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Product Info Summary
| SKU: | A09203 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-IL-1RL2 Antibody
SKU/Catalog Number
A09203
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IL-1RL2 Antibody (Catalog # A09203). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
IL-1RL2 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-IL-1RL2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09203)
Host
Rabbit
Contents
IL-1RL2 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
IL-1RL2 antibody was raised against a 16 amino acid peptide near the center of human IL-1RL2. The immunogen is located within amino acids 260 - 310 of IL-1RL2.
Cross-reactivity
IL-1RL2 antibody is human, mouse and rat reactive. At least three isoforms of IL-1RL2 are known to exist; this antibody will detect all three isoforms. IL-1RL2 antibody is predicted to not cross-react with IL-1R or IL-1RL1.
Reactive Species
A09203 is reactive to IL1RL2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
65.4 kDa
Background of IL1RL2
IL-1RL2 is a member of the interleukin 1 receptor family, but it is incapable of binding to interleukin 1 alpha and interleukin 1 beta with high affinity (1). Together with IL-1RAcP, it can bind members of the IL-36 cytokine family, leading to activation of the NF-kappaB pathway (2). IL-1RL2 can also bind to IL-1F10, resulting in a decreased product of Th17 cytokines in response to immunological or LPS challenge, suggesting that one potential role of IL-1RL2 may be to modulate the immune and inflammation response (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09203 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IL-1RL2 antibody can be used for detection of IL-1RL2 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of IL-1RL2 in human small intestine lysate with IL-1RL2 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of IL-1RL2 in human small intestine tissue with IL-1RL2 antibody at 5 μg/mL.
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Immunofluorescence of IL-1RL2 in human small intestine tissue with IL-1RL2 antibody at 20 μg/mL.
Specific Publications For Anti-IL-1RL2 Antibody (A09203)
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