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Product Info Summary
| SKU: | A03838 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Inhibin beta-B Antibody
View all Activin B/Inhibin beta B Antibodies
SKU/Catalog Number
A03838
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Inhibin beta-B Antibody catalog # A03838. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Inhibin beta-B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03838)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human NR2F1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03838 is reactive to INHBB in Human, Mouse, Rat
Applications
A03838 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
45.122kDa
Background of Activin B/Inhibin beta B
Hypoxia-inducible factor 3 alpha, also called MOP7, is a protein that in humans is encoded by the HIF3A gene. HIF3A is mapped to 19q13.32. This gene involved in adaptive response to hypoxia. HIF3A suppresses hypoxia-inducible expression of HIF1A and EPAS1. It binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. This gene functions as an inhibitor of angiogenesis in the cornea.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC-P, 1:100-1:300
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HeLa cells using Inhibin beta-B Polyclonal antibody.
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Western Blot (WB) analysis of Mouse Heart and Mouse Brain using Inhibin beta-B antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Skin, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Skin, antibody was diluted at 1:100.
Protein Target Info & Infographic
Gene/Protein Information For INHBB (Source: Uniprot.org, NCBI)
Gene Name
INHBB
Full Name
Inhibin beta B chain
Weight
45.122kDa
Superfamily
TGF-beta family
Alternative Names
activin AB beta polypeptide; Activin B; Activin beta-B chain; INHBB; inhibin beta B chain; inhibin, beta B (activin AB beta polypeptide); inhibin, beta B; Inhibin, beta-2 INHBB inhibin subunit beta B inhibin beta B chain|Inhibin, beta-2|activin AB beta polypeptide|activin beta-B chain|inhibin beta B subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on INHBB, check out the INHBB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for INHBB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Inhibin beta-B Antibody (A03838)
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No publications found for A03838
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Inhibin beta-B Antibody
Question
Is a blocking peptide available for product anti-Inhibin beta-B antibody (A03838)?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
We do provide the blocking peptide for product anti-Inhibin beta-B antibody (A03838). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-06
Question
Can you help my question with product A03838, anti-Inhibin beta-B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03838 anti-Inhibin beta-B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-15
Question
We are currently using anti-Inhibin beta-B antibody A03838 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-12
Answer
The anti-Inhibin beta-B antibody (A03838) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-12
Question
Does A03838 anti-Inhibin beta-B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Lewis
Verified customer
Asked: 2016-08-04
Answer
As indicated on the product datasheet, A03838 anti-Inhibin beta-B antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-08-04
