Click image to see more details
Product Info Summary
| SKU: | M01603 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IQGAP1 Monoclonal Antibody
SKU/Catalog Number
M01603
BM5447 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IQGAP1 Monoclonal Antibody catalog # M01603. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IQGAP1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01603)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADGG-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IQGAP1 Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane.
Reactive Species
M01603 is reactive to IQGAP1 in Human, Mouse
Observed Molecular Weight
180 kDa
Calculated molecular weight
189.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01603 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of IQGAP1 expression in HeLa cell lysate.
Specific Publications For Anti-IQGAP1 Monoclonal Antibody (M01603)
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Customer Q&As
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4 Customer Q&As for Anti-IQGAP1 Monoclonal Antibody
Question
Will anti-IQGAP1 Monoclonal antibody M01603 work for IF with bone marrow?
L. Collins
Verified customer
Asked: 2020-04-03
Answer
According to the expression profile of bone marrow, IQGAP1 is highly expressed in bone marrow. So, it is likely that anti-IQGAP1 Monoclonal antibody M01603 will work for IF with bone marrow.
Boster Scientific Support
Answered: 2020-04-03
Question
I see that the anti-IQGAP1 Monoclonal antibody M01603 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-IQGAP1 Monoclonal antibody M01603. Let me know if you need anything else.
T. Walker
Verified customer
Asked: 2018-01-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-02
Question
Is this M01603 anti-IQGAP1 Monoclonal antibody reactive to the isotypes of IQGAP1?
D. Johnson
Verified customer
Asked: 2017-02-23
Answer
The immunogen of M01603 anti-IQGAP1 Monoclonal antibody is A synthesized peptide derived from human IQGAP1 Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-02-23
