Anti-LARP/SEPTIN1 Antibody Picoband™

Product Name

Anti-LARP/SEPTIN1 Antibody Picoband™

View all SEPTIN1 Antibodies

SKU/Catalog Number

A30537-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-LARP/SEPTIN1 Antibody Picoband™ catalog # A30537-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-LARP/SEPTIN1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30537-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human LARP/SEPTIN1, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A30537-1 is reactive to SEPTIN1 in Human, Mouse, Rat

Applications

A30537-1 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

40 kDa

Calculated molecular weight

41.971kDa

Background of SEPTIN1

Septin-1 is a protein that in humans is encoded by the SEPTIN1 gene. This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x10^6 cells, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of LARP/SEPTIN1 using anti-LARP/SEPTIN1 antibody (A30537-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates,
Lane 3: human Daudi whole cell lysates,
Lane 4: rat thymus tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse EL-4 whole cell lysates.
red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARP/SEPTIN1 antigen affinity purified polyclonal antibody (Catalog # A30537-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARP/SEPTIN1 at approximately 40 kDa. The expected band size for LARP/SEPTIN1 is at 40 kDa.

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Figure 2. Flow Cytometry analysis of RAW264.7 cells using anti-LARP/SEPTIN1 antibody (A30537-1).
Overlay histogram showing RAW264.7 cells stained with A30537-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LARP/SEPTIN1 Antibody (A30537-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SEPTIN1 (Source: Uniprot.org, NCBI)

Alternative Names

Septin-1 SEPTIN1 DIFF6, LARP, PNUTL3, SEP1, SEPT1 septin 1 septin-1|differentiation 6 (deoxyguanosine triphosphate triphosphohydrolase)|peanut-like protein 3|serologically defined breast cancer antigen NY-BR-24

For more info on SEPTIN1, check out the SEPTIN1 Infographic

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In this infographic, you will see the following information for SEPTIN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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