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- Table of Contents
JAK2 antibody (M00027) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 13 Publications
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IHC analysis of JAK2 using anti-JAK2 antibody (M00027).
JAK2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-JAK2 Antibody (M00027) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
JAK2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-JAK2 Antibody (M00027) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-JAK2 Monoclonal Antibody
JAK2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
JAK2 antibody was positively detected by WB in:
human HepG2 whole cell, human THP-1 whole cell, rat NRK whole cell, rat PC-12 whole cell, mouse RAW2647 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
JAK2 antibody was positively detected by IHC in:
human prostate cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
JAK2 antibody was positively detected by ICC/IF in:
A549 cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50-100
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-JAK2 Monoclonal Antibody
View all JAK2 Antibodies
View all JAK2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
COG-10
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human JAK2 Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2.
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
131 kDa
Calculated Molecular Weight
130.7 kDa
Cite This Product
Anti-JAK2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00027)
Protocols
Western Blot protocol for JAK2 antibody
Immunohistochemistry (IHC) protocol for JAK2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for JAK2 antibody
Publications
Application:
Species:
CCL2 knockdown attenuates post-traumatic external cerebral herniation by suppressing JAK-STAT signaling following traumatic brain injury
Published: 2026-08-14
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 5.60
Species: Mouse
PMID: 42600269
FXR-mediated transcriptional regulation of KLF11 mitigates contrast-induced acute kidney injury via suppressing JAK2/STAT3 pathway
Published: 2026-03-18
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 4.70
Species: Human
PMID: 41855776
A novel polysaccharide from Polygonatum cyrtonema fibrous root mediates anti-diarrheal effects by reprogramming macrophage M1/M2 polarization
Published: 2026-03-16
Journal: Journal of Functional Foods
Application: WB
Impact Factor: 4.00
Species: Mouse
METTL3 modulates bone Cancer pain via m6A-dependent JAK2/STAT3 activation in spinal astrocytes
Published: 2025-08-03
Journal: CELLULAR SIGNALLING
Application: WB
Impact Factor: 3.70
Species: Mouse
PMID: 40752537
Engineered Panax notoginseng polysaccharide micelles inhibit macrophage polarization and delay the progression of rheumatoid arthritis via JAK2-STAT3 signaling pathway
Published: 2025-07-14
Journal: JOURNAL OF NANOBIOTECHNOLOGY
Application: WB
Impact Factor: 12.60
Species: Rat,Mouse
PMID: 40660236
Sappanone A attenuates rheumatoid arthritis via inhibiting PI3K/AKT/NF-κB and JAK2/STAT3 signaling pathways in vivo and in vitro
Published: 2024-11-12
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Human,Rat
PMID: 39520962
SO2 activates Th17 cells through the JAK1,2/STAT3 signaling pathway
Published: 2024-09-30
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Human
PMID: 39353391
In situ formed reactive oxygen species-responsive dipyridamole prodrug hydrogel: Spatiotemporal drug delivery for chemoimmunotherapy
Published: 2024-09-20
Journal: JOURNAL OF CONTROLLED RELEASE
Application: WB
Impact Factor: 10.50
Species: Mouse
PMID: 39216598
Inhibition of JAK/STAT3 Expression by Acute Myeloid Leukemia-Targeted Nanoliposome for Chemotherapy Enhancement
Published: 2024-08-28
Journal: ACS Omega
Application: WB
Impact Factor: 3.70
Species: Mouse
PMID: 39281932
Curcumin and its nano-formulations: Defining triple-negative breast cancer targets through network pharmacology, molecular docking, and experimental verification
Published: 2022-08-08
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 5.99
Species: Human
PMID: 36003508
Water extract of sporoderm-broken spores of Ganoderma lucidum enhanced pd-l1 antibody efficiency through downregulation and relieved complications of pd-l1 monoclonal antibody
Published: 2020-09-18
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 4.55
Species: Mouse,Human
PMID: 33152901
ELP2 negatively regulates osteoblastic differentiation impaired by tumor necrosis factor α in MC3T3–E1 cells through STAT3 activation
Published: 2019-03-07
Journal: JOURNAL OF CELLULAR PHYSIOLOGY
Application: WB
Impact Factor: 4.52
Species: Mouse
PMID: 30847950
Retracted: Hispidulin suppresses cell growth and metastasis by targeting PIM1 through JAK2/STAT3 signaling in colorectal cancer
Published: 2018-03-25
Journal: CANCER SCIENCE
Application: WB
Impact Factor: 4.37
Species: Human
PMID: 29575334
Review
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2 Reviews For Anti-JAK2 Monoclonal Antibody
Anti-JAK2 Antibody (M00027) reliably detected reduced JAK2 abundance in liver tissue from ANIT-induced cholestatic rats by WB, with only faint non-specific bands present.
| SKU | M00027 |
|---|---|
| Application | Western Blot |
| Sample | Liver tissue from an ANIT-induced rat model |
| Sample Processing Description | Cholestatic liver injury was induced in SD rats via intragastric administration of ANIT. Liver tissues were harvested 48 hours later, and proteins were extracted using RIPA lysis buffer. Following BCA quantification, the samples were mixed with loading buffer and boiled for denaturation. |
| Other Reagents | Blocking buffer, RIPA lysis buffer |
| Primary Antibody | JAK2 Monoclonal Antibody |
| Primary Incubation | 1:2,000 dilution, incubated overnight at 4°C. |
| Secondary Antibody | HRP-conjugated anti-rabbit IgG secondary antibody |
| Secondary Incubation | 1:50000 |
| Detection | ECL, Imaging system: Contact-Based Non-Destructive Signal Imaging |
| Results Summary | The image shows the following lanes: Lane 1 represents the liver of a control group rat, displaying a clear and uniform JAK2 band; Lane 2 represents the ANIT-treated group; and Lane 3 represents the drug-treated group, where the JAK2 band is markedly reduced. This demonstrates that the antibody can effectively detect changes in JAK2 expression. |
Yin Zhou, The Fifth Medical Center of Chinese PLA General Hospital
Verified customer
Submitted 2026-09-16
This antibody is highly efficient and specific, suitable for Western blot detection of JAK2 protein in rat colon tissue, with only minor nonspecific bands.
| SKU | M00027 |
|---|---|
| Application | Western Blot |
| Sample | rat colon tissue |
| Sample Processing Description | RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE. |
| Other Reagents | Blocking buffer |
| Primary Antibody | JAK2 Monoclonal Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1:5000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | The figure shows the Western blot results of the target protein JAK2 and the internal control Actin in rat colon tissue across the following groups: normal, disease model, low/middle/high dose traditional Chinese medicine treatment, and Western medicine treatment. The target bands are clear and well defined, and the experimental results are satisfactory. |
Shiyu Zhang, LUTCM
Verified customer
Submitted 2026-01-06
Product Promise
We promise Anti-JAK2 Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00027
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026