Anti-Krueppel-like factor 8 KLF8 Antibody

Product Name

Anti-Krueppel-like factor 8 KLF8 Antibody

View all KLF8 Antibodies

SKU/Catalog Number

PA1890

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Krueppel-like factor 8 KLF8 Antibody catalog # PA1890. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Krueppel-like factor 8 KLF8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1890)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human KLF8.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1890 is reactive to KLF8 in Human

Applications

PA1890 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

39.314kDa

Background of KLF8

KLF8 (Krueppel-like factor 8) is a protein that in humans is encoded by the KLF8 gene. It is also named as ZNF741, or BKLF3. Kruppel-like factor proteins, including KLF8, all contain a characteristic C-terminal DNA-binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers. The International Radiation Hybrid Mapping Consortium mapped the KLF8 gene to the X chromosome (WI-18614). Suske et al. (2005) stated that the human KLF8 gene maps to chromosome Xp11.21, and the mouse Klf8 gene to chromosome XF3. van Vliet et al. (2000) identified a cDNA encoding KLF8, also called ZNF741. Expression of KLF8 or only its zinc fingers showed that the fingers could bind to a CACCC box. Sequence analysis predicted that the 359-amino acid protein contains a nuclear localization signal upstream of the zinc fingers, a valine-rich hydrophobic stretch, and a pro-val-asp-leu-ser (PVDLS) motif. Yeast 2-hybrid and GST pull-down analysis demonstrated that CTBP2 interacts with KLF8 through the PVDLS motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Anti-KLF8 antibody, PA1890, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: 293T Cell Lysate

Gene/Protein Information For KLF8 (Source: Uniprot.org, NCBI)

Alternative Names

Basic krueppel-like factor 3; basic kruppel-like factor 3; BKLF3DKFZp686O08126; DXS741; Krueppel-like factor 8; Kruppel-like factor 8; Zinc finger protein 741; ZNF741MGC138314 KLF8 BKLF3, ZNF741 Kruppel like factor 8 Krueppel-like factor 8|basic krueppel-like factor 3|basic kruppel-like factor 3|zinc finger protein 741

For more info on KLF8, check out the KLF8 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KLF8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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