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Product Info Summary
| SKU: | A04957-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-LEMD3 Antibody Picoband™
SKU/Catalog Number
A04957-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-LEMD3 Antibody Picoband™ catalog # A04957-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LEMD3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04957-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
E.coli-derived human LEMD3 recombinant protein (Position: H535-H852).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04957-1 is reactive to LEMD3 in Human
Applications
A04957-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
120 kDa
Calculated molecular weight
99.997kDa
Background of LEMD3
LEM domain-containing protein 3 (LEMD3), also known as MAN1, is an integral protein in the inner nuclear membrane (INM) of the nuclear envelope. It is encoded by the LEMD3 gene[5] and was first identified after it was isolated from the serum of a patient with a collagen vascular disease. This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MAN1/LEMD3 using anti-MAN1/LEMD3 antibody (A04957-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hacat whole cell lysates,
Lane 2: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAN1/LEMD3 antigen affinity purified polyclonal antibody (Catalog # A04957-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAN1/LEMD3 at approximately 120 kDa. The expected band size for MAN1/LEMD3 is at 100 kDa.
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Figure 2. Flow Cytometry analysis of 293T cells using anti-MAN1/LEMD3 antibody (A04957-1).
Overlay histogram showing 293T cells stained with A04957-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAN1/LEMD3 Antibody (A04957-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For LEMD3 (Source: Uniprot.org, NCBI)
Gene Name
LEMD3
Full Name
Inner nuclear membrane protein Man1
Weight
99.997kDa
Alternative Names
inner nuclear membrane protein Man1; integral inner nuclear membrane protein; LEM domain containing 3; MAN1LEM domain-containing protein 3 LEMD3 MAN1 LEM domain containing 3 inner nuclear membrane protein Man1|LEM domain-containing protein 3|LEMD3/MKX fusion|MAN antigen 1|integral inner nuclear membrane protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LEMD3, check out the LEMD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LEMD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LEMD3 Antibody Picoband™ (A04957-1)
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No publications found for A04957-1
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