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Product Info Summary
| SKU: | M00113 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ELISA, IP, IHC-P, WB |
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Product info
Product Name
Anti-IL-33 Antibody (Monoclonal, Nessy-1)
SKU/Catalog Number
M00113
Size
100μg/vial
Form
Liquid
Description
Boster Bio Anti-IL-33 Antibody (Monoclonal, Nessy-1) (Catalog# M00113). Tested in ELISA, IHC-P, IP, WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-33 Antibody (Monoclonal, Nessy-1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00113)
Host
Mouse
Contents
Liquid. In PBS.
Clonality
Monoclonal
Clone Number
Nessy-1
Isotype
IgG1κ
Immunogen
Recombinant human IL-33 (aa 112-270)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Weakly cross-reacts with human PDGF receptor β.
Reactive Species
M00113 is reactive to IL33 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
30759 MW
Background of IL-33
Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 superfamily. The IL33 gene maps to chromosome 9p24.1 by using of genomic database analysis. Recombinant mature human IL33 bound to ST2. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell (CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00113 is guaranteed for ELISA, IP, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunoprecipitation (1:100)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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IL-33 was detected in paraffin-embedded sections of human stomach tissues (A), human bronchus tissues (B), human vulva/anal skin tissues (C), human nasopharynx tissues (D), human colorectal cancer tissues (E), human malignant glioma tissues (F) using mouse anti-IL-33 Antigen Affinity purified monoclonal antibody (Catalog # M00113). The immunohistochemical section was developed using SABC method (Catalog # SA1021).
Protein Target Info & Infographic
Gene/Protein Information For IL33 (Source: Uniprot.org, NCBI)
Gene Name
IL33
Full Name
Interleukin-33
Weight
30759 MW
Superfamily
IL-1 family
Alternative Names
Interleukin-33;IL-33;Interleukin-1 family member 11;IL-1F11;Nuclear factor from high endothelial venules;NF-HEV;Interleukin-33 (95-270);Interleukin-33 (99-270);Interleukin-33 (109-270);IL33;C9orf26, IL1F11, NFHEV; Il33|9230117N10Rik, Il-, Il-33, Il1f, Il1f11, NF-H, NF-HEV|interleukin 33|interleukin-33|nuclear factor from high endothelial venules
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL33, check out the IL33 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL33: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-33 Antibody (Monoclonal, Nessy-1) (M00113)
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No publications found for M00113
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Customer Q&As
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16 Customer Q&As for Anti-IL-33 Antibody (Monoclonal, Nessy-1)
Question
We have been able to see staining in rat brain. Do you have any suggestions? Is anti-IL-33 antibody (Monoclonal, Nessy-1) supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
From literature brain does express IL33. From Uniprot.org, IL33 is expressed in left coronary artery, endothelial cell, adrenal gland trachea, brain, among other tissues. Regarding which tissues have IL33 expression, here are a few articles citing expression in various tissues:
Adrenal gland, and Trachea, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334
Endothelial cell, Pubmed ID: 12819012
Boster Scientific Support
Answered: 2019-12-31
Question
Will anti-IL-33 antibody (Monoclonal, Nessy-1) M00113 work on canine IHC with endothelial cell?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
Our lab technicians have not tested anti-IL-33 antibody (Monoclonal, Nessy-1) M00113 on canine. You can run a BLAST between canine and the immunogen sequence of anti-IL-33 antibody (Monoclonal, Nessy-1) M00113 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine endothelial cell in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-25
Question
My lab would like to test anti-IL-33 antibody (Monoclonal, Nessy-1) M00113 on rat adrenal gland trachea for research purposes, then I may be interested in using anti-IL-33 antibody (Monoclonal, Nessy-1) M00113 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
The products we sell, including anti-IL-33 antibody (Monoclonal, Nessy-1) M00113, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-28
Question
We are currently using anti-IL-33 antibody (Monoclonal, Nessy-1) M00113 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
H. Walker
Verified customer
Asked: 2019-10-10
Answer
The anti-IL-33 antibody (Monoclonal, Nessy-1) (M00113) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-10
Question
Is there a BSA free version of anti-IL-33 antibody (Monoclonal, Nessy-1) M00113 available?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL-33 antibody (Monoclonal, Nessy-1) M00113 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adrenal gland trachea using anti-IL-33 antibody (Monoclonal, Nessy-1) M00113. Let me know if you need anything else.
T. Bhatt
Verified customer
Asked: 2019-08-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-27
Question
See attached the WB image, lot number and protocol we used for adrenal gland trachea using anti-IL-33 antibody (Monoclonal, Nessy-1) M00113. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-09
Question
Can you help my question with product M00113, anti-IL-33 antibody (Monoclonal, Nessy-1). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00113 anti-IL-33 antibody (Monoclonal, Nessy-1), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-03-12
Question
Is this M00113 anti-IL-33 antibody (Monoclonal, Nessy-1) reactive to the isotypes of IL33?
R. Patel
Verified customer
Asked: 2018-03-05
Answer
The immunogen of M00113 anti-IL-33 antibody (Monoclonal, Nessy-1) is Recombinant human IL-33 (aa 112-270). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-03-05
Question
Is a blocking peptide available for product anti-IL-33 antibody (Monoclonal, Nessy-1) (M00113)?
Verified Customer
Verified customer
Asked: 2018-02-23
Answer
We do provide the blocking peptide for product anti-IL-33 antibody (Monoclonal, Nessy-1) (M00113). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-02-23
Question
I was wanting to use your anti-IL-33 antibody (Monoclonal, Nessy-1) for IHC for rat adrenal gland trachea on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat adrenal gland trachea identification?
Verified Customer
Verified customer
Asked: 2017-08-01
Answer
As indicated on the product datasheet, M00113 anti-IL-33 antibody (Monoclonal, Nessy-1) has been tested for IP, IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat adrenal gland trachea in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-01
Question
Does M00113 anti-IL-33 antibody (Monoclonal, Nessy-1) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Miller
Verified customer
Asked: 2017-01-16
Answer
It shows on the product datasheet, M00113 anti-IL-33 antibody (Monoclonal, Nessy-1) as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-01-16
Question
We bought anti-IL-33 antibody (Monoclonal, Nessy-1) for IP on brain a few years ago. I am using mouse, and We intend to use the antibody for IHC next. We want examining brain as well as endothelial cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
A. Yang
Verified customer
Asked: 2015-11-23
Answer
I have checked the website and datasheets of our anti-IL-33 antibody (Monoclonal, Nessy-1) and it appears that M00113 has been validated on mouse in both IP and IHC. Thus M00113 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-11-23
Question
We were satisfied with the WB result of your anti-IL-33 antibody (Monoclonal, Nessy-1). However we have observed positive staining in adrenal gland trachea nucleus using this antibody. Is that expected? Could you tell me where is IL33 supposed to be expressed?
C. Miller
Verified customer
Asked: 2015-04-23
Answer
From literature, adrenal gland trachea does express IL33. Generally IL33 expresses in nucleus. Regarding which tissues have IL33 expression, here are a few articles citing expression in various tissues:
Adrenal gland, and Trachea, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334
Endothelial cell, Pubmed ID: 12819012
Boster Scientific Support
Answered: 2015-04-23
Question
Does anti-IL-33 antibody (Monoclonal, Nessy-1) M00113 work for IHC with adrenal gland trachea?
V. Mitchell
Verified customer
Asked: 2014-05-22
Answer
According to the expression profile of adrenal gland trachea, IL33 is highly expressed in adrenal gland trachea. So, it is likely that anti-IL-33 antibody (Monoclonal, Nessy-1) M00113 will work for IHC with adrenal gland trachea.
Boster Scientific Support
Answered: 2014-05-22
Question
I see that the anti-IL-33 antibody (Monoclonal, Nessy-1) M00113 works with IHC, what is the protocol used to produce the result images on the product page?
G. Jha
Verified customer
Asked: 2013-03-25
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-03-25
