Anti-ITK Monoclonal Antibody

ITK antibody

Boster Bio Anti-ITK Monoclonal Antibody catalog # M01385. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M01385
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IHC, WB

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Product Name

Anti-ITK Monoclonal Antibody

View all ITK Antibodies

SKU/Catalog Number

M01385

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ITK Monoclonal Antibody catalog # M01385. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ITK Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01385)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACAB-9

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ITK.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01385 is reactive to ITK in Human

Applications

M01385 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

71.831kDa

Background of ITK

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
FC 1:60

Validation Images & Assay Conditions

Gene/Protein Information For ITK (Source: Uniprot.org, NCBI)

Gene Name

ITK

Full Name

Tyrosine-protein kinase ITK/TSK

Weight

71.831kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.10; EC 2.7.10.2; EMT; EMTMGC126257; homolog of mouse T-cell itk/tsk; IL2-inducible T-cell kinase; ITK; Kinase EMT; LYK; LYKMGC126258; PSCTK2; T-cell-specific kinase; Tcsk; tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk ITK EMT, LPFS1, LYK, PSCTK2 IL2 inducible T cell kinase tyrosine-protein kinase ITK/TSK|IL-2-inducible T-cell kinase|T-cell-specific kinase|homolog of mouse T-cell itk/tsk|interleukin-2-inducible T-cell kinase|kinase EMT|tyrosine-protein kinase LYK

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ITK, check out the ITK Infographic

ITK infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ITK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-ITK Monoclonal Antibody

Question

What is the sample target on Western blot analysis in your product image for M01385?

Verified customer

Asked: 2019-12-09

Answer

Western blot analysis of the Anti-ITK Monoclonal Antibody (M01385) expression in Jurkat cell lysate.

Boster Scientific Support

Answered: 2019-12-10

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-ITK Monoclonal antibody M01385. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-14

Question

Is a blocking peptide available for product anti-ITK Monoclonal antibody (M01385)?

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

We do provide the blocking peptide for product anti-ITK Monoclonal antibody (M01385). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-23

Question

What is the immunogen sequence of M01385? Would this antibody target the protein aa395 - 497 region?

Verified customer

Asked: 2019-09-16

Answer

The immunogen sequence of the Anti-ITK Monoclonal Antibody (M01385) is aa1-17 MNNFILLEEQLIKKSQC.

Boster Scientific Support

Answered: 2019-09-17

Question

Do you have a BSA free version of anti-ITK Monoclonal antibody M01385 available?

Verified Customer

Verified customer

Asked: 2019-01-14

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ITK Monoclonal antibody M01385 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-01-14

Question

Does M01385 anti-ITK Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-04-17

Answer

It shows on the product datasheet, M01385 anti-ITK Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-04-17

Question

Does anti-ITK Monoclonal antibody M01385 work for WB with thymus?

E. Jones

Verified customer

Asked: 2018-02-07

Answer

According to the expression profile of thymus, ITK is highly expressed in thymus. So, it is likely that anti-ITK Monoclonal antibody M01385 will work for WB with thymus.

Boster Scientific Support

Answered: 2018-02-07

Order DetailsPrice
M01385

100μL

$370
M01385-30ul

30μL sample

$99
M01385-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M01385
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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