Anti-Lin28B Rabbit Monoclonal Antibody

Product Name

Anti-Lin28B Rabbit Monoclonal Antibody

View all LIN-28B Antibodies

SKU/Catalog Number

M00896

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Lin28B Rabbit Monoclonal Antibody catalog # M00896. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Lin28B Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00896)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

EAA-12

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Lin28B

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00896 is reactive to Lin28b in Human

Applications

M00896 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

35 kDa

Calculated molecular weight

26.903kDa

Background of LIN-28B

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:100-1:500
IP 1:50
FC 1:100

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Lin28B using anti-Lin28B antibody (M00896).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human HUH-7 whole cell lysates,
Lane 5: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Lin28B antigen affinity purified monoclonal antibody (Catalog # M00896) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lin28B at approximately 35 kDa. The expected band size for Lin28B is at 27 kDa.

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Immunofluorescent analysis of MCF-7 cells, using Lin28B Antibody.

Gene/Protein Information For Lin28b (Source: Uniprot.org, NCBI)

Alternative Names

CSDD2; CSDD2protein lin-28 homolog B; FLJ16517; lin-28 homolog B (C. elegans); Lin-28.2; LIN28B; LIN-28B Lin28b|2810403D23Rik, D030047M17Rik, Lin-28.2|lin-28 homolog B (C. elegans)|protein lin-28 homolog B|lin-28B

For more info on Lin28b, check out the Lin28b Infographic

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In this infographic, you will see the following information for Lin28b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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