Product Info Summary
| SKU: | M00479 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA |
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Product info
Product Name
Anti-Leptin Monoclonal Antibody
SKU/Catalog Number
M00479
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Leptin Monoclonal Antibody catalog # M00479. Tested in ELISA, WB applications. This antibody reacts with Human. Leptin (LEP) is a circulating hormone regulating energy balance and body weight via signaling through the leptin receptor (LEPR), linking nutritional status to endocrine–immune phenotypes. Assay context: monoclonal anti-LEP validated for ELISA and Western blot in human samples; WB workflows emphasize specificity/size controls (cf. Western blotting). Often interpreted alongside vascular mediators such as PLGF/PGF; and, where adipose-tissue inflammation is under study (putative), alongside OPN/SPP1.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Leptin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00479)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
5C11-G6-B12
Isotype
IgG
Immunogen
Purified recombinant full length of human Leptin protein expressed in E.coli.
Reactive Species
M00479 is reactive to LEP in Human
Calculated molecular weight
18.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00479 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA 1:10000-20000
Validation Images & Assay Conditions
Click image to see more details
ELISA validation of LEP using Anti-Leptin Monoclonal Antibody (M00479).
Indirect ELISA assay for anti-Leptin mouse mAb. Antigen coating concentration:4ug/ml.Specific Publications For Anti-Leptin Monoclonal Antibody (M00479)
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