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Anti-IGFBP3 Antibody Picoband™
Boster Bio Anti-IGFBP3 Antibody Picoband™ catalog # A00435-3. Tested in ELISA, WB applications. This antibody reacts with Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IGFBP3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00435-3)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived rat IGFBP3 recombinant protein (Position: A29-K268).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross-reactivity with other proteins.
A00435-3 is reactive to Igfbp3 in Rat
A00435-3 is guaranteed for ELISA, WB Boster Guarantee
Background of IGFBP-3
IGFBP3, Insulin-like growth fator-binding protein 3, is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. IGFBP3 is located on chromosome 7. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat
Direct ELISA, 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (A00435-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (Catalog # A00435-3) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP3 at approximately 40KD. The expected band size for IGFBP3 is at 32KD.
Gene/Protein Information For Igfbp3 (Source: Uniprot.org, NCBI)
Insulin-like growth factor-binding protein 3
acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; IBP-3; IBP3BP-53; IGF-binding protein 3; IGFBP3; IGFBP-3; insulin-like growth factor binding protein 3; insulin-like growth factor-binding protein 3 IGFBP3 BP-53, IBP3 insulin like growth factor binding protein 3 insulin-like growth factor-binding protein 3|IBP-3|IGF-binding protein 3|IGFBP-3|acid stable subunit of the 140 K IGF complex|binding protein 29|binding protein 53|growth hormone-dependent binding protein*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on Igfbp3, check out the Igfbp3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Igfbp3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
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