Product Info Summary
SKU: | PB10073 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-KDM5B/PLU1/Jarid1B Antibody Picoband™
View all Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B Antibodies
SKU/Catalog Number
PB10073
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KDM5B/PLU1/Jarid1B Antibody Picoband™ catalog # PB10073. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KDM5B/PLU1/Jarid1B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10073)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human KDM5B, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10073 is reactive to KDM5B in Human, Monkey, Mouse, Rat
Applications
PB10073 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
180 kDa
Calculated molecular weight
175.658kDa
Background of Lysine (K)-specific Demethylase 5B/KDM5B/JARID1B
Lysine-specific demethylase 5B, also known as histone demethylase JARID1B, is a demethylase enzyme that in humans is encoded by the KDM5B gene. This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing resultsi n multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of KDM5B using anti-KDM5B antibody (PB10073).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: monkey COS-7 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM5B antigen affinity purified polyclonal antibody (Catalog # PB10073) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDM5B at approximately 180KD. The expected band size for KDM5B is at 180KD.
Click image to see more details
Figure 2. IHC analysis of KDM5B using anti-KDM5B antibody (PB10073).
KDM5B was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KDM5B Antibody (PB10073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of KDM5B using anti-KDM5B antibody (PB10073).
KDM5B was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KDM5B Antibody (PB10073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of KDM5B using anti-KDM5B antibody (PB10073).
KDM5B was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KDM5B Antibody (PB10073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of KDM5B using anti-KDM5B antibody (PB10073).
KDM5B was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KDM5B Antibody (PB10073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of KDM5B using anti-KDM5B antibody (PB10073).
KDM5B was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KDM5B Antibody (PB10073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of KDM5B using anti-KDM5B antibody (PB10073).
KDM5B was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KDM5B Antibody (PB10073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 8. IF analysis of KDM5B using anti-KDM5B antibody (PB10073).
KDM5B was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-KDM5B Antibody (PB10073) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 9. Flow Cytometry analysis of U20S cells using anti-KDM5B antibody (PB10073).
Overlay histogram showing U20S cells stained with PB10073 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KDM5B Antibody (PB10073,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For KDM5B (Source: Uniprot.org, NCBI)
Gene Name
KDM5B
Full Name
Lysine-specific demethylase 5B
Weight
175.658kDa
Superfamily
JARID1 histone demethylase family
Alternative Names
CT31; CT31FLJ10538; EC 1.14.11; EC 1.14.11.-; homolog 1A; Jarid1B; JARID1BFLJ16281; Jumonji, AT rich interactive domain 1B (RBP2-like); jumonji, AT rich interactive domain 1B; KDM5B; Lysine (K)specific Demethylase 5B; Lysine (K)-specific Demethylase 5B; PLU-1; PLU-1FLJ12459; PLU1FLJ23670; PUT1; RBBP2H1; RBBP2H1AFLJ12491; RBP2-H1; Retinoblastoma-binding protein 2 homolog 1 KDM5B CT31, JARID1B, MRT65, PLU-1, PLU1, PPP1R98, PUT1, RBBP2H1A, RBP2-H1 lysine demethylase 5B lysine-specific demethylase 5B|[histone H3]-trimethyl-L-lysine(4) demethylase 5B|cancer/testis antigen 31|histone demethylase JARID1B|jumonji, AT rich interactive domain 1B|jumonji/ARID domain-containing protein 1B|lysine (K)-specific demethylase 5B|protein phosphatase 1, regulatory subunit 98|putative DNA/chromatin binding motif|retinoblastoma-binding protein 2 homolog 1|retinoblastoma-binding protein 2, homolog 1A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KDM5B, check out the KDM5B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KDM5B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KDM5B/PLU1/Jarid1B Antibody Picoband™ (PB10073)
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No publications found for PB10073
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Customer Q&As
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3 Customer Q&As for Anti-KDM5B/PLU1/Jarid1B Antibody Picoband™
Question
Will anti-KDM5B/PLU1/Jarid1B antibody PB10073 work on dog WB with erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
Our lab technicians have not tested anti-KDM5B/PLU1/Jarid1B antibody PB10073 on dog. You can run a BLAST between dog and the immunogen sequence of anti-KDM5B/PLU1/Jarid1B antibody PB10073 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-10
Question
Is there a BSA free version of anti-KDM5B/PLU1/Jarid1B antibody PB10073 available?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-KDM5B/PLU1/Jarid1B antibody PB10073 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-19
Question
We are currently using anti-KDM5B/PLU1/Jarid1B antibody PB10073 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
The anti-KDM5B/PLU1/Jarid1B antibody (PB10073) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-28