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Product Info Summary
| SKU: | M00124 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Mouse |
| Host: | Rat |
| Application: | WB |
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Product info
Product Name
Anti- IL-18 Monoclonal Antibody
SKU/Catalog Number
M00124
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti- IL-18 Monoclonal Antibody catalog # M00124. Tested in WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti- IL-18 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00124)
Host
Rat
Contents
PBS, pH 7.0. Contains no stabilizers or preservatives
Clonality
Monoclonal
Clone Number
AGOO-9
Isotype
Rat IgG2a, κ
Immunogen
Not available or unknown
Cross-reactivity
No cross reactivity with other proteins
Reactive Species
M00124 is reactive to Il18 in Mouse
Observed Molecular Weight
22 kDa
Calculated molecular weight
22.1 kDa
Background of Il18
Interleukin-18 also known as IL18 is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine.IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-gamma (IFN-gamma) or type II interferon that plays an important role in activating the macrophages or other cells.The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. The human interleukin 18 gene IL18 maps to 11q22.2-q22.3, closely linked to the DRD2 gene locus and distinct from mapped IDDM loci.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00124 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse
Validation Images & Assay Conditions
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Western blot analysis of IL-18 using anti-IL-18 antibody (M00124).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant mouse IL-18 protein 15ng,
Lane 2: recombinant mouse IL-18 protein 10ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rat anti-IL-18 antigen affinity purified monoclonal antibody (Catalog # M00124) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
Specific Publications For Anti- IL-18 Monoclonal Antibody (M00124)
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