Anti-Kelch-like protein 3 KLHL3 Antibody

Product Name

Anti-Kelch-like protein 3 KLHL3 Antibody

View all Kelch-Like 3 Antibodies

SKU/Catalog Number

A05117

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Kelch-like protein 3 KLHL3 Antibody (Catalog # A05117). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Kelch-like protein 3 KLHL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05117)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from N-terminal of human KLHL3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05117 is reactive to KLHL3 in Human, Mouse

Applications

A05117 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

111 kDa

Calculated molecular weight

64.97kDa

Background of Kelch-Like 3

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron. The BCR(KLHL3) complex acts by mediating ubiquitination of WNK4, an inhibitor of potassium channel KCNJ1, leading to WNK4 degradation.

Lai F., Genomics 66:65-75(2000).
Hirosawa M., DNA Res. 6:329-336(1999).
Ota T., Nat. Genet. 36:40-45(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using KLHL3 antibody A05117.

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Western blot analysis of extracts from RAW264.7 cells and 3T3 cells, using KLHL3 antibody A05117.

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Immunofluorescence analysis of A549 cells, using KLHL3 antibody A05117.

Gene/Protein Information For KLHL3 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ40871; kelch-like 3 (Drosophila); kelch-like protein 3; KIAA1129kelch (Drosophila)-like 3; MGC44594 KLHL3 PHA2D kelch like family member 3 kelch-like protein 3

For more info on KLHL3, check out the KLHL3 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KLHL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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