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- Table of Contents
LYN antibody (A01424-2) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IHC analysis of Lyn using anti-Lyn antibody (A01424-2).
Lyn was detected in paraffin-embedded section of rat lymphaden tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lyn Antibody (A01424-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Lyn was detected in paraffin-embedded section of rat lymphaden tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lyn Antibody (A01424-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-Lyn Antibody Picoband®
LYN antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
LYN antibody was positively detected by WB in:
human Raji whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
LYN antibody was positively detected by IHC in:
mouse intestine tissue, rat spleen tissue, human tonsil tissue, mouse spleen tissue, rat lymphaden tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Western blot
0.1-0.5μg/ml
Human,
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Mouse
Tested
Recommend
Rat
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Lyn Antibody Picoband®
View all LYN Antibodies
View all LYN Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Lyn, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
59 kDa
Calculated Molecular Weight
58.6 kDa
Background of LYN
Tyrosine-protein kinase Lyn is a protein that in humans is encoded in humans by the LYN gene. It is mapped to 8q12.1. Lyn is a member of the Src family of protein tyrosine kinases. In various hematopoietic cells, Lyn has emerged as a key enzyme involved in the regulation of cell activation.Lyn has been described to have an inhibitory role in myeloid lineage proliferation.
Cite This Product
Anti-Lyn Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01424-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Rat
97%
High Homology
Zebrafish
75%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for LYN antibody
Immunohistochemistry (IHC) protocol for LYN antibody
Publications
Application:
Species:
Denatonium benzoate promotes IgE-mediated mast cell degranulation and ovalbumin-induced allergic diarrhea in BALB/c mice
Published: 2025-03-21
Journal: ASIAN PACIFIC JOURNAL OF ALLERGY AND IMMUNOLOGY
Application: WB
Impact Factor: 1.90
Species: Rat
PMID: 40117235
Review
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1 Review For Anti-Lyn Antibody Picoband®
This antibody is highly specific and efficient, suitable for Western blot detection of LYN protein in HT22 cells, yielding clear and well-defined bands.
| SKU | A01424-2 |
|---|---|
| Application | Western Blot |
| Sample | Mouse HT-22 cells |
| Sample Processing Description | After digestion, cells were collected by centrifugation and lysed in 1 mL of RIPA lysis buffer supplemented with a protease inhibitor cocktail on ice for 1 h. The lysates were then centrifuged, and the supernatants were collected. After protein quantification, 5× loading buffer was added, and the samples were denatured in a 100 °C water bath for 10 min before loading for electrophoresis. |
| Other Reagents | Blocking buffer |
| Primary Antibody | Lyn Antibody Picoband® |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1:2000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | The control represents HT22 cells without drug treatment, while Low, Medium, and High correspond to experimental groups treated with three different drug concentrations for 24 h. The Western blot results obtained with this antibody are clear and well-defined, allowing distinct comparison of expression levels between the groups. It can be observed that the expression of the target protein slightly increases with increasing drug concentration. |
Yu Tian, Institute of Materia Medica
Verified customer
Submitted 2025-12-26
Product Promise
We promise Anti-Lyn Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A01424-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026