Product Info Summary
SKU: | M02608 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, WB |
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Product info
Product Name
Anti-JNK1/JNK3 MAPK8 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02608
BM4805 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-JNK1/JNK3 MAPK8 Rabbit Monoclonal Antibody catalog # M02608. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-JNK1/JNK3 MAPK8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02608)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ICC-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human JNK1/JNK3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02608 is reactive to MAPK8 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
290 kDa
Calculated molecular weight
48296 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02608 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IP 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of JNK1/JNK3 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MAPK8 (Source: Uniprot.org, NCBI)
Gene Name
MAPK8
Full Name
Mitogen-activated protein kinase 8
Weight
48296 MW
Superfamily
protein kinase superfamily
Alternative Names
Mitogen-activated protein kinase 8;MAP kinase 8;MAPK 8;2.7.11.24;JNK-46;Stress-activated protein kinase 1c;SAPK1c;Stress-activated protein kinase JNK1;c-Jun N-terminal kinase 1;MAPK8;JNK1, PRKM8, SAPK1, SAPK1C; MAPK8 JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c mitogen-activated protein kinase 8 mitogen-activated protein kinase 8|JUN N-terminal kinase|MAP kinase 8|c-Jun N-terminal kinase 1|stress-activated protein kinase 1|stress-activated protein kinase 1c
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAPK8, check out the MAPK8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAPK8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JNK1/JNK3 MAPK8 Rabbit Monoclonal Antibody (M02608)
Hello CJ!
M02608 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of endoplasmic reticulum stress involved in manganese‑induced neurotoxicity in rats
Therapeutic effects of 1,25-dihydroxyvitamin D3 on diabetes-induced liver complications in a rat model
Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress
Cui Y, Wu W, Lv P, Zhang J, Bai B, Cao W. Oncotarget. 2017 Dec 11;9(1):783-790. doi: 10.18632/oncotarget.23153. eCollection 2018 Jan 2. Down-regulation of long non-coding RNA ESCCAL_1 inhibits tumor growth of esophageal squamous cell carcinoma in ...
Ding Y, Zou J, Li Z, Tian J, Abdelalim S, Du F, She R, Wang D, Tan C, Wang H, Chen W, Lv D, Chang L. Plos One. 2011;6(5):E20008. Doi: 10.1371/Journal.Pone.0020008. Epub 2011 May 23. Study Of Histopathological And Molecular Changes Of Rat Kidney Un...
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-JNK1/JNK3 MAPK8 Rabbit Monoclonal Antibody
Question
We want to test anti-JNK1/JNK3 Rabbit Monoclonal antibody M02608 on mouse forebrain for research purposes, then I may be interested in using anti-JNK1/JNK3 Rabbit Monoclonal antibody M02608 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
The products we sell, including anti-JNK1/JNK3 Rabbit Monoclonal antibody M02608, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-30
Question
Would anti-JNK1/JNK3 Rabbit Monoclonal antibody M02608 work for IP with forebrain?
V. Li
Verified customer
Asked: 2019-12-26
Answer
According to the expression profile of forebrain, MAPK8 is highly expressed in forebrain. So, it is likely that anti-JNK1/JNK3 Rabbit Monoclonal antibody M02608 will work for IP with forebrain.
Boster Scientific Support
Answered: 2019-12-26
Question
We are interested in using your anti-JNK1/JNK3 Rabbit Monoclonal antibody for positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
B. Li
Verified customer
Asked: 2019-11-18
Answer
I appreciate your inquiry. This M02608 anti-JNK1/JNK3 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IP, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-18
Question
We ordered your anti-JNK1/JNK3 Rabbit Monoclonal antibody for IP on brain in the past. I am using rat, and I plan to use the antibody for WB next. I am looking for examining brain as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
I viewed the website and datasheets of our anti-JNK1/JNK3 Rabbit Monoclonal antibody and it appears that M02608 has been validated on rat in both IP and WB. Thus M02608 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-14
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for forebrain using anti-JNK1/JNK3 Rabbit Monoclonal antibody M02608. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-01
Question
We were content with the WB result of your anti-JNK1/JNK3 Rabbit Monoclonal antibody. However we have been able to see positive staining in brain cytoplasm. using this antibody. Is that expected? Could you tell me where is MAPK8 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
From what I have seen in literature, brain does express MAPK8. Generally MAPK8 expresses in cytoplasm. Regarding which tissues have MAPK8 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8654373
Cervix carcinoma, Pubmed ID: 18669648, 18691976
Fetal brain, Pubmed ID: 8137421
Boster Scientific Support
Answered: 2019-08-14
Question
Is this M02608 anti-JNK1/JNK3 Rabbit Monoclonal antibody reactive to the isotypes of MAPK8?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
The immunogen of M02608 anti-JNK1/JNK3 Rabbit Monoclonal antibody is A synthesized peptide derived from human JNK1/JNK3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-16
Question
I see that the anti-JNK1/JNK3 Rabbit Monoclonal antibody M02608 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-30
Question
We have been able to see staining in human fetal brain. Any tips? Is anti-JNK1/JNK3 Rabbit Monoclonal antibody supposed to stain fetal brain positively?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
According to literature fetal brain does express MAPK8. According to Uniprot.org, MAPK8 is expressed in forebrain, fetal brain, brain, cervix carcinoma, among other tissues. Regarding which tissues have MAPK8 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8654373
Cervix carcinoma, Pubmed ID: 18669648, 18691976
Fetal brain, Pubmed ID: 8137421
Boster Scientific Support
Answered: 2019-05-21
Question
Does M02608 anti-JNK1/JNK3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-07
Answer
You can see on the product datasheet, M02608 anti-JNK1/JNK3 Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-07
Question
See below the WB image, lot number and protocol we used for forebrain using anti-JNK1/JNK3 Rabbit Monoclonal antibody M02608. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-02-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-20
Question
My question regarding product M02608, anti-JNK1/JNK3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-04-16
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02608 anti-JNK1/JNK3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-16
Question
Is there a BSA free version of anti-JNK1/JNK3 Rabbit Monoclonal antibody M02608 available?
Verified Customer
Verified customer
Asked: 2017-09-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-JNK1/JNK3 Rabbit Monoclonal antibody M02608 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-28
Question
We are currently using anti-JNK1/JNK3 Rabbit Monoclonal antibody M02608 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-14
Answer
The anti-JNK1/JNK3 Rabbit Monoclonal antibody (M02608) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-14
Question
I was wanting to use your anti-JNK1/JNK3 Rabbit Monoclonal antibody for IP for mouse forebrain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse forebrain identification?
A. Thomas
Verified customer
Asked: 2016-01-26
Answer
As indicated on the product datasheet, M02608 anti-JNK1/JNK3 Rabbit Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse forebrain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-01-26
Question
Is a blocking peptide available for product anti-JNK1/JNK3 Rabbit Monoclonal antibody (M02608)?
W. Carter
Verified customer
Asked: 2014-10-21
Answer
We do provide the blocking peptide for product anti-JNK1/JNK3 Rabbit Monoclonal antibody (M02608). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-10-21