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Product Info Summary
| SKU: | A31846-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-ICT1 Antibody (C-term)
SKU/Catalog Number
A31846-1
Size
80 µl
Description
Boster Bio Anti-ICT1 Antibody (C-term) (Catalog # A31846-1). Tested in IHC-P, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ICT1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31846-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ICT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-179 amino acids from the C-terminal region of human ICT1.
Reactive Species
A31846-1 is reactive to MRPL58 in Human
Calculated molecular weight
23.6 kDa
Background of MRPL58
Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A31846-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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ICT1 Antibody (C-term) western blot analysis in Hela,Jurkat,U251,MDA-MB453,NCI-H292,K562,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ICT1 antibody detected the ICT1 protein (arrow).
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Immunohistochemical analysis of paraffin-embedded H. colorectal carcinoma section using ICT1 Antibody (C-term). A31846-1 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H. skin section using ICT1 Antibody (C-term). A31846-1 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Specific Publications For Anti-ICT1 Antibody (C-term) (A31846-1)
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