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Product Info Summary
| SKU: | M00605-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-LEF1 Mouse Monoclonal Antibody [Clone ID: OTI10A7]
SKU/Catalog Number
M00605-2
Size
100 µl
Description
Boster Bio LEF1 mouse monoclonal antibody,clone OTI10A7. Catalog# M00605-2. Tested in IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-LEF1 Mouse Monoclonal Antibody [Clone ID: OTI10A7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00605-2)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI10A7
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human LEF1 (NP_057353) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00605-2 is reactive to LEF1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
44.059kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00605-2 is guaranteed for IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEF1 (1:500).
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Western blot analysis of extracts (35ug) from Colo205 cells by using anti-LEF1 monoclonal antibody (1:500).
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Western blot analysis of extracts (35ug) from 2 different tissues by using anti-LEF1 monoclonal antibody (1:500).
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-LEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-LEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunofluorescent staining of Colo205 cells using anti-LEF1 antibody (M00605-2/green
Protein Target Info & Infographic
Gene/Protein Information For LEF1 (Source: Uniprot.org, NCBI)
Gene Name
LEF1
Full Name
Lymphoid enhancer-binding factor 1
Weight
44.059kDa
Superfamily
TCF/LEF family
Alternative Names
DKFZp586H0919; FLJ46390; LEF1; LEF-1; lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha; TCF10; TCF1ALPHA; TCF1-alpha; TCF7L3 Lef1|3000002B05, AI451430, Lef-, Lef-1|lymphoid enhancer binding factor 1|lymphoid enhancer-binding factor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LEF1, check out the LEF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LEF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LEF1 Mouse Monoclonal Antibody [Clone ID: OTI10A7] (M00605-2)
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