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Product Info Summary
| SKU: | A05366 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-KAPCB PRKACB Antibody
View all PKA C beta Antibodies
SKU/Catalog Number
A05366
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-KAPCB PRKACB Antibody (Catalog # A05366). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KAPCB PRKACB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05366)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human KAPCB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05366 is reactive to PRKACB in Human, Mouse, Rat
Applications
A05366 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
40.623kDa
Background of PKA C beta
Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis.
Beebe S.J., Mol. Endocrinol. 4:465-475(1990).
Gregory S.G., Nature 441:315-321(2006).
Wu K.-J., Oncogene 21:7872-7882(2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from Jurkat cells and COLO205 cells, using KAPCB antibody A05366.
Protein Target Info & Infographic
Gene/Protein Information For PRKACB (Source: Uniprot.org, NCBI)
Gene Name
PRKACB
Full Name
cAMP-dependent protein kinase catalytic subunit beta
Weight
40.623kDa
Superfamily
protein kinase superfamily
Alternative Names
cAMP-dependent protein kinase catalytic beta subunit isoform 4ab; cAMP-dependent protein kinase catalytic subunit beta; cAPKbeta; DKFZp781I2452; EC 2.7.11; EC 2.7.11.11; MGC41879; MGC9320; PKA C beta; PKA C-beta; PKACB; PRKACB; protein kinase A catalytic subunit beta; protein kinase, cAMP-dependent, catalytic, beta PRKACB CAFD2, PKA C-beta, PKACB protein kinase cAMP-activated catalytic subunit beta cAMP-dependent protein kinase catalytic subunit beta|protein kinase A catalytic subunit beta|protein kinase, cAMP-dependent, beta catalytic subunit|protein kinase, cAMP-dependent, catalytic, beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKACB, check out the PRKACB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKACB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KAPCB PRKACB Antibody (A05366)
Hello CJ!
No publications found for A05366
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Anti-KAPCB PRKACB Antibody
Question
Is this A05366 anti-KAPCB antibody reactive to the isotypes of PRKACB?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
The immunogen of A05366 anti-KAPCB antibody is Synthesized peptide derived from internal of human KAPCB. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-06
Question
Will A05366 anti-KAPCB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
You can see on the product datasheet, A05366 anti-KAPCB antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-03
Question
We are currently using anti-KAPCB antibody A05366 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
The anti-KAPCB antibody (A05366) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-11
Question
I was wanting to use your anti-KAPCB antibody for WB for rat testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat testis identification?
C. Krishna
Verified customer
Asked: 2016-09-13
Answer
It shows on the product datasheet, A05366 anti-KAPCB antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-09-13
