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Product Info Summary
| SKU: | A01160-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL11 Antibody Picoband®
SKU/Catalog Number
A01160-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL11 Antibody Picoband® catalog # A01160-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL11 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01160-1)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat IL11 recombinant protein (Position: P25-L199).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01160-1 is reactive to Il11 in Mouse, Rat
Observed Molecular Weight
21 kDa
Calculated molecular weight
21.6 kDa
Background of Il11
Interleukin 11 (IL-11) is a protein that in humans is encoded by the IL11 gene. The protein encoded by this gene is a member of the gp130 family of cytokines. This gene is mapped to 19q13.42. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. What's more, IL-11 can stimulate the growth of certain lymphocytes and it has functions in many other tissues, including the brain, gut, testis and bone. In transgenic mice, overexpression of the human IL11 gene resulted in the stimulation of bone formation to increase cortical thickness and strength of long bones.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01160-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat liver tissue, rat kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of IL11 using anti-IL11 antibody (A01160-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL11 antigen affinity purified polyclonal antibody (Catalog # A01160-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL11 at approximately 21KD. The expected band size for IL11 is at 21KD.
Specific Publications For Anti-IL11 Antibody Picoband® (A01160-1)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-IL11 Antibody Picoband®
Question
We have seen staining in rat bone marrow. Do you have any suggestions? Is anti-IL11 antibody supposed to stain bone marrow positively?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
From literature bone marrow does express IL11. From Uniprot.org, IL11 is expressed in adenohypophysis, bone marrow, prostate, among other tissues. Regarding which tissues have IL11 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 1828438
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-13
Question
Our team were satisfied with the WB result of your anti-IL11 antibody. However we have seen positive staining in adenohypophysis secreted using this antibody. Is that expected? Could you tell me where is IL11 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
According to literature, adenohypophysis does express IL11. Generally IL11 expresses in secreted. Regarding which tissues have IL11 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 1828438
Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-15
Question
We are currently using anti-IL11 antibody A01160-1 for rat tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
J. Anderson
Verified customer
Asked: 2018-07-24
Answer
The anti-IL11 antibody (A01160-1) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-24
Question
We bought anti-IL11 antibody for WB on adenohypophysis a few months ago. I am using rat, and We intend to use the antibody for ELISA next. My lab would like examining adenohypophysis as well as prostate in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
O. Jha
Verified customer
Asked: 2016-04-14
Answer
I took a look at the website and datasheets of our anti-IL11 antibody and it seems that A01160-1 has been tested on rat in both WB and ELISA. Thus A01160-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-04-14
