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- Table of Contents
LEF1 antibody (M00605) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunohistochemical analysis of paraffin-embedded human spleen, using LEF1 Antibody.
Designing a Western blot or IHC for LEF1? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-LEF1 Rabbit Monoclonal Antibody
LEF1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
LEF1 antibody was positively detected by WB in:
human Jurkat whole cell, human REH whole cell, mouse thymus tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
LEF1 antibody was positively detected by IHC in:
human spleen tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Recommend
Rat
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-LEF1 Rabbit Monoclonal Antibody
View all LEF1 Antibodies
View all LEF1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CFG-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human LEF1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated Molecular Weight
44.2 kDa
Cite This Product
Anti-LEF1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00605)
Protocols
Western Blot protocol for LEF1 antibody
Immunohistochemistry (IHC) protocol for LEF1 antibody
Review
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Product Promise
We promise Anti-LEF1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00605
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 29, 2026