Anti-KCNJ5/Kir3.4 Antibody

Kir3.4 antibody

Boster Bio Anti-KCNJ5/Kir3.4 Antibody (Catalog# A02160). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.

Product Info Summary

SKU: A02160
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, ICC, WB

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Product Name

Anti-KCNJ5/Kir3.4 Antibody

View all Kir3.4 Antibodies

SKU/Catalog Number

A02160

Size

100ul

Form

Liquid

Description

Boster Bio Anti-KCNJ5/Kir3.4 Antibody (Catalog# A02160). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-KCNJ5/Kir3.4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02160)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

7A7

Isotype

Rabbit IgG

Immunogen

A peptide derived from human KCNJ5. Immunogen sequence location: 370 - 419

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A02160 is reactive to KCNJ5 in Human, Mouse, Rat

Applications

A02160 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

47.668kDa

Background of Kir3.4

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:5000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For KCNJ5 (Source: Uniprot.org, NCBI)

Gene Name

KCNJ5

Full Name

G protein-activated inward rectifier potassium channel 4

Weight

47.668kDa

Superfamily

inward rectifier-type potassium channel (TC 1.A.2.1) family

Alternative Names

Cardiac inward rectifier; CIRKIR3.4; G protein-activated inward rectifier potassium channel 4; GIRK-4; GIRK4Kir3.4; Heart KATP channel; Inward rectifier K(+) channel Kir3.4; inward rectifier K+ channel KIR3.4; IRK-4; KATP-1; KATP1cardiac ATP-sensitive potassium channel; LQT13; Potassium channel, inwardly rectifying subfamily J member 5; potassium inwardly-rectifying channel, subfamily J, member 5 KCNJ5 CIR, GIRK4, KATP1, KIR3.4, LQT13 potassium inwardly rectifying channel subfamily J member 5 G protein-activated inward rectifier potassium channel 4|IRK-4|cardiac ATP-sensitive potassium channel|heart KATP channel|inward rectifier K+ channel KIR3.4|potassium channel, inwardly rectifying subfamily J, member 5|potassium voltage-gated channel subfamily J member 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on KCNJ5, check out the KCNJ5 Infographic

KCNJ5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KCNJ5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-KCNJ5/Kir3.4 Antibody

Question

We are currently using anti-KCNJ5/Kir3.4 antibody A02160 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

The anti-KCNJ5/Kir3.4 antibody (A02160) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-26

Order DetailsPrice
A02160

100 μL

$335

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