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Product Info Summary
| SKU: | M01051 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-LATS1 Antibody (Monoclonal, 31L83)
SKU/Catalog Number
M01051
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-LATS1 Antibody (Monoclonal, 31L83) catalog # M01051. Tested in WB applications. This antibody reacts with Human, Mouse, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LATS1 Antibody (Monoclonal, 31L83) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01051)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
31L83
Immunogen
Recombinant protein within human LATS1 aa 153-598.
Reactive Species
M01051 is reactive to LATS1 in Human, Monkey, Mouse
Observed Molecular Weight
140 kDa
Calculated molecular weight
126.9 kDa
Background of LATS1
Serine/threonine-protein kinase LATS1 is an enzyme that in humans is encoded by the LATS1 gene. It is mapped to 6q25.1. The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01051 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Positive Control
WB: human K562 whole cell lysates
Validation Images & Assay Conditions
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Western blot analysis of LATS1 using anti-LATS1 antibody (M01051).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LATS1 antigen affinity purified monoclonal antibody (M01051) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LATS1 at approximately 140 kDa. The expected band size for LATS1 is at 127 kDa.
Specific Publications For Anti-LATS1 Antibody (Monoclonal, 31L83) (M01051)
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