Product Info Summary
SKU: | A02160 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-KCNJ5/Kir3.4 Antibody
SKU/Catalog Number
A02160
Size
100ul
Form
Liquid
Description
Boster Bio Anti-KCNJ5/Kir3.4 Antibody (Catalog# A02160). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KCNJ5/Kir3.4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02160)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
7A7
Isotype
Rabbit IgG
Immunogen
A peptide derived from human KCNJ5. Immunogen sequence location: 370 - 419
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02160 is reactive to KCNJ5 in Human, Mouse, Rat
Applications
A02160 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
47.668kDa
Background of Kir3.4
Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:5000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from HeLa cells, using KCNJ5 Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Immunofluorescence analysis of A549 cells, using KCNJ5 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For KCNJ5 (Source: Uniprot.org, NCBI)
Gene Name
KCNJ5
Full Name
G protein-activated inward rectifier potassium channel 4
Weight
47.668kDa
Superfamily
inward rectifier-type potassium channel (TC 1.A.2.1) family
Alternative Names
Cardiac inward rectifier; CIRKIR3.4; G protein-activated inward rectifier potassium channel 4; GIRK-4; GIRK4Kir3.4; Heart KATP channel; Inward rectifier K(+) channel Kir3.4; inward rectifier K+ channel KIR3.4; IRK-4; KATP-1; KATP1cardiac ATP-sensitive potassium channel; LQT13; Potassium channel, inwardly rectifying subfamily J member 5; potassium inwardly-rectifying channel, subfamily J, member 5 KCNJ5 CIR, GIRK4, KATP1, KIR3.4, LQT13 potassium inwardly rectifying channel subfamily J member 5 G protein-activated inward rectifier potassium channel 4|IRK-4|cardiac ATP-sensitive potassium channel|heart KATP channel|inward rectifier K+ channel KIR3.4|potassium channel, inwardly rectifying subfamily J, member 5|potassium voltage-gated channel subfamily J member 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KCNJ5, check out the KCNJ5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KCNJ5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KCNJ5/Kir3.4 Antibody (A02160)
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No publications found for A02160
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-KCNJ5/Kir3.4 Antibody
Question
We are currently using anti-KCNJ5/Kir3.4 antibody A02160 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
The anti-KCNJ5/Kir3.4 antibody (A02160) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-26