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Product Info Summary
| SKU: | A09986 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-LYAR Antibody
SKU/Catalog Number
A09986
Size
100μl
Form
Liquid
Description
Boster Bio Anti-LYAR Antibody catalog # A09986. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LYAR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09986)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
2F1
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human LYAR. at AA rangle: 210-290
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09986 is reactive to Lyar in Human
Applications
A09986 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
43.736kDa
Background of Lyar
Cybr (Pscdbp) is a new scaffold protein with significant homology to Tamalin. It contains a PDZ binding domain and a coiled-coil domain. Cybr is highly expressed in the immune system, and its expression seems to be common to nearly all immune cell populations (T cells, B cells, NK Macrophages, dendritic cells). Other organs/tissues in which Cybr is expressed include lung, kidney, intestine and testes. Cybr may play an important in vivo role in cell migration, especially when modulated by pro-inflammatory cytokines. Anti-Cybr Antibody is suitable for researchers interested in the Research Areas of Chromatin, Immunology, Neuroscience, and Signal Transduction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of SH-SY5Y cells using LYAR Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using LYAR Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For Lyar (Source: Uniprot.org, NCBI)
Gene Name
Lyar
Full Name
Cell growth-regulating nucleolar protein
Weight
43.736kDa
Alternative Names
cell growth-regulating nucleolar protein; FLJ20425; Ly1 reactive homolog (mouse); LYAR; PNAS-5; ZLYAR Lyar|MLZ-264|Ly1 antibody reactive clone|cell growth-regulating nucleolar protein|ly1 antibody-reactive protein|protein expressed in male leptotene and zygotene spermatocytes 264
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Lyar, check out the Lyar Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Lyar: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LYAR Antibody (A09986)
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No publications found for A09986
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Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-LYAR Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for promyelocytic leukemia using anti-LYAR antibody A09986. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-08
Question
We are currently using anti-LYAR antibody A09986 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
The anti-LYAR antibody (A09986) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-13
Question
Can you help my question with product A09986, anti-LYAR antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09986 anti-LYAR antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-24
Question
Do you have a BSA free version of anti-LYAR antibody A09986 available?
Verified Customer
Verified customer
Asked: 2017-06-12
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-LYAR antibody A09986 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-12
Question
Will anti-LYAR antibody A09986 work on canine WB with uterus?
M. Walker
Verified customer
Asked: 2015-09-23
Answer
Our lab technicians have not validated anti-LYAR antibody A09986 on canine. You can run a BLAST between canine and the immunogen sequence of anti-LYAR antibody A09986 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-09-23
Question
I was wanting to use your anti-LYAR antibody for WB for human promyelocytic leukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human promyelocytic leukemia identification?
D. Evans
Verified customer
Asked: 2013-06-18
Answer
You can see on the product datasheet, A09986 anti-LYAR antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human promyelocytic leukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-06-18
