Product Info Summary
SKU: | PA1767 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Kallikrein 6/KLK6 Antibody
View all Kallikrein 6/Neurosin Antibodies
SKU/Catalog Number
PA1767
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Kallikrein 6/KLK6 Antibody catalog # PA1767. Tested in IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kallikrein 6/KLK6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1767)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Kallikrein 6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1767 is reactive to KLK6 in Human
Applications
PA1767 is guaranteed for IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
27 kDa
Calculated molecular weight
26.856kDa
Background of Kallikrein 6/Neurosin
KLK6 (Kallikrein-related peptidase 6), also called KALLIKREIN 6, NEUROSIN, PROTEASE M, ZYME or PRSS9, is a protein that in humans is encoded by the KLK6 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. Northern blot analysis revealed that the PRSS9 mRNA was expressed in several primary tumors and cell lines from mammary, prostate, and ovarian cancers, but was not detected in any metastases of these cancers. The KLK6 gene is mapped on 19q13.41. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Upon cellular stress, neurosin was released from mitochondria to the cytosol, which resulted in the increase of degraded alpha-synuclein species. Neurosin may play a significant role in physiologic alpha-synuclein degradation and also in the pathogenesis of synucleinopathies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-Kallikrein 6 antibody, PA1767, Western blotting
All lanes: Anti Kallikrein 6 (PA1767) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: MM453 Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: SW620 Whole Cell Lysate at 40ug
Lane 9: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 27KD
Click image to see more details
Anti-Kallikrein 6 antibody, PA1767, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Click image to see more details
Anti-Kallikrein 6 antibody, PA1767, ICC
ICC: PC-3 Cell
Protein Target Info & Infographic
Gene/Protein Information For KLK6 (Source: Uniprot.org, NCBI)
Gene Name
KLK6
Full Name
Kallikrein-6
Weight
26.856kDa
Superfamily
peptidase S1 family
Alternative Names
Bssp; EC 3.4.21; EC 3.4.21.-; hK6; kallikrein 6 (neurosin, zyme); Kallikrein 6; kallikrein-6; kallikrein-related peptidase 6; KLK6; Klk7; Neurosin; Protease M; protease, serine, 18; PRSS18; PRSS9; PRSS9MGC9355; Serine protease 18; Serine protease 9; SP59; Zyme KLK6 Bssp, Klk7, PRSS18, PRSS9, SP59, hK6 kallikrein related peptidase 6 kallikrein-6|neurosin|protease M|serine protease 18|serine protease 9|zyme
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLK6, check out the KLK6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLK6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kallikrein 6/KLK6 Antibody (PA1767)
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No publications found for PA1767
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Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
5 Customer Q&As for Anti-Kallikrein 6/KLK6 Antibody
Question
Will anti-Kallikrein 6/KLK6 antibody PA1767 work for IHC with colon?
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
According to the expression profile of colon, KLK6 is highly expressed in colon. So, it is likely that anti-Kallikrein 6/KLK6 antibody PA1767 will work for IHC with colon.
Boster Scientific Support
Answered: 2020-04-15
Question
What is the suggested blocking concentration for western blot using BSA?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
3% BSA for blocking in WB is recommended.
Boster Scientific Support
Answered: 2020-02-21
Question
Does PA1767 anti-Kallikrein 6/KLK6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
O. Anderson
Verified customer
Asked: 2019-12-18
Answer
As indicated on the product datasheet, PA1767 anti-Kallikrein 6/KLK6 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-18
Question
We are currently using anti-Kallikrein 6/KLK6 antibody PA1767 for human tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
The anti-Kallikrein 6/KLK6 antibody (PA1767) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-01
Question
What are some tissues and cell lines recommended for use as negative control?
Verified Customer
Verified customer
Asked: 2015-09-30
Answer
Hepatic cell, lymphocyte, muscle cell and osteocyte may be used as negative control.
Boster Scientific Support
Answered: 2015-09-30