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Product Info Summary
| SKU: | A09673 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-LAPTM5 Antibody (N-term)
SKU/Catalog Number
A09673
Size
80 µl
Description
Boster Bio Anti-LAPTM5 Antibody (N-term) (Catalog # A09673). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-LAPTM5 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09673)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This LAPTM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-49 amino acids from the N-terminal region of human LAPTM5.
Reactive Species
A09673 is reactive to LAPTM5 in Human
Calculated molecular weight
29.9 kDa
Background of LAPTM5
RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Its phosphorylation by FYN inhibits its ability to regulate splice site selection. Induces an increased concentration-dependent incorporation of exon in CD44 pre-mRNA by direct binding to purine-rich exonic enhancer. May function as an adapter protein for Src kinases during mitosis. Binds both poly(A) and poly(U) homopolymers. Phosphorylation by PTK6 inhibits its RNA-binding ability (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09673 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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All lanes : Anti-LAPTM5 Antibody (N-term) at 1:1000 dilution
Lane 1: U266B1 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 30 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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LAPTM5 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph node followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LAPTM5 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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LAPTM5 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-LAPTM5 Antibody (N-term) (A09673)
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