Anti-Iba1 AIF1 Monoclonal Antibody

Boster Bio Anti-Iba1 AIF1 Monoclonal Antibody catalog # M01394. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 8 publication(s).

Product Info Summary

SKU: M01394
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

Product Name

Anti-Iba1 AIF1 Monoclonal Antibody

See all AIF-1/Iba1 products

SKU/Catalog Number

M01394

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Iba1 AIF1 Monoclonal Antibody catalog # M01394. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Iba1 AIF1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01394)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEBB-1

Immunogen

A synthesized peptide derived from human Iba1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01394 is reactive to AIF1 in Human, Mouse, Rat

Applications

M01394 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500~1:1000
IHC 1:100~1:500
ICC/IF 1:50~1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For AIF1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P55008

Gene ID

199

Gene Name

AIF1

Full Name

Allograft inflammatory factor 1

Weight

16.703kDa

Alternative Names

AIF1; AIF-1; AIF-1IRT1; allograft inflammatory factor 1; G1; IBA1; IBA1Em:AF129756.17; IbaI; interferon gamma responsive transcript; Ionized calcium-binding adapter molecule 1; IRT1; IRT-1; Protein G1 AIF1 AIF-1, IBA1, IRT-1, IRT1 allograft inflammatory factor 1 allograft inflammatory factor 1|interferon gamma responsive transcript|ionized calcium-binding adapter molecule 1|protein G1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on AIF1, check out the AIF1 Infographic

AIF1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for AIF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M01394 has been cited in 8 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
M01394 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:1000

Li X,Shi MQ,Chen C,Du JR.Phthalide derivative CD21 ameliorates ischemic brain injury in a mouse model of global cerebral ischemia: involvement of inhibition of NLRP3.Int Immunopharmacol.2020 Sep;86:106714.doi: 10.1016/j.intimp.2020.106714.Epub 2020 Jun 24
Species: Mouse
M01394 usage in article: APP:IF, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:500

Zhou Z,Hou J,Mo Y,Ren M,Yang G,Qu Z,Hu Y.Geniposidic acid ameliorates spatial learning and memory deficits and alleviates neuroinflammation via inhibiting HMGB-1 and downregulating TLR4/2 signaling pathway in APP/PS1 mice.Eur J Pharmacol.2020 Feb 15;869:1
Species: Mouse
M01394 usage in article: APP:IHC, SAMPLE:BRAIN SECTION, DILUTION:1:400

Mohamed MY,Masocha W. Indomethacin augments lipopolysaccharide-induced expression of inflammatory molecules in the mouse brain. PeerJ.2020 Nov 18;8:e10391.doi:10.7717/peerj.10391.PMID:33240677;PMCID:PMC7680052.
Species: Mouse
M01394 usage in article: APP:WB, SAMPLE:BRAIN HOMOGENATES, DILUTION:1:50

Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5a/PKC Signaling Pathway

Mechanism of apoptosis induction in human hepatocellular carcinoma cells following treatment with a gecko peptides mixture

Toll-like receptor 4 contributes to chronic itch, alloknesis, and spinal astrocyte activation in male mice

Re-infection of the prion from the scrapie?infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c

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17 Customer Q&As for Anti-Iba1 AIF1 Monoclonal Antibody

Question

Is there a protocol for M01394 in which it was tested positive?

Verified customer

Asked: 2021-02-18

Answer

We recommend the use of the Anti-Iba1 AIF1 Monoclonal Antibody (M01394) in methanol fixed IHC stainings of mouse brain cryosections. The application was customer reported.

Boster Scientific Support

Answered: 2021-02-22

Question

Here is the WB image, lot number and protocol we used for lymphocyte using anti-Iba1 Monoclonal antibody M01394. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-24

Question

We are currently using anti-Iba1 Monoclonal antibody M01394 for mouse tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

The anti-Iba1 Monoclonal antibody (M01394) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-03

Question

I am interested in to test anti-Iba1 Monoclonal antibody M01394 on human lymphocyte for research purposes, then I may be interested in using anti-Iba1 Monoclonal antibody M01394 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-05

Answer

The products we sell, including anti-Iba1 Monoclonal antibody M01394, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-05

Question

We have tried in the past anti-Iba1 Monoclonal antibody for WB on liver in a previous project. I am using rat, and We are going to use the antibody for IHC next. My question regards examining liver as well as erythroleukemia in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

I looked at the website and datasheets of our anti-Iba1 Monoclonal antibody and I see that M01394 has been tested on rat in both WB and IHC. Thus M01394 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-09-04

Question

My boss were satisfied with the WB result of your anti-Iba1 Monoclonal antibody. However we have been able to see positive staining in erythroleukemia cytoskeleton using this antibody. Is that expected? Could you tell me where is AIF1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-07-31

Answer

From what I have seen in literature, erythroleukemia does express AIF1. Generally AIF1 expresses in cytoplasm, cytoskeleton. Regarding which tissues have AIF1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Glial tumor, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymphocyte, Pubmed ID: 8912632
Prostate, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-07-31

Question

I was wanting to use your anti-Iba1 Monoclonal antibody for IF for human lymphocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymphocyte identification?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

You can see on the product datasheet, M01394 anti-Iba1 Monoclonal antibody has been tested for IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lymphocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-25

Question

Will anti-Iba1 Monoclonal antibody M01394 work for IF with lymphocyte?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

According to the expression profile of lymphocyte, AIF1 is highly expressed in lymphocyte. So, it is likely that anti-Iba1 Monoclonal antibody M01394 will work for IF with lymphocyte.

Boster Scientific Support

Answered: 2019-07-01

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymphocyte using anti-Iba1 Monoclonal antibody M01394. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-28

Question

I see that the anti-Iba1 Monoclonal antibody M01394 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-05-21

Question

We need using your anti-Iba1 Monoclonal antibody for positive regulation of protein phosphorylation studies. Has this antibody been tested with western blotting on rat thymus tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

Thank you for your inquiry. This M01394 anti-Iba1 Monoclonal antibody is tested on mouse thymus tissue, brain tissue, rat thymus tissue, spleen tissue. It is guaranteed to work for IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-05-16

Question

Is this M01394 anti-Iba1 Monoclonal antibody reactive to the isotypes of AIF1?

Verified Customer

Verified customer

Asked: 2018-07-09

Answer

The immunogen of M01394 anti-Iba1 Monoclonal antibody is A synthesized peptide derived from human Iba1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-07-09

Question

We have observed staining in mouse liver. Are there any suggestions? Is anti-Iba1 Monoclonal antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2018-02-19

Answer

From what I have seen in literature liver does express AIF1. From what I have seen in Uniprot.org, AIF1 is expressed in metanephric glomerulus, lymphocyte, glial tumor, prostate, erythroleukemia, liver, among other tissues. Regarding which tissues have AIF1 expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Glial tumor, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymphocyte, Pubmed ID: 8912632
Prostate, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-02-19

Question

Is a blocking peptide available for product anti-Iba1 Monoclonal antibody (M01394)?

Verified Customer

Verified customer

Asked: 2017-11-09

Answer

We do provide the blocking peptide for product anti-Iba1 Monoclonal antibody (M01394). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-11-09

Question

Is there a BSA free version of anti-Iba1 Monoclonal antibody M01394 available?

Verified Customer

Verified customer

Asked: 2017-08-24

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Iba1 Monoclonal antibody M01394 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-08-24

Question

Can you help my question with product M01394, anti-Iba1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

D. Jha

Verified customer

Asked: 2017-03-06

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01394 anti-Iba1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-03-06

Question

Will M01394 anti-Iba1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

D. Carter

Verified customer

Asked: 2013-12-03

Answer

It shows on the product datasheet, M01394 anti-Iba1 Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-12-03

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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M01394
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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