Anti-KappaB ras NKIRAS1 Antibody

Product Name

Anti-KappaB ras NKIRAS1 Antibody

View all NKIRAS1 Antibodies

SKU/Catalog Number

A11990

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-KappaB ras NKIRAS1 Antibody (Catalog # A11990). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

KappaB ras antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-KappaB ras NKIRAS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11990)

Host

Rabbit

Contents

KappaB ras Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

KappaB ras antibody was raised against a 18 amino acid synthetic peptide from near the center of human KappaB ras 1. The immunogen is located within amino acids 80 - 130 of KappaB ras.

Reactive Species

A11990 is reactive to NKIRAS1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

21.6 kDa

Background of NKIRAS1

KappaB ras-1 (κB-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IκB (IκB), an inhibitor of the transcription factor NF-κB. IκB exists in two homologous forms, IκB-alpha and IκB-beta, although IκB-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive IκB-alpha-NF-κB complexes can shuttle in and out of the nucleus, IκB-beta-NF-κB complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the IκB-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of IκB-beta and regulating the rate of degradation of IκB-beta. This antibody detects both kappaB-ras1 and kappaB-ras2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A11990 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

KappaB ras antibody can be used for detection of KappaB ras by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information NKIRAS1

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