Anti-Kcnn4 Antibody Picoband™

Product Name

Anti-Kcnn4 Antibody Picoband™

View all KCNN4 Antibodies

SKU/Catalog Number

A01936-4

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Kcnn4 Antibody Picoband™ catalog # A01936-4. Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Kcnn4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01936-4)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Kcnn4 recombinant protein (Position: Y324-T425).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01936-4 is reactive to KCNN4 in Mouse

Applications

A01936-4 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

48 kDa

Calculated molecular weight

47.696kDa

Background of KCNN4

Intermediate conductance calcium-activated potassium channel protein 1 (KCNN4, Kca3.1) is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. KCNN4 may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse
Direct ELISA, 0.1-0.5 µg/ml, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Kcnn4 using anti-Kcnn4 antibody (A01936-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kcnn4 antigen affinity purified polyclonal antibody (Catalog # A01936-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kcnn4 at approximately 48 kDa. The expected band size for Kcnn4 is at 48 kDa.

Gene/Protein Information For KCNN4 (Source: Uniprot.org, NCBI)

Alternative Names

hIKCa1; hKCa4; hSK4; IK1; IKCa1; intermediate conductance calcium-activated potassium channel protein 4; KCa3.1IKCA1; KCa4; KCA4SKCa4; potassium intermediate/small conductance calcium-activated channel, subfamilyN, member 4; putative erythrocyte intermediate conductance calcium-activated potassiumGardos channel; Putative Gardos channel; SK4SKCa 4 KCNN4 DHS2, IK, IK1, IKCA1, KCA4, KCa3.1, SK4, hIKCa1, hKCa4, hSK4 potassium calcium-activated channel subfamily N member 4 intermediate conductance calcium-activated potassium channel protein 4|SKCa 4|SKCa4|intermediate-conductance Ca2+-activated K+ channel, KCa3.1|potassium channel, calcium activated intermediate/small conductance subfamily N alpha, member 4|potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4|putative Gardos channel|putative erythrocyte intermediate conductance calcium-activated potassium Gardos channel|small conductance calcium-activated potassium channel 4

For more info on KCNN4, check out the KCNN4 Infographic

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In this infographic, you will see the following information for KCNN4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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