Anti-IGF1/Igf I Rabbit Monoclonal Antibody

IGF-I/IGF-1 antibody

Boster Bio Anti-IGF1/Igf I Rabbit Monoclonal Antibody catalog # M00148. Tested in WB application. This antibody reacts with recombinant protein. Cited in 1 publication(s).

Product Info Summary

SKU: M00148
Size: 100 μl
Reactive Species: Recombinant protein
Host: Rabbit
Application: WB

Product Name

Anti-IGF1/Igf I Rabbit Monoclonal Antibody

View all IGF-I/IGF-1 Antibodies

SKU/Catalog Number

M00148

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-IGF1/Igf I Rabbit Monoclonal Antibody catalog # M00148. Tested in WB application. This antibody reacts with recombinant protein.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IGF1/Igf I Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00148)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DBG-9

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human IGF1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00148 is reactive to IGF1 in Recombinant protein

Applications

M00148 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

95 kDa

Calculated molecular weight

21.841kDa

Background of IGF-I/IGF-1

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For IGF1 (Source: Uniprot.org, NCBI)

Gene Name

IGF1

Full Name

Insulin-like growth factor I

Weight

21.841kDa

Superfamily

insulin family

Alternative Names

IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C IGF1 IGF, IGF-I, IGFI, MGF insulin like growth factor 1 insulin-like growth factor I|insulin-like growth factor 1 (somatomedin C)|insulin-like growth factor IB|mechano growth factor|somatomedin-C

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IGF1, check out the IGF1 Infographic

IGF1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IGF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00148 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Treating Human Meniscal Fibrochondrocytes with hIGF-1 Gene by Liposome

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6 Customer Q&As for Anti-IGF1/Igf I Rabbit Monoclonal Antibody

Question

Does your company have information of complementary-determining region (CDR) of M00148?

Verified customer

Asked: 2021-02-27

Answer

Unfortunately, we don't have the information of complementary-determining region (CDR) of the Anti-IGF1/Igf I Rabbit Monoclonal Antibody (M00148).

Boster Scientific Support

Answered: 2021-03-02

Question

Do you have information regarding the binding site or epitope M00148 may binds to?

Verified customer

Asked: 2021-02-17

Answer

We have not done epitope mapping with the Anti-IGF1/Igf I Rabbit Monoclonal Antibody (M00148). The immunogen sequence for M00148 is RLEMYCAPLKPAKSA.

Boster Scientific Support

Answered: 2021-02-20

Question

Is this M00148 anti-IGF1/Igf I Rabbit Monoclonal antibody reactive to the isotypes of IGF1?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

The immunogen of M00148 anti-IGF1/Igf I Rabbit Monoclonal antibody is A synthesized peptide derived from human IGF1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-19

Question

I have attached the WB image, lot number and protocol we used for brain using anti-IGF1/Igf I Rabbit Monoclonal antibody M00148. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-04-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-04-03

Question

We are interested in to test anti-IGF1/Igf I Rabbit Monoclonal antibody M00148 on human brain for research purposes, then I may be interested in using anti-IGF1/Igf I Rabbit Monoclonal antibody M00148 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-04-02

Answer

The products we sell, including anti-IGF1/Igf I Rabbit Monoclonal antibody M00148, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-04-02

Question

Is a blocking peptide available for product anti-IGF1/Igf I Rabbit Monoclonal antibody (M00148)?

P. Gonzalez

Verified customer

Asked: 2015-02-24

Answer

We do provide the blocking peptide for product anti-IGF1/Igf I Rabbit Monoclonal antibody (M00148). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-02-24

Order DetailsPrice
M00148

100μL

$370
M00148-30ul

30μL sample

$99
M00148-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M00148
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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