Product Info Summary
SKU: | A06290-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Monkey, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-LAMTOR1 Antibody Picoband™
SKU/Catalog Number
A06290-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LAMTOR1 Antibody Picoband™ catalog # A06290-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat, Monkey.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LAMTOR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06290-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human LAMTOR1 recombinant protein (Position: K31-P161).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06290-1 is reactive to LAMTOR1 in Human, Monkey, Rat
Applications
A06290-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
18 kDa
Calculated molecular weight
17.745kDa
Background of LAMTOR1
Late endosomal/lysosomal adaptor, MAPK and MTOR activator 1 is a protein that in humans is encoded by the LAMTOR1 gene. mTORC1 kinase complex is a critical component in the regulation of cell growth. Its activity is modulated by energy levels, growth factors, and amino acids. The four related GTPases, RagA, RagB, RagC, and RagD, have been shown to interact with raptor in mTORC1. These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals. A protein complex consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3, and LAMTOR3/MAPKSP1 has been identified to interact with and recruit the four Rag GTPases to the surface of lysosomes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of LAMTOR1 using anti-LAMTOR1 antibody (A06290-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: monkey COS-7 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMTOR1 antigen affinity purified polyclonal antibody (Catalog # A06290-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAMTOR1 at approximately 18 kDa. The expected band size for LAMTOR1 is at 18 kDa.
Click image to see more details
Figure 2. IF analysis of LAMTOR1 using anti-LAMTOR1 antibody (A06290-1).
LAMTOR1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-LAMTOR1 Antibody (A06290-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of JK cells using anti-LAMTOR1 antibody (A06290-1).
Overlay histogram showing JK cells stained with A06290-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LAMTOR1 Antibody (A06290-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For LAMTOR1 (Source: Uniprot.org, NCBI)
Gene Name
LAMTOR1
Full Name
Ragulator complex protein LAMTOR1
Weight
17.745kDa
Superfamily
LAMTOR1 family
Alternative Names
c11orf59; chromosome 11 open reading frame 59; FLJ20625; LAMTOR1; Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1; late endosomal/lysosomal adaptor, MAPK and MTOR activator 1; Lipid raft adaptor protein p18; p18; p27kip1 releasing factor from RhoA; p27Kip1-releasing factor from RhoA; p27RF-RhoC11orf59; PDRO; PDROPdro; Protein associated with DRMs and endosomes; ragulator complex protein PDRO; Ragulator1; RhoA activator C11orf59 LAMTOR1 C11orf59, PDRO, Ragulator1, p18, p27RF-Rho late endosomal/lysosomal adaptor, MAPK and MTOR activator 1 ragulator complex protein LAMTOR1|RhoA activator C11orf59|late endosomal/lysosomal adaptor and MAPK and MTOR activator 1|lipid raft adaptor protein p18|p27Kip1-releasing factor from RhoA|p27kip1 releasing factor from RhoA|protein associated with DRMs and endosomes|ragulator complex protein PDRO
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LAMTOR1, check out the LAMTOR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LAMTOR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LAMTOR1 Antibody Picoband™ (A06290-1)
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