulatory Subunit 105; TSG126 MKI67 KIA, MIB-, MIB-1, PPP1R105 marker of proliferation Ki-67 proliferation marker protein Ki-67|antigen Ki67|antigen identified by monoclonal antibody Ki-67|proliferation-related Ki-67 antigen|protein phosphatase 1, regulatory subunit 105*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MKI67, check out the MKI67 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MKI67: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
M00254-3 has been cited in 15 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xiaozhu Tian,Rong Li,Yiming Jiang,Fei Zhao,Zhengsheng Yu,Yiqing Wang,Zixing Dong,Pu Liu,Xiangkai Li,Bifidobacterium breve ATCC15700 pretreatment prevents alcoholic liver disease through modulating gut microbiota in mice exposed to chronic alcohol intake,J
M00254-3 usage in article: APP:IHC, SAMPLE:LIVER TISSUE, DILUTION:NA
Xu X,Li L,Li X,Tao D,Zhang P,Gong J.Aptamer-protamine-siRNA nanoparticles in targeted therapy of ErbB3 positive breast cancer cells.Int J Pharm.2020 Nov 30;590:119963.doi:10.1016/j.ijpharm.2020.119963.Epub 2020 Oct 9.PMID:33039492.
M00254-3 usage in article: APP:WB, SAMPLE:MCF-7 CELLS, DILUTION:NA
Yang Z,Zhang J,Lu D,Sun Y, Zhao X,Wang X,Zhou W,He Q,Jiang Z.Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge. Cancer Cell Int.2020 Mar 4;20:67. doi:10.1186/s1293 5-020-1150-1.PMID:32158357;PMC
M00254-3 usage in article: APP:WB, SAMPLE:SW480 AND HCT116 CELLS, DILUTION:NA
Zhang L, Sun D, Zhang J, Tian Y. Circ-UBR1 facilitates proliferation, metastasis, and inhibits apoptosis in breast cancer by regulating the miR-1299/CCND1 axis. Life Sci. 2020 Nov 28;266:118829. doi: 10.1016/j.lfs.2020.118829. Epub ahead of print. PMID: 3
Species: Human, Mouse
M00254-3 usage in article: APP:IHC, SAMPLE:SUBUTANEOUS TUMOR TISSUE, DILUTION:1:400
Deletion of %u03B2-Arrestin2 in Mice Limited Pancreatic %u03B2-Cell Expansion under Metabolic Stress through Activation of the JNK Pathway
KDM4B and KDM4A promote endometrial cancer progression by regulating androgen receptor, c-myc, and p27kip1
WX-132-18B, a novel microtubule inhibitor, exhibits promising anti-tumor effects
Nek7 is overexpressed in hepatocellular carcinoma and promotes hepatocellular carcinoma cell proliferation in vitro and in vivo
Soy isoflavone extracts stimulate the growth of nude mouse xenografts bearing estrogen-dependent human breast cancer cells (MCF-7)
Pan Z, Tian Y, Zhang B, Zhang X, Shi H, Liang Z, Wu P, Li R, You B, Yang L, Mao F, Qian H, Xu W. Int J Oncol. 2017 Oct;51(4):1055-1066. doi: 10.3892/ijo.2017.4101. Epub 2017 Aug 23. YAP signaling in gastric cancer-derived mesenchymal stem cells is...
Cheng Z, Wang Hz, Li X, Wu Z, Han Y, Li Y, Chen G, Xie X, Huang Y, Du Z, Zhou Y. J Exp Clin Cancer Res. 2015 Mar 26;34:27. Doi: 10.1186/S13046-015-0142-9. Microrna-184 Inhibits Cell Proliferation And Invasion, And Specifically Targets Tnfaip2 In G...
Zhao Xy, Chen L, Li Yh, Xu Q. World J Gastroenterol. 2007 Dec 28;13(48):6558-61. Plexina1 Expression In Gastric Carcinoma And Its Relationship With Tumor Angiogenesis And Proliferation.
Li Xt, Wang Hz, Wu Zw, Yang Tq, Zhao Zh, Chen Gl, Xie Xs, Li B, Wei Yx, Huang Yl, Zhou Yx, Du Zw. Cell Mol Neurobiol. 2015 Jul;35(5):679-87. Doi: 10.1007/S10571-015-0163-0. Epub 2015 Feb 8. Mir-494-3P Regulates Cellular Proliferation, Invasion, Mi...
Yi M, Yang J, Chen X, Li J, Li X, Wang L, Tan Y, Xiong W, Zhou M, Mccarthy Jb, Li G, Xiang B, Xie H. J Cell Physiol. 2011 Sep;226(9):2360-9. Doi: 10.1002/Jcp.22568. Rassf1A Suppresses Melanoma Development By Modulating Apoptosis And Cell-Cycle Pro...
Yuan Y, Zhang J, Cai L, Ding C, Wang X, Chen H, Wang X, Yan J, Lu J. Oncol Rep. 2013 Jun;29(6):2291-6. Doi: 10.3892/Or.2013.2390. Epub 2013 Apr 8. Leptin Induces Cell Proliferation And Reduces Cell Apoptosis By Activating C-Myc In Cervical Cancer.
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