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Product Info Summary
| SKU: | M01247 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IRAK4 Monoclonal Antibody
SKU/Catalog Number
M01247
BM5188 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IRAK4 Monoclonal Antibody catalog # M01247. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRAK4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01247)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABIH-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IRAK4 Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
Reactive Species
M01247 is reactive to IRAK4 in Human
Observed Molecular Weight
52 kDa
Calculated molecular weight
51.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01247 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of IRAK4 expression in Jurkat cell lysate.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
Specific Publications For Anti-IRAK4 Monoclonal Antibody (M01247)
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1 Customer Q&As for Anti-IRAK4 Monoclonal Antibody
Question
What is the sample target on Western blot analysis in your product image for M01247?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-IRAK4 Monoclonal Antibody (M01247) expression in Jurkat cell lysate.
Boster Scientific Support
Answered: 2019-12-10
