Anti-Lin28 Antibody

Product Name

Anti-Lin28 Antibody

View all LIN28A Antibodies

SKU/Catalog Number

A01966

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Lin28 Antibody (Catalog # A01966). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

Lin28 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Lin28 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01966)

Host

Rabbit

Contents

Lin28 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Lin28 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human Lin28. The immunogen is located within the last 50 amino acids of Lin28.

Reactive Species

A01966 is reactive to LIN28A in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

22.7 kDa

Background of LIN28A

Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01966 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Lin28 antibody can be used for detection of Lin28 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information LIN28A

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