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Product Info Summary
| SKU: | A03671-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-ICAD (Q177) DFFA Antibody
View all DFF45/ICAD Antibodies
SKU/Catalog Number
A03671-3
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ICAD (Q177) DFFA Antibody catalog # A03671-3. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ICAD (Q177) DFFA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03671-3)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human KCNQ4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03671-3 is reactive to DFFA in Human, Mouse, Rat
Applications
A03671-3 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
36.522kDa
Background of DFF45/ICAD
Probably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. KCNQ4 channels are blocked by linopirdin, XE991 and bepridil, whereas clofilium is without significant effect. Muscarinic agonist oxotremorine-M strongly suppress KCNQ4 current in CHO cells in which cloned KCNQ4 channels were coexpressed with M1 muscarinic receptors.
Kubisch C., Cell 96:437-446(1999).
Gregory S.G., Nature 441:315-321(2006).
Selyanko A.A., J. Physiol. (Lond.) 522:349-355(2000).<
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of DFFA using Anti-ICAD (Q177) DFFA Antibody (A03671-3).
Immunohistochemistry (IHC) analyzes of ICAD (Q177) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-ICAD (Q177) DFFA Antibody A03671-3
Western blot (WB) analysis of IDCAD (Q177) polyclonal antibody at 1:500 dilutionLane1:C6 whole cell lysate(40ug)
Lane2:CT26 whole cell lysate(40ug)
Lane3:HCT116 whole cell lysate(40ug)
Lane4:COS-7 whole cell lysate(40ug)
Lane5:SGC7901 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For DFFA (Source: Uniprot.org, NCBI)
Gene Name
DFFA
Full Name
DNA fragmentation factor subunit alpha
Weight
36.522kDa
Alternative Names
DFF1; DFF1DNA fragmentation factor 45 kDa subunit; DFF45; DFF45DNA fragmentation factor subunit alpha; DFF-45DNA fragmentation factor, 45 kD, alpha polypeptide; DFFA; DNA fragmentation factor, 45kDa, alpha polypeptide; ICAD; Inhibitor of CAD DFFA DFF-45, DFF1, ICAD DNA fragmentation factor subunit alpha DNA fragmentation factor subunit alpha|DFF45|DNA fragmentation factor 45 kDa subunit|DNA fragmentation factor, 45kDa, alpha polypeptide|inhibitor of CAD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DFFA, check out the DFFA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DFFA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ICAD (Q177) DFFA Antibody (A03671-3)
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No publications found for A03671-3
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