Anti-Integrin Beta 5 (V746) ITGB5 Antibody

Product Name

Anti-Integrin Beta 5 (V746) ITGB5 Antibody

View all Integrin beta 5 Antibodies

SKU/Catalog Number

A04201

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Integrin Beta 5 (V746) ITGB5 Antibody catalog # A04201. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Integrin Beta 5 (V746) ITGB5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04201)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human E2F7 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04201 is reactive to ITGB5 in Human, Mouse, Rat

Applications

A04201 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

88.054kDa

Background of Integrin beta 5

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L10E family of ribosomal proteins. It is located in the cytoplasm. In vitro studies have shown that the chicken protein can bind to c-Jun and can repress c-Jun-mediated transcriptional activation, but these activities have not been demonstrated in vivo. This gene was initially identified as a candidate for a Wilms tumor suppressor gene, but later studies determined that this gene is not involved in the suppression of Wilms tumor. This gene has been referred to as 'laminin receptor homolog' because a chimeric transcript consisting of sequence from this gene and sequence from the laminin receptor gene was isolated; however, it is not believed that this gene encodes a laminin receptor. Alternative splicing results in multiple transcript variants. This gene also uses multiple polyA signals, with the 3'-most polyA signal overlapping the deoxyribonuclease I-like 1 gene on the opposite strand. This gene is co-transcribed with the small nucleolar RNA gene U70, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Dowdy S.F., Nucleic Acids Res. 19:5763-5769(1991).
van den Ouweland A.M.W., Hum. Mol. Genet. 1:269-273(1992).
Kaneko K., Hum. Mol. Genet. 1:529-533(1992).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-Integrin Beta 5 (V746) ITGB5 Antibody A04201

Western blot (WB) analysis of Integrin β5 polyclonal antibody at 1:500 dillution
Lane1:MCF-7 whole cell lysate(40μg)
Lane2:LO2 whole cell lysate(40μg)
Lane3:CT-26 whole cell lysate(40μg)
Lane4:H9C2 whole cell lysate(40μg)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For ITGB5 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ26658; Integrin beta 5; integrin beta-5; integrin, beta 5; ITGB5 ITGB5 integrin subunit beta 5 integrin beta-5|testis secretory sperm-binding protein Li 217p

For more info on ITGB5, check out the ITGB5 Infographic

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In this infographic, you will see the following information for ITGB5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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