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- Table of Contents
NLRC4 antibody (A00525) performs well in ELISA, IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of Ipaf in THP1 cells with Ipaf antibody at 10 μg/mL.
Designing a Western blot for NLRC4? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the NLRC4 Western Blot GuideBundle Save
Anti-Ipaf NLRC4 Antibody
NLRC4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IF, ICC, WB |
Recommend Dilution
Application
Dilution
Species
Ipaf polyclonal antibody can be used for the detection of Ipaf by Western blot at 1 and 2 μg/mL. A 110 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Ipaf NLRC4 Antibody
View all NLRC4 Antibodies
View all NLRC4 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Ipaf antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
Ipaf Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Ipaf antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of human Ipaf. The immunogen is located within the last 50 amino acids of Ipaf.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
116.2 kDa
Background of NLRC4
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adaptor molecules and proteases including several members of the caspase family. Another family of proteins that functions as a critical regulator of apoptosis and NFκ signaling pathways is the CED-4/Apaf-1 (apoptosis protein activating factor-1) protein family. Ipaf (ICE protease activating factor) is a CED-4/Apaf-1 family member that activates caspase-1/ICE and can induce apoptosis in human cells in a caspase-1 dependent manner. Ipaf and caspase-1 are thought to interact with each other through the association of the Ipaf amino-terminal CARD (caspase recruitment domain) and amino-terminal CARD of caspase-1.
Cite This Product
Anti-Ipaf NLRC4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00525)
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Product Promise
We promise Anti-Ipaf NLRC4 Antibody performs excellently in relevant applications for ELISA, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00525
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 31, 2026