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Product Info Summary
| SKU: | PA1374 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Insulin-like growth factor I IGF1 Antibody
View all IGF-I/IGF-1 Antibodies
SKU/Catalog Number
PA1374
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Insulin-like growth factor I IGF1 Antibody catalog # PA1374. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Insulin-like growth factor I IGF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1374)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human IGF-1, different from the mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1374 is reactive to IGF1 in Human, Mouse, Rat
Applications
PA1374 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
21.841kDa
Background of IGF-I/IGF-1
Insulin-like growth factor 1 (IGF-1) also known as somatomedin C or mechano growth factor is a protein that in humans is encoded by the IGF1 gene. IGF-1 is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7649 daltons.Justice et al. (1990) placed the mouse IGF1 gene on chromosome 10.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-IGF-1 antibody, PA1374, Western blotting
WB: Recombinant Human IGF-1 Protein 2.5ng
Click image to see more details
Anti-IGF-1 antibody, PA1374, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For IGF1 (Source: Uniprot.org, NCBI)
Gene Name
IGF1
Full Name
Insulin-like growth factor I
Weight
21.841kDa
Superfamily
insulin family
Alternative Names
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C IGF1 IGF, IGF-I, IGFI, MGF insulin like growth factor 1 insulin-like growth factor I|insulin-like growth factor 1 (somatomedin C)|insulin-like growth factor IB|mechano growth factor|somatomedin-C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGF1, check out the IGF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Insulin-like growth factor I IGF1 Antibody (PA1374)
Hello CJ!
PA1374 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Peng MF,Tian S,Song YG,Li CX,Miao MS,Zhen-Ren,Li M.Effects of total flavonoids from Eucommia ulmoides Oliv. leaves on polycystic ovary syndrome with insulin resistance model rats induced by letrozole combined with a high-fat diet.J Ethnopharmacol.2021 Feb
Species: Rat
PA1374 usage in article: APP:IHC, SAMPLE:OVARY TISSUE, DILUTION:NA
Elucidating a molecular mechanism that the deterioration of porcine meat quality responds to increased cortisol based on transcriptome sequencing
Effects of the IGF-1/PTEN/Akt/FoxO signaling pathway on male reproduction in rats subjected to water immersion and restraint stress
Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes
Treating Human Meniscal Fibrochondrocytes with hIGF-1 Gene by Liposome
Let-7a inhibits migration of melanoma cells via down-regulation of HMGA2 expression
Li X, Zhang Z, Li Y, Zhao Y, Zhai W, Yang L, Kong D, Wu C, Chen Z, Teng CB. Sci Rep. 2017 Mar 23;7:45002. doi: 10.1038/srep45002. miR-18a counteracts AKT and ERK activation to inhibit the proliferation of pancreatic progenitor cells.
Tang H, Liao Y, Xu L, Zhang C, Liu Z, Deng Y, Jiang Z, Fu S, Chen Z, Zhou S. Int J Cancer. 2013 Nov 15;133(10):2473-82. Doi: 10.1002/Ijc.28262. Epub 2013 Jun 12. Estrogen And Insulin-Like Growth Factor 1 Synergistically Promote The Development Of ...
Huang P, Zhou Z, Wang H, Wei Q, Zhang L, Zhou X, Hutz Rj, Shi F. Int J Mol Med. 2012 Sep;30(3):650-8. Doi: 10.3892/Ijmm.2012.1041. Epub 2012 Jun 20. Effect Of The Igf-1/Pten/Akt/Foxo Signaling Pathway On The Development And Healing Of ...
Chai H, Guo L, Wang X, Fu Y, Guan J, Tan L, Ren L, Yang K. J Mater Sci Mater Med. 2011 Nov;22(11):2525-35. Doi: 10.1007/S10856-011-4427-Z. Epub 2011 Aug 26. Antibacterial Effect Of 317L Stainless Steel Contained Copper In Prevention Of Implant-Rel...
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Insulin-like growth factor I IGF1 Antibody
Question
We are currently using anti-IGF1 antibody PA1374 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The anti-IGF1 antibody (PA1374) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-23
Question
I did not see a band in my samples at 8 kDa. Instead, I saw a very well-defined clear band at about 12.5 kDa. The positive control had a signal at 10 kDa. Does the mouse IGF-1 run higher than the human IGF-1? I used a 15% acrylamide gel.
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
A; There might be problem that your pre-stained markers did not give the required precision on your gel. Note that the nominal molecular weight of mouse IGF-1 is different from that of human.
Boster Scientific Support
Answered: 2019-10-25
Question
How should I reconstitute my WB control (IGF-1 recombinant protein 100 ng/vial) for working with the mouse muscle tissue? I have tried a couple of different dilutions already. It appeared that much of the protein stayed lodged at the top of the gel and did not migrate (Evidence from the signal saturation at the top of the blot in the control lane).
Verified Customer
Verified customer
Asked: 2016-10-04
Answer
We suggest you use 50 µL water to reconstitute with the 100 ng recombinant protein. Then mix 10 µL of this solution with 10 µL of 2X sample buffer so that the protein concentration is 1 ng/µl (i.e., 1 µg/mL).
Boster Scientific Support
Answered: 2016-10-04
